FastQCFastQC Report
Thu 26 May 2016
SRR2099018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121250
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCATACAAGGAGTAAAAGAGGTAAAACAGAGAACATAAA1550.12783505154639177No Hit
ACGTGCAGGAGAGGCGAACAAGCGCTCTTCACGCAACTTTTGC1470.12123711340206185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCA150.006955866252.4471220
GATGAAT150.006955866252.4471220
TCCGAAC150.007275031451.8559825
AGTATCA304.9006252E-543.21331826
CAGTATC304.9006252E-543.21331825
CCAGTAT304.9006252E-543.21331824
AAGCGCT259.871256E-441.95769520
GAGAAGT259.871256E-441.95769522
TCCCCTC405.3733183E-639.36026423
GTCCCCT505.8918886E-736.71298222
GCATAAG1500.035.588315
GCAAGGT453.768946E-935.588315
GTACATC405.327638E-835.588313
GGATGTC200.002221251135.588315
GGCGTAG200.002221251135.588314
CGCATAG301.0768921E-535.588314
GCAAAGC301.0768921E-535.588315
GCGCATT453.768946E-935.588313
GCGCATA405.327638E-835.588313
TGCATAT5050.035.588314
Produced by FastQC (version 0.11.5)