##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164685
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.360306038801347	30.0	27.0	31.0	19.0	35.0
2	25.366226432279806	28.0	21.0	31.0	12.0	35.0
3	28.08139782008076	29.0	27.0	31.0	19.0	35.0
4	27.794808270334276	30.0	26.0	31.0	16.0	37.0
5	26.628600054649787	29.0	24.0	31.0	10.0	37.0
6	28.583107143941465	30.0	27.0	31.0	17.0	37.0
7	23.67010960318183	26.0	17.0	30.0	8.0	34.0
8	26.536029389440447	28.0	24.0	31.0	13.0	35.0
9	25.79855481677141	26.0	26.0	27.0	20.0	31.0
10	25.9571120624222	27.0	26.0	27.0	20.0	31.0
11	25.481003127182195	27.0	26.0	27.0	18.0	31.0
12	27.840878039894342	30.0	26.0	31.0	18.0	35.0
13	27.551003430792118	30.0	26.0	31.0	17.0	35.0
14	31.149691835929197	31.0	31.0	33.0	30.0	34.0
15	31.14475513859793	31.0	31.0	34.0	30.0	34.0
16	31.338318608252116	31.0	31.0	34.0	30.0	34.0
17	35.23315420347937	35.0	35.0	37.0	33.0	37.0
18	35.26801469472022	35.0	35.0	37.0	33.0	37.0
19	35.03354889637794	35.0	35.0	37.0	32.0	37.0
20	34.979299875519935	35.0	35.0	37.0	32.0	37.0
21	34.967027962473814	35.0	35.0	37.0	32.0	37.0
22	36.46346661808908	37.0	35.0	39.0	32.0	39.0
23	36.29666939915597	37.0	35.0	39.0	32.0	39.0
24	36.52715183532198	37.0	35.0	39.0	33.0	39.0
25	36.68159213043082	38.0	35.0	39.0	33.0	39.0
26	22.857230470291768	32.0	2.0	37.0	2.0	39.0
27	29.716240094726295	34.0	17.0	38.0	17.0	40.0
28	34.56256489662082	36.0	32.0	39.0	27.0	40.0
29	36.61703251662264	37.0	35.0	39.0	32.0	40.0
30	37.40230743540699	38.0	36.0	40.0	33.0	41.0
31	37.78786167531955	39.0	37.0	40.0	33.0	41.0
32	38.10914169475058	39.0	37.0	40.0	34.0	41.0
33	38.22053010292376	39.0	38.0	40.0	34.0	41.0
34	38.25495946807542	40.0	38.0	40.0	34.0	41.0
35	38.25256702189028	40.0	38.0	40.0	34.0	41.0
36	38.25256702189028	40.0	38.0	40.0	34.0	41.0
37	38.256483589883715	40.0	38.0	40.0	34.0	41.0
38	38.21384461244193	40.0	38.0	40.0	34.0	41.0
39	38.07485199016304	40.0	37.0	40.0	34.0	41.0
40	38.017463642711846	40.0	37.0	40.0	34.0	41.0
41	37.95153778425479	40.0	37.0	40.0	34.0	41.0
42	37.81833196708868	40.0	37.0	40.0	33.0	41.0
43	37.73176670613596	40.0	37.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.864397919670122
1101	2	4.822866908090116
1101	3	4.755773472529253
1101	4	8.23504105703362
1101	5	8.337928756666102
1101	6	6.179552668071825
1101	7	6.190906359647066
1101	8	5.23036826726419
1101	9	5.884495551639432
1101	10	5.421067222134646
1101	11	3.4855278230426308
1101	12	7.964099552150728
1101	13	6.741819689434873
1101	14	0.32151758816635834
1101	15	1.06116222034683
1101	16	1.617510828757883
1101	17	1.0062649511571138
1101	18	0.4653127938465573
1101	19	0.1866519427978517
1101	20	-0.03403712694309746
1101	21	0.15619963481463373
1101	22	0.3221229053919856
1101	23	1.2370904888307663
1101	24	0.7606941890233827
1101	25	0.32325350220138915
1101	26	12.177047218792964
1101	27	7.333122961548899
1101	28	2.981658104426195
1101	29	1.5985641748920898
1101	30	0.9032287554131742
1101	31	0.5243146097919507
1101	32	0.12752932758073854
1101	33	0.11238232996658581
1101	34	0.19865903760416614
1101	35	0.13637973197974418
1101	36	0.11446207876615944
1101	37	0.12464734111934206
1101	38	-0.09934274700260204
1101	39	0.1722247763506104
1101	40	0.01135018088734796
1101	41	-0.04651839659651813
1101	42	0.029207109450013036
1101	43	-0.0594986851611381
1102	1	3.0526177055807047
1102	2	2.3021023534820166
1102	3	1.1417876313838313
1102	4	4.670758327187624
1102	5	5.112369846768434
1102	6	3.594773142505261
1102	7	4.0172632498776935
1102	8	1.7278701651156467
1102	9	4.339456152831694
1102	10	3.398869716117428
1102	11	3.6057015426549377
1102	12	5.424939079247338
1102	13	3.990088083881968
1102	14	-0.24222373909385198
1102	15	0.9604731090120957
1102	16	1.5918177451759163
1102	17	0.9399019407233453
1102	18	0.33758886208682526
1102	19	0.34461791682230114
1102	20	0.226836532055664
1102	21	0.20124197602772398
1102	22	0.2678237569240949
1102	23	1.238744120641698
1102	24	0.8002342322178961
1102	25	0.629546130299012
1102	26	12.165595377141457
1102	27	7.106340011022507
1102	28	3.0436487004986716
1102	29	1.2864455841865592
1102	30	0.832093163489489
1102	31	0.20653483117853
1102	32	0.041917101297997306
1102	33	0.039928540442723204
1102	34	-0.1691828257622987
1102	35	-0.010469834603945571
1102	36	-0.0251079769629996
1102	37	0.004556008857043992
1102	38	-0.19146357393620406
1102	39	0.021020449837777733
1102	40	-0.22999179134811243
1102	41	-0.30755683029529735
1102	42	-0.14739606010903827
1102	43	-0.3573995731790802
1103	1	-0.6881555911856978
1103	2	-0.8407102527320163
1103	3	-0.1191760009310201
1103	4	1.9461119832944185
1103	5	1.6578687157721035
1103	6	-0.20720287802847537
1103	7	-0.7227505799220104
1103	8	-0.7680148834644562
1103	9	2.606629130809498
1103	10	1.502358810499949
1103	11	-0.8548331314657567
1103	12	2.2192969897755646
1103	13	1.282721957515104
1103	14	-0.2976718597647121
1103	15	1.5571047323727356
1103	16	1.691164931749288
1103	17	0.9809272211283968
1103	18	0.7367567300708373
1103	19	0.3781516650496215
1103	20	0.34204579567742854
1103	21	0.19366137056378108
1103	22	0.14065757664445044
1103	23	1.0803577535425575
1103	24	0.8378301911035422
1103	25	0.25911611728962214
1103	26	11.787823850120603
1103	27	7.2227564520759096
1103	28	2.9805707496530616
1103	29	1.3424093923039493
1103	30	0.7056067530257195
1103	31	0.3137767237514595
1103	32	0.1523304716670637
1103	33	0.16059705889612985
1103	34	0.12979165942927295
1103	35	0.13295456444437548
1103	36	0.021554704846131756
1103	37	0.28157627302966404
1103	38	0.19137809829754104
1103	39	0.046164900923649554
1103	40	-0.1402199830432025
1103	41	0.10112547107713965
1103	42	0.04501630232547171
1103	43	0.10605107904834199
1104	1	1.1679473051648763
1104	2	-1.1157045747851626
1104	3	-0.669114440033546
1104	4	1.636825252955056
1104	5	0.6299642965364143
1104	6	-0.9687888780045668
1104	7	0.09465621209778519
1104	8	0.11190670110109124
1104	9	1.8452224344182788
1104	10	1.2032684773538875
1104	11	0.7649691564479895
1104	12	2.7883348467391187
1104	13	1.0092290041459897
1104	14	0.47647734472504766
1104	15	2.0435123252818777
1104	16	1.9122892847621884
1104	17	0.9229691303529677
1104	18	0.3545077849735989
1104	19	0.24447725645261187
1104	20	0.059284042327441
1104	21	0.013391937043579105
1104	22	0.08633815328477823
1104	23	0.8602566263206839
1104	24	0.4551461359736635
1104	25	0.1879062962346012
1104	26	12.11248765589896
1104	27	7.175898448561565
1104	28	2.7446789533897515
1104	29	1.082753894258353
1104	30	0.7273240032259451
1104	31	0.21345278194144868
1104	32	0.07325520002213182
1104	33	-0.03897022178570353
1104	34	-0.10481632316632528
1104	35	-0.11579449421180499
1104	36	-0.08976413278849549
1104	37	0.07837988778993576
1104	38	-0.09071186428777622
1104	39	-0.16411756199039473
1104	40	-0.24319283648045342
1104	41	-0.1860830111777858
1104	42	-0.17254827684901386
1104	43	-0.33717542919364973
1105	1	0.5371501408632184
1105	2	-2.6271965545698244
1105	3	-1.2057784467874093
1105	4	-1.6682898750098794
1105	5	-2.7765235777306643
1105	6	-2.050381124936994
1105	7	-1.9927785911264912
1105	8	-1.241732175259223
1105	9	1.366921540456282
1105	10	0.584725415678605
1105	11	0.8503523573639242
1105	12	2.8832035280006103
1105	13	0.2554212811469938
1105	14	0.21296556593727445
1105	15	1.6174313020249862
1105	16	1.5618214870543383
1105	17	0.8392998384680155
1105	18	0.4559614851435967
1105	19	0.046512667987379075
1105	20	0.065568375441039
1105	21	-0.06774465526117979
1105	22	-0.2536172064395146
1105	23	1.0566507097494267
1105	24	0.6068675621495245
1105	25	0.17562906393169442
1105	26	11.756519621111927
1105	27	6.786218910229909
1105	28	2.849696936713258
1105	29	1.134538927044673
1105	30	0.5374848701262209
1105	31	0.16589122808978374
1105	32	0.002559343703687489
1105	33	-0.28243088398834004
1105	34	-0.12836233387536566
1105	35	-0.0511678650152092
1105	36	-0.38773681664051196
1105	37	-0.15065710812768174
1105	38	-0.24761896394198857
1105	39	-0.15035077851885603
1105	40	-0.4119423305187766
1105	41	-0.31378195530805186
1105	42	-0.2390992951330233
1105	43	-0.30743529110947776
1106	1	-3.2132301371331096
1106	2	-1.3407554320940847
1106	3	-6.615534544348385
1106	4	0.9516419372114697
1106	5	0.9352519271211612
1106	6	-0.879019991970825
1106	7	-3.2572947201587503
1106	8	-2.522456014755683
1106	9	-0.1444867994335688
1106	10	0.8265297692060116
1106	11	1.0129539833801822
1106	12	2.9333477713785285
1106	13	-4.334193195642936
1106	14	0.14797736766819725
1106	15	1.7621467491794576
1106	16	1.6052518568419067
1106	17	0.6726593978700564
1106	18	0.2754212281281241
1106	19	0.0194998172137133
1106	20	0.05219308039777104
1106	21	0.07639381838161796
1106	22	-0.06662533652081493
1106	23	0.728247877334006
1106	24	0.4911056948534309
1106	25	0.4456526673935315
1106	26	12.11177794788874
1106	27	7.080947470418732
1106	28	3.097061216807667
1106	29	1.2702850586995353
1106	30	0.5982244452639023
1106	31	0.22274934505804822
1106	32	-0.08592741213161759
1106	33	-0.21036164716694117
1106	34	-0.2455404088819222
1106	35	-0.1734337973519544
1106	36	-0.18674022602944973
1106	37	-0.012288369601584748
1106	38	-0.3830503827723035
1106	39	-0.10959546773994333
1106	40	-0.5591230281322055
1106	41	-0.7954499332781566
1106	42	-0.49637702920068705
1106	43	-0.5704827602938494
1107	1	1.2502366331739232
1107	2	-0.472754395690405
1107	3	-4.2219602471596325
1107	4	-23.196745159562724
1107	5	-22.44969430943798
1107	6	-10.528909874079908
1107	7	-6.382738430483922
1107	8	-2.484821606153531
1107	9	0.6518857876891175
1107	10	1.5917584257677433
1107	11	2.4567290837817914
1107	12	3.932013401641516
1107	13	1.89382856883433
1107	14	0.3339470528605766
1107	15	1.6633515684565658
1107	16	1.7499985856927935
1107	17	0.8529936380566099
1107	18	0.40109812928112376
1107	19	-0.010711350035933265
1107	20	0.27891934027599063
1107	21	0.5728959559642064
1107	22	0.684780285969147
1107	23	0.8001613376267898
1107	24	-0.026318842003675513
1107	25	0.28466031087067023
1107	26	12.149917603284521
1107	27	7.04380535356422
1107	28	2.574273290910533
1107	29	1.0353323446174016
1107	30	1.16959379362509
1107	31	0.5355748405885592
1107	32	0.18602491329913562
1107	33	-0.11010254624713411
1107	34	0.3017973291670444
1107	35	0.28865973808009215
1107	36	-0.1665692194223709
1107	37	0.39220703135708845
1107	38	0.27173650672115457
1107	39	0.1093318544520514
1107	40	-0.037850842615945623
1107	41	0.22525223021452945
1107	42	0.01749782505504527
1107	43	0.016043991876124153
1113	1	-9.060004330681497
1113	2	3.4248359657553777
1113	3	-0.03220121101505313
1113	4	1.8032548404372761
1113	5	-22.44969430943798
1113	6	-9.534933970465449
1113	7	-0.47611192445982553
1113	8	0.2651783938464689
1113	9	-20.914379272551848
1113	10	-21.564868080256353
1113	11	-12.32037934995315
1113	12	-21.965576959804267
1113	13	-10.756773840804225
1113	14	0.9544289805714214
1113	15	2.112146749179459
1113	16	2.713854007379542
1113	17	1.075885204321672
1113	18	0.45230294855823416
1113	19	-1.7697474946142435
1113	20	0.1403651234085217
1113	21	-1.2945739235538625
1113	22	0.16670799681251935
1113	23	1.1013661569039002
1113	24	0.9857293507674072
1113	25	0.5918892265333184
1113	26	13.475218808103797
1113	27	9.149227040311207
1113	28	5.306201001753905
1113	29	2.800392585581257
1113	30	1.9858588538660555
1113	31	1.2162977321548212
1113	32	0.896868286793115
1113	33	1.7874878151986522
1113	34	1.2415563653116237
1113	35	1.9211898585620233
1113	36	1.6858404191318428
1113	37	-0.0596001975585736
1113	38	0.1422184344319959
1113	39	1.6033077580665065
1113	40	0.7543178320828474
1113	41	0.8427221097325983
1113	42	2.008461680476735
1113	43	2.8413451966954
1114	1	-2.4894915101686763
1114	2	-2.985420444501031
1114	3	-5.58989351870736
1114	4	-2.2736682364858005
1114	5	1.0118441521004797
1114	6	-1.5862160217475
1114	7	2.709785511437609
1114	8	2.1946655733336478
1114	9	-5.324635682808257
1114	10	0.5633370479487745
1114	11	1.7821847526109522
1114	12	-21.965576959804267
1114	13	-8.641389225419609
1114	14	-0.6096735835311442
1114	15	-27.63785325082054
1114	16	-14.24768445415892
1114	17	-6.577960949524481
1114	18	-2.6694919232366345
1114	19	-1.1543628792296303
1114	20	-1.3468143637709673
1114	21	-0.6407277697077092
1114	22	0.23081056091508145
1114	23	0.6270071825449293
1114	24	1.1908575558956116
1114	25	-0.22862359397949916
1114	26	11.859834192719184
1114	27	6.758201399285564
1114	28	2.363893309446212
1114	29	1.45423873942741
1114	30	0.27432039232759564
1114	31	-0.2708817550246678
1114	32	-0.9236445337197026
1114	33	-1.0073839796731434
1114	34	-0.3097256859704274
1114	35	0.254523191895359
1114	36	-0.2436467603553396
1114	37	-0.0852412231996027
1114	38	0.4050389472525069
1114	39	-0.07617942142067591
1114	40	0.4145742423392562
1114	41	-0.3175343005238105
1114	42	0.258461680476735
1114	43	-0.12019326484305992
1115	1	-1.8484658691430376
1115	2	2.0402205811399945
1115	3	0.17933725052340677
1115	4	-6.965975928793494
1115	5	2.934921075177403
1115	6	1.7727583372268576
1115	7	3.581580383232481
1115	8	1.553639932308009
1115	9	-20.914379272551848
1115	10	-21.564868080256353
1115	11	-8.781917811491612
1115	12	-21.965576959804267
1115	13	-8.102927686958072
1115	14	0.10827513441757475
1115	15	1.5159929030256123
1115	16	-27.786145992620458
1115	17	-15.424114795678328
1115	18	-5.720773974518689
1115	19	-1.3851321099988567
1115	20	-1.6288656458222448
1115	21	-0.25611238509232237
1115	22	1.0513233814279062
1115	23	1.6013661569039002
1115	24	0.908806273844327
1115	25	-0.10041846577437497
1115	26	12.16752650041149
1115	27	6.937688578772747
1115	28	3.056201001753905
1115	29	0.5311618163504903
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2206	5	2.013478495094599
2206	6	-0.47402745488471254
2206	7	0.5911685287979651
2206	8	-0.1930369036039572
2206	9	1.963807696286679
2206	10	1.63909792540937
2206	11	1.1442098851743303
2206	12	2.867284229997434
2206	13	1.6469088787425186
2206	14	0.35102954714366064
2206	15	2.003789808669545
2206	16	1.8838256787676428
2206	17	0.9625707567296047
2206	18	0.5578270278783464
2206	19	0.3761448566605452
2206	20	0.24093169564648065
2206	21	0.34281984415152067
2206	22	0.3380960987955248
2206	23	1.4597230974138142
2206	24	0.7548511637985698
2206	25	0.3751753455134903
2206	26	-8.046027650819717
2206	27	-4.603605820878595
2206	28	-1.858813162552046
2206	29	-0.6231201622941
2206	30	-0.13241310080816504
2206	31	-0.06982124801515255
2206	32	-0.026644461082241833
2206	33	0.04386175287570637
2206	34	-0.06014335140226024
2206	35	0.3291218698934699
2206	36	0.16813503669558116
2206	37	0.25484739450941163
2206	38	0.19250172055097892
2206	39	0.332769514440443
2206	40	0.14171159978823056
2206	41	0.3108524213473274
2206	42	0.5530792442161143
2206	43	0.1317134686500765
2207	1	0.8078300642229621
2207	2	-4.269431550168189
2207	3	-1.221691656874924
2207	4	-0.19674515956272387
2207	5	0.09807639119896194
2207	6	-1.4839785564527084
2207	7	-0.5636915422942188
2207	8	0.6998917696426474
2207	9	1.5919901541997454
2207	10	1.5211191808901425
2207	11	1.5235696946328368
2207	12	1.1172255879664341
2207	13	-0.6293853057723773
2207	14	0.3971041398070909
2207	15	2.037305984848249
2207	16	1.9144909500547023
2207	17	0.9612355227930109
2207	18	0.7118570886856261
2207	19	0.41815059455772996
2207	20	0.2454606648097979
2207	21	0.1926872229429506
2207	22	0.335497805729716
2207	23	1.0695190231459364
2207	24	0.8201242552260055
2207	25	0.5409338125205778
2207	26	6.774581865428637
2207	27	4.727252518018215
2207	28	2.0147997278685565
2207	29	0.9182269804857128
2207	30	0.8791709557768854
2207	31	0.3022849933013205
2207	32	0.20897019762114155
2207	33	0.1378062865362324
2207	34	-0.34443089583487563
2207	35	0.13138094136456857
2207	36	0.1524009286859851
2207	37	-0.08507790456494035
2207	38	0.05782352997339757
2207	39	-0.32662854766597604
2207	40	0.06801209959876786
2207	41	0.16278580400011577
2207	42	-0.07593322398186331
2207	43	0.3636381903259718
2208	1	-24.310004330681497
2208	2	-7.610251753542869
2208	3	-2.282201211015053
2208	4	3.0137811562267487
2208	5	3.9889021817900883
2208	6	2.2019081347977085
2208	7	-20.726111924459826
2208	8	-9.247979500890374
2208	9	3.611936516921837
2208	10	3.066710867112068
2208	11	-21.32037934995315
2208	12	-6.8076822229621605
2208	13	1.462524404809809
2208	14	0.7789903840801919
2208	15	2.1867081526882295
2208	16	1.9682399722918227
2208	17	1.3653588885321994
2208	18	0.7110748783827958
2208	19	0.7214805755611948
2208	20	0.8245756497243093
2208	21	0.6264787080250827
2208	22	0.29828694418093704
2208	23	1.4610152797109208
2208	24	1.3015188244516196
2208	25	0.802415542322791
2208	26	-22.524781191896203
2208	27	-12.82884313512739
2208	28	-4.154325314035567
2208	29	-1.3136425021380447
2208	30	-0.49221132157254033
2208	31	0.16366615320745126
2208	32	0.31792091837206016
2208	33	0.5945053590583029
2208	34	1.1801528565396922
2208	35	1.429961788386585
2208	36	1.3218053314125413
2208	37	1.0807506796344057
2208	38	1.4009903642565575
2208	39	1.2962902142068558
2208	40	1.7060722180477583
2208	41	1.54886246060979
2208	42	1.1882862418802418
2208	43	1.5343276528357492
2209	1	-3.8363201201551824
2209	2	-3.5225324552972523
2209	3	-4.527815246102772
2209	4	-3.126569720966234
2209	5	-2.537413607683593
2209	6	-3.0963374792373806
2209	7	-4.305059292880877
2209	8	-7.598856693872829
2209	9	-1.4231512023764097
2209	10	-0.1964470276247745
2209	11	-1.4958179464443795
2209	12	-1.1761032755937393
2209	13	-2.6427387530849273
2209	14	-0.16837803697243814
2209	15	1.1691642930391097
2209	16	1.2489417266777885
2209	17	0.7688676604620213
2209	18	0.3601976854003368
2209	19	-0.032905389351086
2209	20	0.03510196551378186
2209	21	-0.46124059022052677
2209	22	-0.9648709505558983
2209	23	0.3908398411144276
2209	24	-1.031814508881716
2209	25	-0.03968972083509925
2209	26	0.6857451238932697
2209	27	-0.4428782228466872
2209	28	0.31935889649074767
2209	29	0.0021469715461677197
2209	30	-0.4395797426251704
2209	31	-0.6959829695995623
2209	32	-0.43646504654022067
2209	33	-1.1774244655031012
2209	34	-1.293531353986623
2209	35	-2.5524943519642918
2209	36	-1.0817034405172805
2209	37	-1.7788984431726078
2209	38	-1.4762026181995864
2209	39	-1.510727329652795
2209	40	-0.11848918546101572
2209	41	-2.7142954341270524
2209	42	-1.1450470914530868
2209	43	-0.5709355050589835
2215	1	-2.9975043306814975
2215	2	-1.1376640342446223
2215	3	-0.7822012110150531
2215	4	-2.384245159562724
2215	5	3.36280569056202
2215	6	1.340066029534551
2215	7	3.3988880755401745
2215	8	1.265178393846469
2215	9	-20.914379272551848
2215	10	-10.752368080256353
2215	11	-3.94537934995315
2215	12	-21.965576959804267
2215	13	-8.569273840804225
2215	14	-0.29557101942857855
2215	15	-27.63785325082054
2215	16	-13.786145992620458
2215	17	-4.986614795678328
2215	18	-3.610197051441766
2215	19	-2.7697474946142435
2215	20	-0.9221348765914783
2215	21	0.26792607644613753
2215	22	0.41670799681251935
2215	23	1.2263661569039002
2215	24	1.1107293507674072
2215	25	-0.22061077346668156
2215	26	9.662718808103797
2215	27	6.836727040311207
2215	28	2.431201001753905
2215	29	1.1753925855812568
2215	30	0.6108588538660555
2215	31	0.9037977321548212
2215	32	0.834368286793115
2215	33	0.4749878151986522
2215	34	0.9915563653116237
2215	35	-0.01631014143797671
2215	36	0.3733404191318428
2215	37	-0.1221001975585736
2215	38	-0.2327815655680041
2215	39	-0.7716922419334935
2215	40	-0.18318216791715258
2215	41	-0.2197778902674017
2215	42	-1.054038319523265
2215	43	-1.3461548033046
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	5.0
25	61.0
26	454.0
27	1737.0
28	4326.0
29	8715.0
30	14603.0
31	21138.0
32	27519.0
33	30421.0
34	25340.0
35	18095.0
36	7666.0
37	4456.0
38	149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.9621712530772418	2.31676700709289	0.5910741706680966	96.12998756916177
2	2.462276467194948	92.9793241643137	0.8707532562164132	3.687646112274949
3	0.9175091842001397	0.8531438807420226	97.11327686188784	1.116070073169991
4	94.81889919359818	3.208318411400768	0.876792175556022	1.0959902194450275
5	1.9272137169310506	2.653692777980451	93.99506874819167	1.4240247568968336
6	0.9879586842600633	3.675546352689713	1.4111960554519896	93.92529890759823
7	3.9065975247856577	88.02001133406863	2.6756932976655476	5.397697843480168
8	90.80912044205604	4.787321249658439	1.9928955278258493	2.4106627804596656
9	23.62418967038987	21.012873969017257	34.81449919347475	20.548437167118117
10	22.780422846093508	20.424791890889413	31.970059042789302	24.82472622022778
11	16.52027624208364	19.90198648047349	37.25671861952103	26.321018657921847
12	16.1198801686425	19.638553481759885	45.637198972525425	18.604367377072187
13	18.51983961034169	20.320533320213524	36.40562347789722	24.754003591547562
14	29.420661337739013	28.88493403629826	22.07961806652251	19.61478655944022
15	27.91872296350454	31.607262535794796	23.854871138731493	16.619143361969172
16	21.856047255004434	26.421721744837562	34.30910217922384	17.413128820934162
17	21.601238728481647	30.290554695327444	29.683942071226888	18.42426450496402
18	19.966962626471197	32.297676452369096	27.523047211864306	20.212313709295405
19	21.209581929137443	32.152897956705225	25.519628381455504	21.117891732701825
20	21.54233840361903	31.17952454686219	26.51729058505632	20.76084646446246
21	20.97033731062331	29.363329993624195	27.71958587606643	21.946746819686066
22	20.76388256368218	28.120958192913744	28.406351519567657	22.708807723836415
23	20.943013997098983	29.505909138202224	29.365142510909415	20.185934353789374
24	20.89747092934997	30.728360202811427	28.528402708200506	19.8457661596381
25	21.375352946534292	29.54792482618332	29.25767374077785	19.81904848650454
26	17.808321297115967	33.40725900386712	29.146903974281322	19.63751572473559
27	19.277503506822363	33.467533808196556	28.80877343194943	18.446189253031655
28	20.466344840149375	32.30348847800346	29.267389258280964	17.9627774235662
29	20.06254554287102	32.73257226135536	29.225771192615984	17.979111003157637
30	20.250174575705135	32.4146097094453	29.32143182439202	18.013783890457542
31	21.18893645444333	31.837143637854087	27.647326714637032	19.32659319306555
32	19.86884051370799	33.251358654400825	27.51677444818897	19.36302638370222
33	19.84333728026232	33.329690014269666	28.51018611288217	18.316786592585846
34	19.377599659956886	33.02608009229742	29.41433646051553	18.181983787230166
35	19.121960105656253	33.14630962139843	29.34389895861797	18.38783131432735
36	19.97874730546194	32.85545131614901	29.235813826395844	17.9299875519932
37	19.95263685217233	34.116039712177795	28.83140541032881	17.099918025321067
38	20.23256520023074	33.28839906488144	29.43862525427331	17.040410480614508
39	19.715821113033975	33.65880316968759	29.040289036645717	17.58508668063272
40	20.44509214561132	33.86040015787716	28.827154871421197	16.86735282509032
41	21.641315238181985	32.582809606217936	28.67899322949874	17.096881926101346
42	19.936849136229775	34.15004402343869	28.664419953244074	17.24868688708747
43	20.346115311048365	33.46692169900113	29.329932902207247	16.85703008774327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	14.5
9	26.0
10	56.0
11	86.0
12	86.0
13	209.0
14	332.0
15	535.5
16	739.0
17	1115.0
18	1491.0
19	1491.0
20	1843.5
21	2196.0
22	2750.5
23	3305.0
24	3881.0
25	4457.0
26	4457.0
27	5131.0
28	5805.0
29	6762.0
30	7719.0
31	8597.0
32	9475.0
33	9475.0
34	10592.0
35	11709.0
36	12630.5
37	13552.0
38	14145.0
39	14738.0
40	14738.0
41	15039.0
42	15340.0
43	14894.0
44	14448.0
45	13763.0
46	13078.0
47	13078.0
48	12546.5
49	12015.0
50	10950.5
51	9886.0
52	9234.5
53	8583.0
54	8583.0
55	7301.0
56	6019.0
57	4997.0
58	3975.0
59	3305.5
60	2636.0
61	2636.0
62	2145.0
63	1654.0
64	1225.0
65	796.0
66	577.5
67	359.0
68	359.0
69	250.5
70	142.0
71	109.0
72	76.0
73	49.0
74	22.0
75	22.0
76	16.0
77	10.0
78	9.0
79	8.0
80	5.5
81	3.0
82	3.0
83	2.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.3503658499559766
2	0.0
3	0.0
4	1.6583173938124296
5	3.460545890639706
6	0.0012144396878890002
7	0.35097306979992104
8	0.0
9	0.24288793757780003
10	0.138446124419346
11	0.38011962230925705
12	0.4784892370282661
13	1.2636244952485047
14	0.029753772353280507
15	0.33700701338919753
16	0.07954579955672951
17	0.0
18	0.013966056410723501
19	0.0
20	0.0
21	0.0
22	0.0
23	0.7857424780641831
24	0.0
25	0.0
26	34.83620244709597
27	0.0036433190636670004
28	0.0
29	0.0030360992197225006
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	164685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.85734191468384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.67413020770101	62.41989397785617
2	8.936004351485083	13.021070487745027
3	2.4124654819684577	5.272974674314454
4	1.0316541741456933	3.0065432361377424
5	0.5287052668766925	1.9260030200464673
6	0.3085161672930377	1.3486600732005989
7	0.24081611125124042	1.2281655229197315
8	0.14030683447245917	0.8177906409701525
9	0.0985127705477758	0.6459640746085887
>10	0.5801298665731291	7.481861723923895
>50	0.04278830633616511	2.1503101760589254
>100	0.005970461349232342	0.6807623922182505
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGTCAGGCCTCAAGGAAGGAAGGAAGGAGAGAAGGAAGGAG	298	0.180951513495461	No Hit
CATGTCAGGCCTCAAGGAAGGAAGGNAGGAGAGAAGGAAGGAG	172	0.10444181315845402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0012144396878890002	0.0	0.0
14	0.0	0.0	0.009715517503112002	0.0	0.0
15	0.0	0.0	0.021859914382002004	0.0	0.0
16	0.0	0.0	0.030968212041169504	0.0	0.0
17	0.0	0.0	0.03946928985639251	0.0	0.0
18	0.0	0.0	0.04250538907611501	0.0	0.0
19	0.0	0.0	0.04918480735950451	0.0	0.0
20	0.0	0.0	0.06132920423839451	0.0	0.0
21	0.0	0.0	0.07772414002489601	0.0	0.0
22	0.0	0.0	0.08804687737195252	0.0	0.0
23	0.0	0.0	0.134802805355679	0.0	0.0
24	0.0	0.0	0.17305765552418254	0.0	0.0
25	0.0	0.0	0.19005981115462853	0.0	0.0
26	0.0	0.0	0.21191972553663055	0.0	0.0
27	0.0	0.0	0.26839117102346904	0.0	0.0
28	0.0	0.0	0.30968212041169507	0.0	0.0
29	0.0	0.0	0.34550809120442055	0.0	0.0
30	0.0	0.0	0.40562285575492607	0.0	0.0
31	0.0	0.0	0.4973130521905456	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTGAC	15	0.006644646	53.070316	20
TCGCACG	30	6.8863665E-7	53.070316	21
GCACGTA	30	6.8863665E-7	53.070316	23
CGCACGT	30	6.8863665E-7	53.070316	22
CACGGTG	20	3.1048444E-4	53.070312	21
AGCAACG	15	0.006973192	52.42704	24
CGTATGA	30	7.4918717E-7	52.42704	26
ACGTATG	30	7.4918717E-7	52.42704	25
CACGTAT	35	2.1677297E-6	44.93746	24
CCAAGGC	30	4.2843276E-5	44.22526	21
ACACGGT	25	9.3282474E-4	42.456253	20
CCATCTC	35	1.06312415E-4	37.907368	23
TCAGAGG	155	0.0	35.564835	5
GTCCGGT	35	7.620092E-7	35.564835	4
TCCGTGT	25	1.5507842E-4	35.564835	5
GCCAGGG	35	7.620092E-7	35.564835	4
TCGGTAG	25	1.5507842E-4	35.564835	5
TGTCCGC	100	0.0	35.564835	3
TCAGGTA	130	0.0	35.56483	5
GTCAACG	20	0.0022308722	35.56483	4
>>END_MODULE