FastQCFastQC Report
Thu 26 May 2016
SRR2099020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80130
Sequences flagged as poor quality0
Sequence length42
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAACAGCTCCAGTTTACAAACAGAGTGTTTCCTAACTGCT1500.18719580681392736No Hit
TGCTACAGCAAGTGCAGATGGTGTTATTATAAATAAGTTCTG1320.16473230999625607No Hit
TGCAACAGCTCCAGTTTACAAACANAGTGTTTCCTAACTGCT1040.12978909272432299No Hit
TGCTACAGCAAGGTGCAGATGGTGTTATTATAAATAAGTTCT930.11606140022463497No Hit
TTCGACAGACTCAAAGAAAGCAAGAAGGAAAATGAGGTAGGT930.11606140022463497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT252.2014281E-549.44526724
GAGTGTT306.461294E-541.2043925
CAAACAG351.4582838E-435.88393819
CAGGTCT406.377013E-834.837916
CAGCGTG301.24062735E-534.837916
CAGGTAC301.24062735E-534.837916
CAGAACG200.002460789234.837916
CAGCGGC552.3646862E-1134.837916
CAGCGCC503.3287506E-1034.837916
CAGCCGG251.7425351E-434.837916
CACGCTC200.002460789234.837916
CAGGGCA251.7425351E-434.837916
CAGATTC251.7425351E-434.837916
CAGGCGC200.002460789234.837916
CAGTGTC358.8761954E-734.837916
AATTCGT301.24062735E-534.837916
CAGCTTA301.24062735E-534.837916
CAGTCGG301.24062735E-534.837916
CAGTCAA358.8761954E-734.837916
CAGTATC200.002460789234.837916
Produced by FastQC (version 0.11.5)