FastQCFastQC Report
Thu 26 May 2016
SRR2099019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100825
Sequences flagged as poor quality0
Sequence length42
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTGAGCACGATCACACGACTACGTTTTTTTTTTTTT6290.6238532110091742No Hit
GACTACGTGAGCACGATCACACGANTACGTTTTTTTTTTTTT3810.3778824696255889No Hit
GACTACGTGAGTACTACGTTTTTTTTTTTTTTTTTTTTTTTT2070.20530622365484752No Hit
GACTACGTGAGTACTACGTTTTTTNTTTTTTTTTTTTTTTTT1390.13786263327547732No Hit
GAATACCTCATGGGTCGGACTGTAGAACTCTGAACCCCTATA1060.1051326555913712Illumina NlaIII expression Adapter 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1250.050.1468219
AAGACTA150.008299733550.14681619
ATCTCTG204.391917E-449.44313423
ATCAACG204.391917E-449.44313424
CACGACT1400.044.77394520
CGACTAC1400.044.77394522
ACGACTA1450.043.23001521
CGATCAA305.961489E-541.78901322
TCAACGG250.001318035439.55450425
GCCTGCG251.648505E-435.179191
GGCTGCG200.00234875435.179191
CTCTACG454.2036845E-935.179191
GACTCCG4300.035.179191
GGCTCCG503.0195224E-1035.179191
GACTACA5100.035.179191
CGTATTG406.0163075E-835.0870066
CGTATGT200.002379214635.0870066
CGTTGTG200.002379214635.0870066
CGTTGGG200.002379214635.0870066
CGTTCGC200.002379214635.0870066
Produced by FastQC (version 0.11.5)