##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100825
Sequences flagged as poor quality	0
Sequence length	42
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.790786015373172	30.0	24.0	31.0	16.0	35.0
2	24.744507810562858	27.0	19.0	31.0	10.0	33.0
3	27.33217951896851	29.0	25.0	31.0	17.0	35.0
4	27.5872353087032	30.0	26.0	31.0	17.0	35.0
5	24.19712372923382	27.0	18.0	30.0	8.0	34.0
6	27.105142573766425	29.0	25.0	31.0	16.0	35.0
7	26.565762459707415	29.0	23.0	31.0	14.0	37.0
8	26.350657079097445	29.0	23.0	31.0	10.0	37.0
9	25.083491197619637	26.0	25.0	27.0	16.0	31.0
10	25.121725762459707	27.0	25.0	27.0	16.0	31.0
11	24.558839573518473	27.0	24.0	27.0	15.0	31.0
12	26.57128688321349	29.0	24.0	31.0	16.0	35.0
13	30.681140590131417	31.0	30.0	31.0	28.0	34.0
14	30.554594594594594	31.0	31.0	31.0	27.0	34.0
15	30.829387552690307	31.0	31.0	33.0	28.0	34.0
16	34.77721795189685	35.0	35.0	37.0	32.0	37.0
17	34.87846268286635	35.0	35.0	37.0	32.0	37.0
18	34.472055541780314	35.0	35.0	37.0	31.0	37.0
19	34.413042400198364	35.0	35.0	37.0	30.0	37.0
20	34.3944359037937	35.0	35.0	37.0	30.0	37.0
21	35.76018844532606	37.0	35.0	39.0	30.0	39.0
22	35.54555913711877	37.0	35.0	39.0	30.0	39.0
23	35.861562112571285	37.0	35.0	39.0	32.0	39.0
24	36.02631291842301	37.0	35.0	39.0	32.0	39.0
25	23.182534093726755	32.0	2.0	37.0	2.0	39.0
26	29.691891891891892	34.0	17.0	38.0	17.0	40.0
27	34.02909000743863	36.0	32.0	39.0	27.0	40.0
28	36.0467741135631	37.0	34.0	39.0	32.0	40.0
29	36.81284403669725	38.0	36.0	39.0	32.0	40.0
30	37.18590627324572	38.0	36.0	40.0	32.0	40.0
31	37.54060004959088	39.0	37.0	40.0	33.0	41.0
32	37.67244235060749	39.0	37.0	40.0	33.0	41.0
33	37.67994049094967	39.0	37.0	40.0	33.0	41.0
34	37.63705430200843	39.0	37.0	40.0	33.0	41.0
35	37.574143317629556	39.0	37.0	40.0	33.0	41.0
36	37.56562360525663	39.0	36.0	40.0	33.0	41.0
37	37.5102405157451	39.0	36.0	40.0	33.0	41.0
38	37.30718571782792	39.0	36.0	40.0	32.0	41.0
39	37.18102653111828	39.0	36.0	40.0	32.0	41.0
40	37.05536325316142	39.0	36.0	40.0	32.0	41.0
41	36.90247458467642	39.0	36.0	40.0	31.0	41.0
42	36.75736176543516	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.354990751254455
1101	2	6.434625382025732
1101	3	4.907883729390914
1101	4	4.908034362384999
1101	5	7.180961683180119
1101	6	7.741059219975316
1101	7	6.532255674830939
1101	8	6.316218773568803
1101	9	5.905504622305234
1101	10	5.747175379142103
1101	11	3.6019880625177514
1101	12	7.948334766627486
1101	13	0.048506474433182234
1101	14	1.1367641205691363
1101	15	1.3464903302046416
1101	16	0.888240854873672
1101	17	0.38381249829944153
1101	18	0.144292721422687
1101	19	0.043178386679052494
1101	20	-0.34522121620825175
1101	21	-0.10984471592389866
1101	22	1.1495406155252894
1101	23	0.29387362807982953
1101	24	0.04452769121948563
1101	25	11.759386037172998
1101	26	6.806453214514285
1101	27	2.9573775824630175
1101	28	1.0681099672702388
1101	29	0.2980781230248297
1101	30	0.3362318694855162
1101	31	-0.061222070108925664
1101	32	-0.2692117225321198
1101	33	-0.0808803938412126
1101	34	-0.21168836749583164
1101	35	-0.3041047573381448
1101	36	-0.40771897285358705
1101	37	-0.34622082491681283
1101	38	-0.40481741652480707
1101	39	-0.6391641546307483
1101	40	-0.6248816263646262
1101	41	-0.21536461122489925
1101	42	-0.5186943933478076
1102	1	4.074433907603478
1102	2	3.1863932929925767
1102	3	2.1467545849438956
1102	4	2.503144108114963
1102	5	4.118891585027246
1102	6	5.3253103212969
1102	7	3.3884559221452584
1102	8	3.7676929987794097
1102	9	4.057863769070828
1102	10	3.437114709847723
1102	11	3.7321565453989614
1102	12	5.130400614065465
1102	13	-0.2330744050783693
1102	14	1.2402883496440253
1102	15	1.6784904847760913
1102	16	0.8818744432498988
1102	17	0.26318880249605314
1102	18	0.0525932083227616
1102	19	0.08570485703997832
1102	20	-0.15014238476841513
1102	21	0.14862773171502397
1102	22	0.8180814610311273
1102	23	0.5385022701696371
1102	24	0.44431708761797495
1102	25	11.781625004635703
1102	26	6.880483278661437
1102	27	2.928009006838934
1102	28	1.1787638045064952
1102	29	0.6242432053341247
1102	30	0.23543196222838247
1102	31	0.26813104837009405
1102	32	0.260659210306585
1102	33	0.4523234859021983
1102	34	-0.010958017391494934
1102	35	0.1801096332639105
1102	36	-0.15825997293087113
1102	37	-0.04206285289424727
1102	38	-0.22214294399988432
1102	39	-0.264560243973051
1102	40	-0.13103550224374771
1102	41	0.1325950456264806
1102	42	0.03516409648911889
1103	1	1.7222479054628472
1103	2	-1.3214421111676913
1103	3	-0.2502508597022022
1103	4	0.7270086434586247
1103	5	0.38839574994883463
1103	6	2.088241355779658
1103	7	-0.9211966908195137
1103	8	0.00565777145380153
1103	9	2.2030717824730317
1103	10	1.3477724882459725
1103	11	-1.5816668060831915
1103	12	2.0893214572875785
1103	13	-0.8137377677290303
1103	14	1.2363095698031756
1103	15	1.5425255724953892
1103	16	1.0003594821069868
1103	17	0.5593286880188799
1103	18	0.5856101006862957
1103	19	0.15565925231197753
1103	20	-0.09138557896079647
1103	21	-0.22454575239312646
1103	22	0.7426358842243914
1103	23	0.2588591969693965
1103	24	0.45115197988843647
1103	25	12.21448600328618
1103	26	7.308650015131256
1103	27	3.0133317300926947
1103	28	1.2879010375094282
1103	29	0.7207460672634838
1103	30	0.34722171512521527
1103	31	0.03526772294778624
1103	32	0.07451746336629128
1103	33	0.24822487731175613
1103	34	0.3293672653112836
1103	35	0.08749248030192547
1103	36	-0.021783170846084943
1103	37	-0.0711475008536695
1103	38	-0.045488609223951926
1103	39	-0.33026828064297575
1103	40	-0.13671864245502263
1103	41	-0.047671602426014204
1103	42	-0.12887913493160852
1104	1	1.3943461883052315
1104	2	0.5766564508873131
1104	3	-1.0842980466350518
1104	4	-0.6122067690780213
1104	5	0.7795056201149677
1104	6	0.7545208476126888
1104	7	-1.0509300427670212
1104	8	-0.44660524683462555
1104	9	2.350758505912932
1104	10	1.0786059379178994
1104	11	0.9561916331182623
1104	12	3.0393479824865146
1104	13	0.15859226158829642
1104	14	1.8434462443808677
1104	15	1.5909466251269677
1104	16	0.7472253204428796
1104	17	-0.15523225013551922
1104	18	-0.26109100220355685
1104	19	-0.008154792082834206
1104	20	-0.11251080582104578
1104	21	-0.06549507530252185
1104	22	0.5084323382241109
1104	23	0.3355198140292899
1104	24	0.05124691217937283
1104	25	11.484607460776058
1104	26	6.3610973137491555
1104	27	2.613973919119637
1104	28	0.9129743308222871
1104	29	0.5700326790183396
1104	30	0.43701090959680755
1104	31	0.2049731536332544
1104	32	0.12030833311360567
1104	33	0.05802524028815981
1104	34	0.0011472457664041258
1104	35	-0.24532896322732256
1104	36	-0.1500143588957883
1104	37	-0.3045189932451251
1104	38	-0.43398504926303616
1104	39	-0.6715777878326961
1104	40	-0.5395442741735721
1104	41	-0.3542470596366769
1104	42	-0.3852745000021045
1105	1	0.8512656929491946
1105	2	1.0068267295250841
1105	3	-1.7766200280592024
1105	4	-1.1765514543205384
1105	5	-1.3631775584196042
1105	6	-2.6011602669383933
1105	7	-2.1413428394646488
1105	8	-5.507461799982202
1105	9	1.829664594664223
1105	10	0.34991140747414207
1105	11	0.8906962770811084
1105	12	2.858857786408084
1105	13	0.2622816320733321
1105	14	1.7770530555263768
1105	15	1.5082118470051924
1105	16	0.6793460634769346
1105	17	0.30562946297077076
1105	18	-0.5646668535967478
1105	19	-0.5622569592654969
1105	20	-0.37950254988709986
1105	21	-0.5261658699361647
1105	22	0.08910829281647636
1105	23	0.5544981422026609
1105	24	-0.03143110639647517
1105	25	11.511243167690399
1105	26	6.136288232221808
1105	27	2.6240874382114825
1105	28	0.6746358375302322
1105	29	-0.15491056143574156
1105	30	-0.15349992631650622
1105	31	-0.10596595885385085
1105	32	-0.15471746667999753
1105	33	-0.28735659665714053
1105	34	-0.4206587397857149
1105	35	-0.21204722948119326
1105	36	-0.33498855910218595
1105	37	-0.31437967435967096
1105	38	-0.1251357200576706
1105	39	-0.20741370114539137
1105	40	-0.35394571896201654
1105	41	0.16845675873277344
1105	42	0.0036883482951068913
1106	1	-3.8064984765483914
1106	2	-4.192686054311853
1106	3	-1.9487101407000011
1106	4	-6.2747247661984815
1106	5	-1.1792273826045978
1106	6	0.7010701997163515
1106	7	-1.4744526791452017
1106	8	-4.796290097258559
1106	9	-0.4538743528296685
1106	10	0.010789588533800298
1106	11	0.71201310168329
1106	12	2.7344239703284607
1106	13	0.08970728318483978
1106	14	1.6734190487321712
1106	15	1.8160818034984274
1106	16	0.9427780320090307
1106	17	0.19316000710331593
1106	18	0.08330829390906302
1106	19	-0.2699049120636374
1106	20	-0.09673093060108329
1106	21	-0.27691084877876193
1106	22	1.0343001993663279
1106	23	0.5205718057106168
1106	24	0.3363276992534452
1106	25	11.471208004689029
1106	26	6.974061639457041
1106	27	3.3570870210233323
1106	28	1.3351787781107234
1106	29	0.6439600506388743
1106	30	0.537743592367562
1106	31	0.05712480936833231
1106	32	0.1553427276104813
1106	33	0.10662546425232478
1106	34	-0.17769134005986587
1106	35	-0.2603665572122793
1106	36	-0.17240437649297036
1106	37	-0.1972913496680988
1106	38	0.024597577516672686
1106	39	0.07143932942853581
1106	40	-0.05836046358326996
1106	41	0.1559113150233742
1106	42	0.291436426249696
1107	1	3.6552717781418913
1107	2	2.26012287556971
1107	3	0.5083698299529686
1107	4	-3.941426369803974
1107	5	-20.471020695674508
1107	6	-23.0496896787031
1107	7	-9.210984489228004
1107	8	-5.575244085310125
1107	9	0.6864935331361615
1107	10	1.6223082972645422
1107	11	2.7890228490892213
1107	12	4.036934189383068
1107	13	0.23599698028158045
1107	14	1.7402883496440253
1107	15	1.5856290207712505
1107	16	0.6091127977568505
1107	17	0.3988512344379771
1107	18	-0.8580238515683476
1107	19	-0.614234646892271
1107	20	0.37413166241851314
1107	21	-0.33329906804299014
1107	22	0.326853390855689
1107	23	0.5778247142107773
1107	24	0.20513075972928618
1107	25	11.416077515222515
1107	26	6.573238874547705
1107	27	2.5285837194826115
1107	28	0.7540814088622128
1107	29	0.6292341309239617
1107	30	0.17281853210134557
1107	31	-0.5376765741344443
1107	32	-0.501742483583314
1107	33	-0.803499260619283
1107	34	-0.8934444057762505
1107	35	-0.9377064586901156
1107	36	-0.31554974909542466
1107	37	-0.4637204451507486
1107	38	-0.5826238877385421
1107	39	-0.34154149019204283
1107	40	-0.7089998095637711
1107	41	-0.28573526290346507
1107	42	-0.5751656070801445
1113	1	-11.361969601168454
1113	2	-8.037290917533738
1113	3	1.2454387954702106
1113	4	2.4680563888167164
1113	5	2.1539793043254924
1113	6	-23.0496896787031
1113	7	-9.254087937503865
1113	8	1.1273421215864268
1113	9	-20.3566099151397
1113	10	-20.94665721997684
1113	11	-11.09459784056595
1113	12	-21.040652017513484
1113	13	-0.15193405420117756
1113	14	2.1152883496440253
1113	15	2.2925255724953892
1113	16	1.3116990046534056
1113	17	0.25660985512763546
1113	18	-0.1381962653614508
1113	19	0.8642136289698001
1113	20	1.0077523520736875
1113	21	1.821873345750113
1113	22	1.826853390855689
1113	23	0.5692040245556029
1113	24	-0.4284899299258882
1113	25	13.204870618670792
1113	26	7.060307840064944
1113	27	3.26134234017227
1113	28	1.6721848571380775
1113	29	1.9137168895446521
1113	30	0.8538530148599648
1113	31	0.15628894310693653
1113	32	0.6663609646925508
1113	33	-0.7603958123434182
1113	34	0.05483145629271746
1113	35	1.5967762999305748
1113	36	0.21893300952526573
1113	37	-0.498203203771439
1113	38	1.869962319158013
1113	39	-1.4794725246748044
1113	40	0.9720346731948482
1113	41	1.1194371508896381
1113	42	0.3860412894715779
1114	1	-8.535046524245377
1114	2	-3.5469063021491216
1114	3	-0.5526381276067127
1114	4	-7.041558995798667
1114	5	-0.7017899264437375
1114	6	0.8349257059122834
1114	7	0.3612966778807518
1114	8	2.290803660047967
1114	9	-2.5873791459089297
1114	10	0.9379581646385446
1114	11	1.9534790825109738
1114	12	-21.040652017513484
1114	13	-0.6134725157396375
1114	14	-27.259711650355975
1114	15	-13.68824365827384
1114	16	-7.370993303038901
1114	17	-3.695313221795441
1114	18	-1.7247347268999107
1114	19	-0.5684786787225065
1114	20	-0.9057091863878526
1114	21	-0.21658819271142704
1114	22	0.5576226216249225
1114	23	1.1845886399402161
1114	24	0.3503562239202651
1114	25	11.656793695593873
1114	26	6.223769378526484
1114	27	2.6074961863261166
1114	28	0.6048771648303841
1114	29	0.02910150492926533
1114	30	-0.030762369755422014
1114	31	0.7043658661838563
1114	32	-0.9201774968459091
1114	33	-0.6257804277280314
1114	34	-0.002860851399589137
1114	35	-0.8839929308386587
1114	36	-1.165682375090121
1114	37	-0.4789724345406725
1114	38	0.05265462685031963
1114	39	-0.2294725246748044
1114	40	-0.06642686526669195
1114	41	-1.1113320798795954
1114	42	0.3764259048561911
1115	1	-7.670793130580218
1115	2	-2.6917026822396224
1115	3	-0.2766200280592024
1115	4	0.6812916829343649
1115	5	2.117214598443141
1115	6	2.832663262473371
1115	7	2.6576767683784865
1115	8	3.2523421215864268
1115	9	-20.3566099151397
1115	10	-20.94665721997684
1115	11	-8.543127252330656
1115	12	-21.040652017513484
1115	13	0.23041888697529345
1115	14	1.3873471731734384
1115	15	-27.45747442750461
1115	16	-15.063300995346594
1115	17	-4.5007430860488356
1115	18	-1.6749609712438058
1115	19	-2.2607863710302
1115	20	-0.07313000086748644
1115	21	0.027755698691287023
1115	22	2.194500449679218
1115	23	1.333909906908545
1115	24	1.4318041877211698
1115	25	13.418105912788437
1115	26	7.920601957712002
1115	27	2.76134234017227
1115	28	1.7971848571380775
1115	29	-0.18922428692593485
1115	30	0.3832647795658488
1115	31	0.3548183548716395
1115	32	-0.046874329425094174
1115	33	-0.3339252241081212
1115	34	0.6724785151162465
1115	35	0.06736453522469077
1115	36	1.3512859507017367
1115	37	-0.027614968477323032
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2206	40	-0.3324207723497068
2206	41	-0.13798859168461775
2206	42	-0.3132161362709951
2207	1	-0.5641227112162994
2207	2	-0.0378890036581403
2207	3	-4.844872209314477
2207	4	-1.7586182523316083
2207	5	-1.4566666286888612
2207	6	0.8689706083782411
2207	7	-1.969398942288553
2207	8	-1.0490932851121393
2207	9	0.6768829078268084
2207	10	1.0437734020327305
2207	11	1.5722681881421856
2207	12	2.021548939424317
2207	13	0.2762956108705943
2207	14	1.8455515075387616
2207	15	1.9779322710599843
2207	16	1.0993784304907237
2207	17	0.19979167330945558
2207	18	0.23441139014094148
2207	19	-0.06939881122158909
2207	20	0.1829915865234497
2207	21	-0.14702617578098653
2207	22	0.5266141564059268
2207	23	0.44958679967521675
2207	24	0.03383064423679372
2207	25	6.504511766996153
2207	26	3.69966190705059
2207	27	1.3259356416076784
2207	28	0.4766106944586497
2207	29	-0.0133166032783123
2207	30	0.16485779954896174
2207	31	-0.25340005210837546
2207	32	0.010260486223650389
2207	33	-0.314223563539592
2207	34	-0.3207666298316809
2207	35	-0.025233269447419104
2207	36	-0.3731722536326316
2207	37	-0.07236588319727844
2207	38	-0.3507314607462959
2207	39	-0.01416151989011638
2207	40	-0.018395948814720953
2207	41	0.38737973462169606
2207	42	0.08400779664861346
2208	1	1.07817124390197
2208	2	-21.16229091753374
2208	3	-9.897166838332605
2208	4	-2.2449717801973677
2208	5	4.07827507897338
2208	6	3.6967891945363363
2208	7	2.921968400524303
2208	8	-23.747657878413573
2208	9	3.784235155282836
2208	10	3.5463005265020335
2208	11	-20.71959784056595
2208	12	-6.364595679485316
2208	13	0.7424321429819223
2208	14	2.2191615890806453
2208	15	1.7819621922136974
2208	16	1.1761356243717174
2208	17	0.9731591509022834
2208	18	0.793141762807565
2208	19	0.6687910937585357
2208	20	0.8158509436229835
2208	21	1.2655353175810973
2208	22	1.485304095081041
2208	23	1.3015983907527868
2208	24	0.8443973940177756
2208	25	-22.170129381329208
2208	26	-12.906241455709704
2208	27	-5.069643575320686
2208	28	-1.2168996499041782
2208	29	-0.5405084625680274
2208	30	-0.2588230414780668
2208	31	0.6017114783182009
2208	32	0.7526285703263511
2208	33	0.9684774270931982
2208	34	1.2872258224899014
2208	35	0.7517058773953664
2208	36	1.0182287841731537
2208	37	1.234191162425745
2208	38	1.519610206481957
2208	39	1.2353162077195634
2208	40	1.6903445323497763
2208	41	0.9504230663825979
2208	42	1.345548331725098
2209	1	-1.6405410297398824
2209	2	-3.519433774676596
2209	3	-2.372418347386933
2209	4	-4.678372182611856
2209	5	-2.0424492671030805
2209	6	-1.0639753929888158
2209	7	-9.382659366075295
2209	8	-7.419086449842144
2209	9	-2.5994670579968435
2209	10	-0.032371505691124014
2209	11	-0.2910264119945225
2209	12	-0.48350916037062674
2209	13	-0.26621976848689144
2209	14	0.4260026353583122
2209	15	1.0853827153525337
2209	16	0.23669900465340277
2209	17	-0.4183901448723617
2209	18	-1.5774819796471675
2209	19	-1.0750720853159166
2209	20	-1.0672476479263153
2209	21	-1.978126654249884
2209	22	-0.38028946628716653
2209	23	-0.5879388325872554
2209	24	-2.2177756442115992
2209	25	-3.5558436670434936
2209	26	-2.228977874220771
2209	27	-1.795800516970587
2209	28	-1.3313865714333488
2209	29	-1.4648545390267742
2209	30	-0.7032898422828922
2209	31	-0.7258539140359233
2209	32	-0.6157818924503076
2209	33	-1.3532529552005599
2209	34	-1.8880256865644256
2209	35	-1.603223700069428
2209	36	-1.0453527047604467
2209	37	-1.4267746323428696
2209	38	-1.9407519665562702
2209	39	-1.3151868103890934
2209	40	-2.027965326805152
2209	41	-1.4234199919675063
2209	42	-1.0389587105284193
2215	1	-12.111969601168454
2215	2	-2.8622909175337377
2215	3	2.07043879547021
2215	4	0.7930563888167157
2215	5	3.828979304325493
2215	6	4.100310321296899
2215	7	2.495912062496135
2215	8	2.9023421215864253
2215	9	-20.3566099151397
2215	10	-9.896657219976838
2215	11	-3.069597840565951
2215	12	-21.040652017513484
2215	13	0.24806594579882102
2215	14	-25.709711650355974
2215	15	-12.40747442750461
2215	16	-4.063300995346594
2215	17	-1.968390144872366
2215	18	-0.11319626536145222
2215	19	0.13921362896979872
2215	20	0.2827523520736861
2215	21	1.021873345750116
2215	22	1.8768533908556861
2215	23	-0.4307959754443971
2215	24	0.1965100700741118
2215	25	7.929870618670794
2215	26	6.485307840064941
2215	27	2.4613423401722727
2215	28	1.897184857138079
2215	29	1.0137168895446536
2215	30	0.25385301485996337
2215	31	0.38128894310693795
2215	32	-2.158639035307452
2215	33	-0.4103958123434168
2215	34	0.15483145629271888
2215	35	0.19677629993057622
2215	36	0.21893300952526573
2215	37	0.501796796228561
2215	38	-0.3050376808419841
2215	39	0.5205274753251956
2215	40	0.47203467319484815
2215	41	0.4194371508896353
2215	42	-0.9889587105284221
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	7.0
25	76.0
26	500.0
27	1920.0
28	4360.0
29	7871.0
30	11386.0
31	14516.0
32	16321.0
33	16305.0
34	12861.0
35	8957.0
36	3764.0
37	1920.0
38	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	94.2766822218877	3.1301118972351847	1.2042751768779165	1.3889307039991972
2	2.5221499705332975	90.7854124838932	2.6749772756784833	4.017460269895019
3	1.8636250929828913	3.050830647160922	0.675427721299281	94.4101165385569
4	1.1465410364492934	1.462930820728986	96.11703446565832	1.273493677163402
5	2.1813251854025233	91.0460445775453	1.2039781867481458	5.56865205030403
6	1.0167956528898403	2.9073357483945332	1.9955129260661946	94.08035567264943
7	90.83154804411649	5.950964056177101	0.7666825359041498	2.450805363802269
8	4.11630740955311	3.5409633018359075	91.22500349601455	1.1177257925964401
9	34.68799553210798	24.16351686928424	21.69321139711382	19.45527620149395
10	30.752234408901806	24.13062085713717	24.338634870712823	20.778509863248203
11	32.6074131845497	21.31665516793153	25.02724700282969	21.048684644689082
12	29.752679224854383	21.81575755145415	24.950626071439313	23.480937152252153
13	29.25641152834962	30.517386775137656	20.063495213056203	20.16270648345652
14	25.589595548940675	26.630440211365386	22.912567050573863	24.867397189120076
15	21.714206233296572	26.35589599912571	31.72384330322991	20.206054464347808
16	21.167369204066453	34.167121249690055	25.6414579717332	19.02405157451029
17	20.129573775696482	32.79625367092626	25.803635209143582	21.27053734423367
18	22.32977932060501	29.336969997520455	23.404909496652614	24.92834118522192
19	22.639226382345647	29.727746094718572	25.53731713364741	22.09571038928837
20	20.21026531118274	28.50285147532854	27.133151500123976	24.15373171336474
21	19.98909000743863	29.20704190428961	27.085544259856185	23.71832382841557
22	20.88544848236013	27.36458406352958	26.779759460839784	24.97020799327051
23	22.739399950409126	29.439127200595088	27.290850483511036	20.530622365484753
24	20.16563352343169	32.12794445821969	27.592363005207044	20.114059013141585
25	17.824398742119794	31.01885343923937	28.042460838882082	23.11428697975876
26	20.09521448053558	30.384329283411855	29.516488966030252	20.003967270022315
27	20.214433071490916	32.564667142743794	28.548758232166943	18.67214155359835
28	19.736568044989735	30.997887386806582	29.0003273062694	20.265217261934282
29	20.597074138358543	30.91693528390776	29.031490205802136	19.454500371931566
30	21.027522935779817	29.919166873295318	30.415075626084796	18.63823456484007
31	21.037441110835605	30.69675179766923	30.30597570047111	17.95983139102405
32	20.789486734440864	31.520952144805353	29.497644433424252	18.19191668732953
33	19.88594098685842	30.951648896603025	30.404165633523434	18.758244483015122
34	20.243987106372426	31.561616662534092	30.566823704438384	17.627572526655094
35	19.32358046119514	32.29159434664022	30.406149268534588	17.978675923630053
36	19.219439623109345	31.715348375898834	31.066699727250185	17.998512273741632
37	19.899826431936525	31.19563600297545	31.05083064716092	17.853706917927102
38	18.555913711877015	32.80238036201339	30.53211009174312	18.109595834366477
39	19.077609719811555	32.062484502851476	31.405901314158196	17.454004463178773
40	19.30771138110588	32.22811802628316	30.392263823456485	18.071906769154474
41	18.498388296553433	32.499876022811804	31.524919414827675	17.47681626580709
42	19.11827423754029	32.78452764691297	30.70369452020828	17.39350359533846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	2.0
6	2.5
7	3.0
8	12.5
9	22.0
10	22.0
11	56.5
12	91.0
13	195.0
14	299.0
15	471.0
16	643.0
17	643.0
18	857.5
19	1072.0
20	1489.5
21	1907.0
22	1907.0
23	2078.0
24	2249.0
25	2568.5
26	2888.0
27	3194.5
28	3501.0
29	3501.0
30	3910.5
31	4320.0
32	4804.0
33	5288.0
34	5288.0
35	5976.5
36	6665.0
37	6860.5
38	7056.0
39	6862.0
40	6668.0
41	6668.0
42	6850.0
43	7032.0
44	7561.5
45	8091.0
46	8578.5
47	9066.0
48	9066.0
49	8857.5
50	8649.0
51	8173.0
52	7697.0
53	7697.0
54	6971.0
55	6245.0
56	5224.5
57	4204.0
58	3467.5
59	2731.0
60	2731.0
61	2328.5
62	1926.0
63	1558.0
64	1190.0
65	893.0
66	596.0
67	596.0
68	450.5
69	305.0
70	228.5
71	152.0
72	152.0
73	138.5
74	125.0
75	89.5
76	54.0
77	51.5
78	49.0
79	49.0
80	39.5
81	30.0
82	19.0
83	8.0
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	1.1703446565831888
2	0.7061740639722291
3	0.0
4	0.0
5	1.9697495660798412
6	3.6270766179023064
7	9.918175055789735E-4
8	0.7051822464666502
9	0.5494668980907512
10	0.3481279444582197
11	0.8073394495412843
12	1.0671956360029755
13	0.029754525167369206
14	0.708157698983387
15	0.17059261095958345
16	0.0
17	0.032729977684106126
18	0.0
19	0.0
20	0.0
21	0.0
22	0.9580957103892883
23	0.0
24	0.0
25	32.82122489461939
26	0.0
27	9.918175055789735E-4
28	0.001983635011157947
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	100825.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.95462434911977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.26796111861691	76.54054054054053
2	5.056253661569364	8.388792462186958
3	1.2147443178421549	3.0230597570047113
4	0.5296572173268451	1.7575006198859409
5	0.23194921029663199	0.9620629804116043
6	0.13869128038355313	0.6903049838829656
7	0.10760530374586018	0.6248450285147533
8	0.06815002570571145	0.4522687825440119
9	0.05738949533112543	0.4284651624101165
>10	0.306077308432669	4.800396727002232
>50	0.015542988318846472	0.8817257624597074
>100	0.004782457944260453	0.826183982147285
>500	0.0011956144860651133	0.6238532110091742
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACTACGTGAGCACGATCACACGACTACGTTTTTTTTTTTTT	629	0.6238532110091742	No Hit
GACTACGTGAGCACGATCACACGANTACGTTTTTTTTTTTTT	381	0.3778824696255889	No Hit
GACTACGTGAGTACTACGTTTTTTTTTTTTTTTTTTTTTTTT	207	0.20530622365484752	No Hit
GACTACGTGAGTACTACGTTTTTTNTTTTTTTTTTTTTTTTT	139	0.13786263327547732	No Hit
GAATACCTCATGGGTCGGACTGTAGAACTCTGAACCCCTATA	106	0.1051326555913712	Illumina NlaIII expression Adapter 1 (95% over 24bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	9.918175055789735E-4	0.0	0.0
12	0.0	0.0	0.01388544507810563	0.0	0.0
13	0.0	0.0	0.03570543020084305	0.0	0.0
14	0.0	0.0	0.05355814530126457	0.0	0.0
15	0.0	0.0	0.08926357550210762	0.0	0.0
16	0.0	0.0	0.11306719563600298	0.0	0.0
17	0.0	0.0	0.11802628316389785	0.0	0.0
18	0.0	0.0	0.14083808579221424	0.0	0.0
19	0.0	0.0	0.1914207785767419	0.0	0.0
20	0.0	0.0	0.2281180262831639	0.0	0.0
21	0.0	0.0	0.28663525911232335	0.0	0.0
22	0.0	0.0	0.39573518472601044	0.0	0.0
23	0.0	0.0	0.485990577733697	0.0	0.0
24	0.0	0.0	0.6615422762211753	0.0	0.0
25	0.0	0.0	0.749814034217704	0.0	0.0
26	0.0	0.0	0.8678403173816018	0.0	0.0
27	0.0	0.0	0.9670220679394992	0.0	0.0
28	0.0	0.0	1.1187701462930821	0.0	0.0
29	0.0	0.0	1.2844036697247707	0.0	0.0
30	0.0	0.0	1.4182990329779321	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	125	0.0	50.14682	19
AAGACTA	15	0.0082997335	50.146816	19
ATCTCTG	20	4.391917E-4	49.443134	23
ATCAACG	20	4.391917E-4	49.443134	24
CACGACT	140	0.0	44.773945	20
CGACTAC	140	0.0	44.773945	22
ACGACTA	145	0.0	43.230015	21
CGATCAA	30	5.961489E-5	41.789013	22
TCAACGG	25	0.0013180354	39.554504	25
GCCTGCG	25	1.648505E-4	35.17919	1
GGCTGCG	20	0.002348754	35.17919	1
CTCTACG	45	4.2036845E-9	35.17919	1
GACTCCG	430	0.0	35.17919	1
GGCTCCG	50	3.0195224E-10	35.17919	1
GACTACA	510	0.0	35.17919	1
CGTATTG	40	6.0163075E-8	35.087006	6
CGTATGT	20	0.0023792146	35.087006	6
CGTTGTG	20	0.0023792146	35.087006	6
CGTTGGG	20	0.0023792146	35.087006	6
CGTTCGC	20	0.0023792146	35.087006	6
>>END_MODULE