FastQCFastQC Report
Thu 26 May 2016
SRR2099022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164685
Sequences flagged as poor quality0
Sequence length43
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAGGCCTCAAGGAAGGAAGGAAGGAGAGAAGGAAGGAG2980.180951513495461No Hit
CATGTCAGGCCTCAAGGAAGGAAGGNAGGAGAGAAGGAAGGAG1720.10444181315845402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC150.00664464653.07031620
TCGCACG306.8863665E-753.07031621
GCACGTA306.8863665E-753.07031623
CGCACGT306.8863665E-753.07031622
CACGGTG203.1048444E-453.07031221
AGCAACG150.00697319252.4270424
CGTATGA307.4918717E-752.4270426
ACGTATG307.4918717E-752.4270425
CACGTAT352.1677297E-644.9374624
CCAAGGC304.2843276E-544.2252621
ACACGGT259.3282474E-442.45625320
CCATCTC351.06312415E-437.90736823
TCAGAGG1550.035.5648355
GTCCGGT357.620092E-735.5648354
TCCGTGT251.5507842E-435.5648355
GCCAGGG357.620092E-735.5648354
TCGGTAG251.5507842E-435.5648355
TGTCCGC1000.035.5648353
TCAGGTA1300.035.564835
GTCAACG200.002230872235.564834
Produced by FastQC (version 0.11.5)