FastQCFastQC Report
Thu 26 May 2016
SRR2099016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250088
Sequences flagged as poor quality0
Sequence length43
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCACAAAAGCCGACGATCCAAAAAATGCAGACTTTTTG13960.5582035123636481No Hit
AGTCTGCACAAAAGCCGACGATCCANAAAATGCAGACTTTTTG9100.363871917085186No Hit
AGTCTGACAAGATCAGTCAGACTATCTTGATCGTCGGACTGTA7910.31628866638943093No Hit
AGTCTGACAAGATCAGTCAGACTATNTTGATCGTCGGACTGTA5340.2135248392565817No Hit
AGTCTGCAAAATCAGTTCGTCAGTTCTGTTCATTACAAGTCTC5050.201928921019801No Hit
AGTCTGCAAAAACCGACTCACTATAGGGAGTCGTATTACCGGC3900.15594510732222258No Hit
AGTCTGACAAGATAAGTTCTACAGTCAGACTATCTTGATCGTC3740.14954735932951602No Hit
AGTCTGCAAAATCAGTTCGTCAGTTNTGTTCATTACAAGTCTC3010.12035763411279231No Hit
AGTCTGCAAAAACCGACTCACTATANGGAGTCGTATTACCGGC2970.11875819711461566No Hit
AGTCTGAGTATAAGTCGGACTGTAGAACTCTGAACCCCTATAG2920.11675890086689485Illumina DpnII expression Adapter 1 (95% over 23bp)
AGTCTGACAAGATAAGTTCTACAGTNAGACTATCTTGATCGTC2690.10756213812737915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCA150.006721106452.92963421
GATTCGA251.6291926E-552.1058925
GGATTCG304.7856644E-543.4215724
ATCTTAT250.00102256941.6847124
CCCAATG656.366463E-1140.71510323
GATCATG351.08238084E-437.8068823
TCCCAAT701.4006218E-1037.8068822
GATCCAA4200.037.17676520
TCCAAAA4350.035.8948122
CTGCCTA900.035.4402284
CTGCCAT900.035.4402284
CTGTAAC251.5855832E-435.4402244
TGCCAGC200.002271978235.4402245
TGCCAAT301.1130453E-535.4402245
GGCACAT200.002271978235.4402245
TATGCCA251.5855832E-435.4402243
CTGAAGC251.5855832E-435.4402244
CTGCGGT502.8376235E-1035.4402244
TCAGCAC601.8189894E-1235.4402243
AGCATAA200.002271978235.4402245
Produced by FastQC (version 0.11.5)