FastQCFastQC Report
Thu 26 May 2016
SRR2099021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107866
Sequences flagged as poor quality0
Sequence length47
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACA2150.051.265665
CTGCCAG550.051.265664
TGCCCGA203.676384E-451.2656565
TGCCACA505.456968E-1251.2656565
TGCCAAC150.00761044851.2656565
TGAACGT308.709776E-751.2656565
TGAACGC150.00761044851.2656565
TGACGTG251.784501E-551.2656565
TGACGTC203.676384E-451.2656565
TGACGGG505.456968E-1251.2656565
TGACGCA1200.051.2656565
CTGACAC4050.051.2656564
CTGAACG402.1045707E-951.2656564
CTAACAA203.676384E-451.2656564
TGACCGA1250.051.2656565
TGACAGT750.051.2656565
TGACACG1050.051.2656565
CTGCCGT308.709776E-751.2656564
CTGCCGA451.03682396E-1051.2656564
CTGCCAT402.1045707E-951.2656564
Produced by FastQC (version 0.11.5)