##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250088
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.09812146124564	29.0	23.0	31.0	13.0	35.0
2	26.747284955695594	29.0	24.0	31.0	16.0	35.0
3	26.755546047791178	29.0	24.0	31.0	13.0	35.0
4	28.63143773391766	30.0	27.0	31.0	17.0	37.0
5	27.31188621605195	30.0	25.0	31.0	13.0	37.0
6	27.29120149707303	30.0	24.0	31.0	15.0	37.0
7	27.983957646908287	30.0	25.0	31.0	17.0	37.0
8	25.556204216115926	28.0	21.0	31.0	11.0	35.0
9	25.40229039378139	26.0	26.0	27.0	18.0	31.0
10	25.38303317232334	27.0	26.0	27.0	18.0	31.0
11	24.894577108857682	27.0	24.0	27.0	16.0	31.0
12	26.95405217363488	30.0	24.0	31.0	16.0	35.0
13	26.649971210134034	29.0	24.0	31.0	16.0	35.0
14	30.784959694187645	31.0	31.0	33.0	28.0	34.0
15	30.686678289242188	31.0	31.0	33.0	27.0	34.0
16	31.024531364959536	31.0	31.0	34.0	28.0	34.0
17	34.958730526854545	35.0	35.0	37.0	32.0	37.0
18	35.02198826013243	35.0	35.0	37.0	32.0	37.0
19	34.904225712549184	35.0	35.0	37.0	32.0	37.0
20	34.84594222833562	35.0	35.0	37.0	32.0	37.0
21	34.82794856210614	35.0	35.0	37.0	32.0	37.0
22	36.39642845718307	37.0	35.0	39.0	32.0	39.0
23	36.12001375515818	37.0	35.0	39.0	32.0	39.0
24	36.40602907776463	37.0	35.0	39.0	32.0	39.0
25	36.62668340744058	37.0	35.0	39.0	32.0	39.0
26	23.156312977831803	32.0	2.0	37.0	2.0	39.0
27	29.87308067560219	35.0	17.0	38.0	17.0	40.0
28	34.78381609673395	36.0	32.0	39.0	27.0	40.0
29	36.6852667860913	37.0	36.0	40.0	32.0	40.0
30	37.36839032660504	38.0	36.0	40.0	33.0	41.0
31	37.7076309139183	39.0	37.0	40.0	33.0	41.0
32	38.05322926329932	39.0	37.0	40.0	34.0	41.0
33	38.11145676721794	39.0	37.0	40.0	34.0	41.0
34	38.08480214964332	39.0	37.0	40.0	34.0	41.0
35	38.061030517257926	40.0	37.0	40.0	33.0	41.0
36	37.97060634656601	40.0	37.0	40.0	33.0	41.0
37	37.89363344102876	40.0	37.0	41.0	33.0	41.0
38	37.739955535651454	39.0	37.0	41.0	33.0	41.0
39	37.47721601996098	39.0	36.0	40.0	32.0	41.0
40	37.16265474552957	39.0	36.0	40.0	32.0	41.0
41	36.81821598797223	39.0	35.0	40.0	31.0	41.0
42	36.468862960238	38.0	35.0	40.0	31.0	41.0
43	36.178969002910975	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	5.709702926363267
1101	2	4.721669031553883
1101	3	4.417365379359744
1101	4	7.358385603834421
1101	5	8.192613113694158
1101	6	6.3001518460357
1101	7	5.946340190743218
1101	8	5.227523024902638
1101	9	5.5374445123423115
1101	10	5.273892749970344
1101	11	2.865089966974949
1101	12	7.428412936775601
1101	13	6.024915292978385
1101	14	0.23106171502288575
1101	15	0.994610031474604
1101	16	1.6934640318627139
1101	17	0.9062699231056257
1101	18	0.41791724956211596
1101	19	0.27578489451211396
1101	20	0.2887508597944475
1101	21	0.30436371995323697
1101	22	0.21148351810094113
1101	23	1.182661987169105
1101	24	0.8397752991361642
1101	25	0.49797326517793294
1101	26	12.340435442304177
1101	27	7.512173937234028
1101	28	2.993110995759288
1101	29	1.4823327824961439
1101	30	0.756196564380879
1101	31	0.41176044274531165
1101	32	0.15390607928061684
1101	33	0.026079970117265816
1101	34	0.13340811157336674
1101	35	0.02642918575796216
1101	36	-0.03357444509419594
1101	37	-0.2973118803067649
1101	38	-0.12376855132187359
1101	39	-0.07848393477819826
1101	40	-0.3789491056405936
1101	41	-0.384588401480805
1101	42	-0.3884574858747243
1101	43	-0.25406247400407267
1102	1	3.3142455478646227
1102	2	2.1980814138452907
1102	3	1.4521404466390493
1102	4	4.496062982043174
1102	5	5.259358534594469
1102	6	3.4153335364011106
1102	7	3.4108766278630327
1102	8	1.8603857654533371
1102	9	4.436740555787594
1102	10	3.558171375684811
1102	11	3.9478471382001814
1102	12	5.695160290295142
1102	13	4.247714885195727
1102	14	-0.17012587949427527
1102	15	1.38755500497356
1102	16	1.6369735717543925
1102	17	0.8724829074079281
1102	18	0.33040341925574523
1102	19	0.22077530388918376
1102	20	0.20147377534382116
1102	21	0.13305151981118968
1102	22	0.11276049625751483
1102	23	1.114715926571833
1102	24	0.6571717711397582
1102	25	0.40427505333387614
1102	26	12.184953809434191
1102	27	7.057527679026432
1102	28	2.8034220025089596
1102	29	1.3346806732838346
1102	30	0.781255388478705
1102	31	0.37601181852862453
1102	32	0.10594571730103297
1102	33	-0.05353669485835866
1102	34	-0.1149624488378933
1102	35	-0.30010374365794945
1102	36	-0.35085737571799314
1102	37	-0.30819252657463636
1102	38	-0.2901139200414704
1102	39	-0.28809581652122773
1102	40	-0.31643612085513695
1102	41	-0.6582738580379441
1102	42	-0.4952371141354135
1102	43	-0.2469552667838073
1103	1	-0.646365429587398
1103	2	-0.5968264857176742
1103	3	0.08603576981429484
1103	4	2.448701039448199
1103	5	2.0518605705643083
1103	6	-1.4131134693244718
1103	7	-0.11632144247273146
1103	8	-1.813308882588089
1103	9	2.01917712988757
1103	10	1.23485936563862
1103	11	-1.3985505939470038
1103	12	1.6366704514942185
1103	13	1.29963367020353
1103	14	-0.6508634289947608
1103	15	1.3088204767901424
1103	16	1.6800988320787802
1103	17	0.9249643039278155
1103	18	0.3847622597165241
1103	19	0.3113992742479539
1103	20	0.46439052749013854
1103	21	0.22012886179817315
1103	22	0.26015227902108506
1103	23	1.0083108587165341
1103	24	0.9839589174871364
1103	25	0.34935670971189836
1103	26	12.270274933165204
1103	27	7.371007975190107
1103	28	2.8271791284033867
1103	29	1.5029916575742277
1103	30	0.6687237610083443
1103	31	0.3615828536716421
1103	32	0.3163344476640262
1103	33	0.20125793639797251
1103	34	0.21958935804647695
1103	35	0.11125852220551735
1103	36	-0.0863864481239176
1103	37	0.15376436681514605
1103	38	0.05273718373393166
1103	39	-0.176934971795788
1103	40	-0.16458385455650415
1103	41	0.05490306307832782
1103	42	-0.03883484379591806
1103	43	-0.09039384887846325
1104	1	0.7849883389926369
1104	2	-1.3660014240724472
1104	3	-1.1908681019423497
1104	4	1.0861834664343881
1104	5	0.7665780560988296
1104	6	-1.4204278871242728
1104	7	-0.790236544994837
1104	8	-0.0779518191677333
1104	9	2.137253947769956
1104	10	1.1053282950602892
1104	11	0.720882046469491
1104	12	2.812660260476491
1104	13	1.0907742106807916
1104	14	0.36476790646914736
1104	15	1.950420389949393
1104	16	1.979807979411138
1104	17	1.086073056007045
1104	18	0.30317984241478513
1104	19	0.2471307319913052
1104	20	0.265067587377267
1104	21	0.0799044593338536
1104	22	0.16208395365347883
1104	23	0.959386311198358
1104	24	0.6694400163206353
1104	25	0.48928206497678417
1104	26	12.100262875496988
1104	27	7.032073626160521
1104	28	2.6051168946591545
1104	29	1.0630634289020904
1104	30	0.3664498316452054
1104	31	0.04300393447716999
1104	32	0.07442229091646624
1104	33	-0.03883439575513137
1104	34	0.0661100343401273
1104	35	-0.05600570674756966
1104	36	-0.31628989099792193
1104	37	-0.38356385398172677
1104	38	-0.2935711796903604
1104	39	-0.2634639064747262
1104	40	-0.42848183369981996
1104	41	-0.2965540204558721
1104	42	-0.4642896137051764
1104	43	-0.5611228748678982
1105	1	0.896246913984843
1105	2	-2.1732414458996985
1105	3	-1.7110027682971989
1105	4	-0.16418291959616838
1105	5	-2.4928671029282974
1105	6	-2.818904851577031
1105	7	-3.521796414031318
1105	8	-1.440815281905497
1105	9	1.554238278920561
1105	10	0.664409991349654
1105	11	1.0689195425717664
1105	12	2.727338113610262
1105	13	0.24831825495893511
1105	14	0.20978076895745446
1105	15	1.847721216266823
1105	16	1.6248549469820794
1105	17	0.659812142380602
1105	18	0.08902136096794777
1105	19	0.05780854614783948
1105	20	-0.23590099696947675
1105	21	-0.008017469259847587
1105	22	-0.20619442177527247
1105	23	0.8104581852239221
1105	24	0.28679950338019466
1105	25	0.2509849804741293
1105	26	11.954358408705588
1105	27	6.446786558807855
1105	28	2.4930281045126037
1105	29	0.8404809920452152
1105	30	-0.03382885560144189
1105	31	-0.2915883636207397
1105	32	-0.16414763424723589
1105	33	-0.39711023766345477
1105	34	-0.3507320298943597
1105	35	-0.6762592536761645
1105	36	-0.6446984503992326
1105	37	-0.6538437105454946
1105	38	-0.6694968890760791
1105	39	-0.5957916270858874
1105	40	-0.655990993350585
1105	41	-0.9478344227009643
1105	42	-0.7825777334432473
1105	43	-0.7573947021207843
1106	1	-4.689831128343201
1106	2	-2.265271869180122
1106	3	-7.266872477291905
1106	4	-0.6815109619242108
1106	5	0.47854493410874
1106	6	-1.8812229732701518
1106	7	-2.929005408740313
1106	8	-1.58375509672031
1106	9	-0.461667805735523
1106	10	0.16004491198457416
1106	11	0.6682912356934594
1106	12	2.430826870224017
1106	13	-4.195233332342653
1106	14	0.16973447266115826
1106	15	1.8179262427218497
1106	16	1.7771201586222887
1106	17	0.8013796026980629
1106	18	0.3616073398039248
1106	19	0.10923050381979493
1106	20	0.3097008548823723
1106	21	0.07701890640152698
1106	22	-0.20563225246310424
1106	23	0.674413211678953
1106	24	0.4783834980891868
1106	25	0.36027730851645856
1106	26	11.96346885844104
1106	27	6.909336161982456
1106	28	2.5573468875813816
1106	29	0.9089499073891361
1106	30	0.2875005094779226
1106	31	0.2098005252681503
1106	32	-0.17456430091390018
1106	33	-0.3032114281396474
1106	34	0.0047573880950579905
1106	35	-0.19935779864970726
1106	36	-0.5239643234151075
1106	37	-0.6048357740375536
1106	38	-0.4986966245258131
1106	39	-0.4057122620065243
1106	40	-0.38990303038762164
1106	41	-0.4255129941295337
1106	42	-0.6278158286813422
1106	43	-0.5643722153482997
1107	1	1.9180577354698336
1107	2	1.5648016030734446
1107	3	-2.760089458660545
1107	4	-24.222516252929502
1107	5	-23.058492102928298
1107	6	-9.73346350086613
1107	7	-5.863206366637954
1107	8	-2.642557975428403
1107	9	1.2355475206267243
1107	10	1.986645378395469
1107	11	2.620509195020425
1107	12	4.522554345837747
1107	13	2.3182135946113824
1107	14	0.5525236599527155
1107	15	2.0019767454927297
1107	16	1.8944688885302625
1107	17	0.8856908943553279
1107	18	0.10038589493318995
1107	19	0.18552379101355854
1107	20	0.07119813415216214
1107	21	0.04574931589023379
1107	22	-0.3153077709063936
1107	23	1.0243404917010182
1107	24	0.7020789267609189
1107	25	0.3312081242340028
1107	26	11.897619664629758
1107	27	7.149763454542448
1107	28	2.907769415729028
1107	29	1.1867339541305242
1107	30	0.7360072423448472
1107	31	0.5171991561264946
1107	32	0.27172321883332984
1107	33	0.07683822205218149
1107	34	0.0271313192367586
1107	35	0.017021749483390636
1107	36	-0.19952664945136434
1107	37	0.15375897507851732
1107	38	-0.016406446738002955
1107	39	-0.3995436018094267
1107	40	-0.21937468213415912
1107	41	-0.31380006251139037
1107	42	-0.36196161969917284
1107	43	-0.12302463892962834
1113	1	-6.869725308237381
1113	2	0.21356410965585582
1113	3	0.3865666761472468
1113	4	1.221928191514941
1113	5	-23.058492102928298
1113	6	-9.870640962688142
1113	7	-1.100963080697987
1113	8	0.39772638476117095
1113	9	-20.627011721079437
1113	10	-21.09392334198368
1113	11	-11.358163790761566
1113	12	-21.32578688638974
1113	13	-10.357931745041066
1113	14	-0.11730256437587627
1113	15	0.8748045496001566
1113	16	-0.40674227524014483
1113	17	0.257728809047272
1113	18	0.1327713609679506
1113	19	-1.070663676074382
1113	20	-0.4032621080805896
1113	21	0.03469086407348243
1113	22	0.5417222448913961
1113	23	0.7868470741128135
1113	24	1.0114522811579718
1113	25	1.115568313807465
1113	26	13.01373340870559
1113	27	7.250606003252297
1113	28	3.1631669934014894
1113	29	2.1324948809341038
1113	30	0.8113100332874481
1113	31	1.0153560808237074
1113	32	1.3809912546416498
1113	33	1.5539314290032067
1113	34	1.6145457478834189
1113	35	1.5122824129905013
1113	36	1.2101626607118803
1113	37	1.2919896227878382
1113	38	1.042308666479478
1113	39	0.8403194840252226
1113	40	1.1471340066494164
1113	41	0.034804466187921435
1113	42	1.578533377667867
1113	43	1.2266330756569914
1114	1	-3.6337573815478024
1114	2	-0.7818539888550262
1114	3	-7.657534125685512
1114	4	-1.3771554281872334
1114	5	0.8177965568655168
1114	6	-0.06365356291723856
1114	7	1.3028169880305356
1114	8	1.1348397868230258
1114	9	-4.750723061285623
1114	10	0.6998910910060125
1114	11	1.378949611300289
1114	12	-21.32578688638974
1114	13	-8.361368171157906
1114	14	-0.2581960351662538
1114	15	-27.29186211706651
1114	16	-13.796203901815174
1114	17	-6.28350830435479
1114	18	-2.566541353808681
1114	19	-1.3106407665669408
1114	20	-0.8625977323646694
1114	21	-0.6216665242426629
1114	22	-0.2091368616378162
1114	23	0.7501918621998698
1114	24	-0.6340230911215201
1114	25	-0.04422550062552233
1114	26	11.668372583963325
1114	27	6.522656404168682
1114	28	2.219295286643188
1114	29	0.4910286724575883
1114	30	0.27980946054976386
1114	31	-0.4634526247891202
1114	32	-0.7089285620822849
1114	33	-0.9168589490036609
1114	34	-1.182132373536973
1114	35	-1.1835938756692954
1114	36	-0.4061030895744935
1114	37	-0.4004502397551164
1114	38	-0.6696042773922244
1114	39	-0.7879737576700805
1114	40	-0.42331272874577763
1114	41	0.21808052575264725
1114	42	0.6529892768660304
1114	43	-0.3621412656946674
1115	1	-0.6044923333269843
1115	2	0.8300515648529903
1115	3	0.9690039521329119
1115	4	-3.31929044647789
1115	5	2.61892725191041
1115	6	2.4178895032616765
1115	7	2.3882842311299726
1115	8	2.929984449277299
1115	9	-20.627011721079437
1115	10	-21.09392334198368
1115	11	-8.600099274632534
1115	12	-21.32578688638974
1115	13	-7.6912650783744
1115	14	0.248288833473584
1115	15	1.1597507861592966
1115	16	-27.517853386351256
1115	17	-15.242271190952728
1115	18	-5.184432940107321
1115	19	-3.1979038194435603
1115	20	-1.883548846431843
1115	21	-0.21262096388350216
1115	22	0.25677600833225256
1115	23	0.9194635615680085
1115	24	0.26055622381030474
1115	25	0.3897618621945611
1115	26	12.158894699028174
1115	27	7.338419623323983
1115	28	2.6183641260179797
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1115	30	0.7503781336458744
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2206	6	-1.2980266435110366
2206	7	0.25635436507042186
2206	8	-0.3497529919574376
2206	9	1.9522733384989301
2206	10	1.4853617175946887
2206	11	1.2939901964061704
2206	12	3.028933553573598
2206	13	1.637790833632934
2206	14	0.11887219883830014
2206	15	1.9372854539692348
2206	16	1.7855379977000752
2206	17	0.9126325670674404
2206	18	0.3571832155356205
2206	19	0.2448979900830679
2206	20	0.23427735622330914
2206	21	0.2836978912656605
2206	22	0.14468588681012307
2206	23	0.9385936878209336
2206	24	0.7022049036246187
2206	25	0.17010543571397108
2206	26	-7.536679056922274
2206	27	-4.295121667589946
2206	28	-1.6905859345952479
2206	29	-0.6546524864149106
2206	30	-0.2706738754608935
2206	31	-0.06489649072126014
2206	32	0.026932796550198645
2206	33	0.14207686132829167
2206	34	0.23716515922876624
2206	35	0.0947755385633684
2206	36	0.3419479748986589
2206	37	0.35355393687277825
2206	38	0.23301036726367386
2206	39	0.04283501513837251
2206	40	0.053030737477399725
2206	41	0.13583308417346274
2206	42	-0.0431185217108947
2206	43	-0.03632649252713804
2207	1	0.23163177403573343
2207	2	-4.178505440810639
2207	3	-1.516529167788292
2207	4	0.37290359439874265
2207	5	0.3346376680640688
2207	6	-2.4770871034854274
2207	7	-0.6041437422755926
2207	8	0.5313141710207141
2207	9	1.0810035460961345
2207	10	1.1866110091613606
2207	11	1.4681720871010313
2207	12	1.529174945671329
2207	13	-1.517600956236997
2207	14	0.18549641781241633
2207	15	1.9848554401853988
2207	16	1.951612262503705
2207	17	0.795896747978567
2207	18	0.2517281039704926
2207	19	0.16173666319618007
2207	20	0.06153857046054867
2207	21	0.06268068595643683
2207	22	-0.1389393327167454
2207	23	0.9081363022722684
2207	24	0.5269315008356656
2207	25	0.027782054265479417
2207	26	7.635870813285742
2207	27	4.579062322166632
2207	28	1.4294943894998795
2207	29	0.29322431265590154
2207	30	0.09672139885148567
2207	31	0.08108975342760516
2207	32	-0.2598060311938042
2207	33	-0.5306741689611698
2207	34	-0.5328249052124292
2207	35	-0.26252674731484404
2207	36	-0.5269026488725146
2207	37	-0.624677043878826
2207	38	-0.5425089755900743
2207	39	-0.9050155456609517
2207	40	-0.6346726091266675
2207	41	-0.541107323464324
2207	42	-0.6852494891684415
2207	43	-0.7922388497034802
2208	1	-22.314169752681824
2208	2	-8.996753350661603
2208	3	0.4659317555123259
2208	4	2.8370075565943083
2208	5	4.310555516119322
2208	6	3.252374910327731
2208	7	-24.93429641403132
2208	8	-9.887987900953116
2208	9	3.7063216122538947
2208	10	3.322743324682989
2208	11	-20.858163790761566
2208	12	-6.587691648294502
2208	13	2.5825444454351256
2208	14	0.9541260070526931
2208	15	2.708137882933489
2208	16	2.3035751850773174
2208	17	1.7220145233329873
2208	18	0.9899142181108047
2208	19	0.6396537842430732
2208	20	1.1483251935067145
2208	21	1.1180241974068181
2208	22	1.3750555782247247
2208	23	1.9217677090334462
2208	24	1.3051030748087697
2208	25	0.6274730757122242
2208	26	-22.48626659129441
2208	27	-12.991457488811193
2208	28	-3.868579038344542
2208	29	-0.9349654365262126
2208	30	0.2795640015414165
2208	31	0.7137687792364034
2208	32	0.9682928419432386
2208	33	1.065836190907973
2208	34	1.0391489224865893
2208	35	1.000377651085742
2208	36	1.551432501981715
2208	37	1.3038943846926045
2208	38	1.3319912061620158
2208	39	1.6458750395807797
2208	40	2.028086387601796
2208	41	1.9792489106323714
2208	42	2.2531365522710374
2208	43	2.4726648216887384
2209	1	-2.618517578768781
2209	2	-3.5086581125663656
2209	3	-3.4854140001812546
2209	4	-2.164545238436748
2209	5	-1.0367529724935167
2209	6	-6.708805213895872
2209	7	-2.1516877183791436
2209	8	-5.696476513789555
2209	9	-2.6559972283258126
2209	10	-0.20261899415759288
2209	11	-1.7639608922108394
2209	12	-0.5576709443607548
2209	13	-1.379670875475849
2209	14	-0.6680272020570364
2209	15	0.7950944046726178
2209	16	1.105335019445846
2209	17	0.09106214238060772
2209	18	-0.1933155955537913
2209	19	-1.116557395881145
2209	20	-1.5602669389984598
2209	21	-1.342120730129416
2209	22	-1.1104516681520877
2209	23	-0.17450558289201723
2209	24	-0.5561805690835726
2209	25	-1.2974751644534024
2209	26	-3.8848173159320893
2209	27	-2.505432644090696
2209	28	-2.0807943592555134
2209	29	-1.002770819548985
2209	30	-1.4398976961811485
2209	31	-1.7479289433308836
2209	32	-1.754274445841439
2209	33	-1.5040395854895436
2209	34	-2.3806233342421876
2209	35	-3.0239494710674677
2209	36	-1.9661658417035852
2209	37	-2.98337269605274
2209	38	-2.013246889076079
2209	39	-2.220066989404728
2209	40	-1.157213819437544
2209	41	-2.4845192053096596
2209	42	-2.2209835305447
2209	43	-2.0438983253091934
2215	1	-3.2914424799545507
2215	2	0.05952370561545095
2215	3	0.7754555650361361
2215	4	-0.22251625292950195
2215	5	4.077871533435339
2215	6	2.9450156029684216
2215	7	1.7247944950595908
2215	8	2.3749991120338976
2215	9	-20.627011721079437
2215	10	-10.753014251074587
2215	11	-2.880891063488839
2215	12	-21.32578688638974
2215	13	-10.304901442010763
2215	14	0.48875804168472925
2215	15	-27.29186211706651
2215	16	-13.313307931805802
2215	17	-4.855907554589091
2215	18	-2.3369256087290182
2215	19	-0.5908656962764027
2215	20	-0.35275705757553766
2215	21	-0.2910667116840884
2215	22	0.435661638830787
2215	23	0.9509884882542252
2215	24	0.0215532912589822
2215	25	-0.020795322556168117
2215	26	9.01373340870559
2215	27	5.101616104262401
2215	28	1.4182174984519946
2215	29	0.4254241738633979
2215	30	1.2481787201561332
2215	31	0.373941939409562
2215	32	0.060284183934584235
2215	33	0.2963556714274489
2215	34	0.1625255458632111
2215	35	-0.010444859736772116
2215	36	0.5535970041462193
2215	37	0.9662320470302674
2215	38	0.024631898802709884
2215	39	0.4943598880656239
2215	40	0.6774370369524476
2215	41	0.6913701227535825
2215	42	0.7856040847385728
2215	43	0.8276431766670953
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	23.0
25	183.0
26	1165.0
27	3876.0
28	8953.0
29	16281.0
30	25703.0
31	34143.0
32	40736.0
33	42643.0
34	34646.0
35	24864.0
36	10204.0
37	6328.0
38	339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.2046757581958167	93.54645867687358	1.0367392464382148	3.2121263184923947
2	95.63873516522185	2.1936278429992644	0.640174658520201	1.527462333258693
3	1.4762803493170404	2.2504078564345353	95.14690828828253	1.1264035059659
4	0.508097551488434	3.269814386372937	0.4675794054367248	95.75450865670192
5	1.0124369803325048	2.53480252119893	95.59985654394578	0.8529039545227818
6	94.22476358038267	2.9729891836775497	0.6641741807785353	2.1380730551612452
7	1.8989347830447953	11.045758189732993	0.9544825372625328	86.10082448995968
8	2.575893285563482	81.11144877003295	2.230015034707783	14.082642909695785
9	29.026739857606948	34.5831208413478	17.283930787177397	19.106208513867863
10	24.85700428486109	34.03037481511767	22.856066345201878	18.256554554819367
11	28.630723716165495	28.494643079356287	23.828963900496554	19.045669303981665
12	22.915608446411724	37.8552596570157	21.61671867063348	17.612413225939093
13	23.015126323046353	28.192951863417008	27.384727685632022	21.40719412790462
14	27.214293713901732	28.639825288386156	21.70695806601283	22.438922931699278
15	26.58945590541682	27.423492982667792	24.303695660916073	21.68335545099932
16	21.26511329970374	25.95764272559853	33.41780767075026	19.359436303947476
17	22.599245065736863	26.736188861520745	31.64686030517258	19.017705767569815
18	19.778044391121778	28.606278744251153	30.075184963007402	21.540491901619678
19	22.89034259940501	25.04238508045168	28.104107354211315	23.96316496593199
20	22.478487572374526	24.120709510252393	30.817952080867535	22.58285083650555
21	19.144461149675315	28.169284411887013	28.574741690924792	24.111512747512876
22	19.016506189821182	25.771728351620233	31.52050478231662	23.691260676241964
23	20.356785056394013	24.998788094237792	31.478848204763597	23.165578644604594
24	17.01800966059947	27.77662262883465	33.352260004478424	21.853107706087457
25	15.303013339304563	27.44034099996801	38.11418380729983	19.142461853427594
26	14.578083144682488	26.64095726829506	39.27985208960051	19.50110749742194
27	16.9531106294836	26.49693295691744	40.21545277873304	16.33450363486592
28	15.864542597346512	27.42416608686612	41.29019617251665	15.421095143270716
29	16.012347949872442	28.73097623979335	40.274789868922994	14.981885941411216
30	16.2783351393715	28.41531147160788	40.52109865766713	14.78525473135349
31	14.708022775982855	27.071670771888297	42.76414702024887	15.456159431879978
32	16.233485812993827	27.813009820543165	39.29616774895237	16.657336617510637
33	16.182303829052174	27.285195611144875	40.074293848565304	16.458206711237644
34	15.832826844950576	28.32363008221106	41.69532324621733	14.14821982662103
35	16.39342951281149	28.623124660119636	38.95308851284348	16.030357314225395
36	15.913198554108954	29.256501711397586	39.06384952496721	15.766450209526248
37	16.052349572950323	29.969450753334826	38.9071046991459	15.071094974568952
38	14.326956911167269	30.883528997792776	40.48374972009853	14.305764370941429
39	15.221042193148012	27.84979687150123	40.87041361440773	16.058747320943027
40	14.215796039793993	28.617526630626024	42.262723521320495	14.903953808259493
41	15.804436838232942	28.392805732382204	41.06194619493938	14.740811234445475
42	14.771600396660375	29.284891718115226	42.3243018457503	13.619206039474104
43	16.137919452352772	31.37775503022936	37.66874060330764	14.815584914110232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	5.0
7	10.0
8	41.0
9	72.0
10	209.5
11	347.0
12	347.0
13	672.5
14	998.0
15	1609.5
16	2221.0
17	3428.0
18	4635.0
19	4635.0
20	5895.5
21	7156.0
22	9034.5
23	10913.0
24	12120.5
25	13328.0
26	13328.0
27	13831.0
28	14334.0
29	14494.5
30	14655.0
31	14504.5
32	14354.0
33	14354.0
34	14741.5
35	15129.0
36	15759.5
37	16390.0
38	16865.0
39	17340.0
40	17340.0
41	19146.5
42	20953.0
43	20851.0
44	20749.0
45	19044.0
46	17339.0
47	17339.0
48	16291.5
49	15244.0
50	13894.0
51	12544.0
52	11080.0
53	9616.0
54	9616.0
55	8375.5
56	7135.0
57	6020.5
58	4906.0
59	4051.0
60	3196.0
61	3196.0
62	2790.0
63	2384.0
64	2088.5
65	1793.0
66	1572.0
67	1351.0
68	1351.0
69	994.0
70	637.0
71	443.5
72	250.0
73	161.5
74	73.0
75	73.0
76	46.5
77	20.0
78	15.5
79	11.0
80	8.0
81	5.0
82	5.0
83	2.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.3378810658648156
2	0.0
3	0.0
4	1.3135376347525671
5	3.000943667828924
6	0.0011995777486324814
7	0.33708134736572726
8	0.0
9	0.4234509452672659
10	0.24191484597421709
11	0.38826333130737983
12	0.8117142765746457
13	1.3987076549054733
14	0.030389302965356194
15	0.5670004158536196
16	0.12315664885960143
17	0.0
18	0.015194651482678097
19	0.0
20	0.0
21	0.0
22	0.0
23	1.0172419308403442
24	0.0
25	0.0
26	34.469866606954355
27	0.003998592495441604
28	7.99718499088321E-4
29	0.0023991554972649628
30	3.998592495441605E-4
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	250088.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.30171581316473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13550569132815	63.723482855439705
2	6.928955457317904	10.019507367141518
3	1.9441726800167047	4.217010618068599
4	0.9130094090479605	2.640485873109245
5	0.5369700713463131	1.9411928749327962
6	0.3269956272020273	1.4185406946065124
7	0.2351252740606053	1.189997252193565
8	0.16568729690532605	0.9583580683760269
9	0.12606356060764498	0.8203150560104655
>10	0.6346795381568671	7.783536325207336
>50	0.03709719153929914	1.7909718143668405
>100	0.012927809172786063	1.8157617577923855
>500	0.0022483146387454024	1.1135154915736654
>1k	5.620786596863506E-4	0.5673239511813273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTCTGCACAAAAGCCGACGATCCAAAAAATGCAGACTTTTTG	1396	0.5582035123636481	No Hit
AGTCTGCACAAAAGCCGACGATCCANAAAATGCAGACTTTTTG	910	0.363871917085186	No Hit
AGTCTGACAAGATCAGTCAGACTATCTTGATCGTCGGACTGTA	791	0.31628866638943093	No Hit
AGTCTGACAAGATCAGTCAGACTATNTTGATCGTCGGACTGTA	534	0.2135248392565817	No Hit
AGTCTGCAAAATCAGTTCGTCAGTTCTGTTCATTACAAGTCTC	505	0.201928921019801	No Hit
AGTCTGCAAAAACCGACTCACTATAGGGAGTCGTATTACCGGC	390	0.15594510732222258	No Hit
AGTCTGACAAGATAAGTTCTACAGTCAGACTATCTTGATCGTC	374	0.14954735932951602	No Hit
AGTCTGCAAAATCAGTTCGTCAGTTNTGTTCATTACAAGTCTC	301	0.12035763411279231	No Hit
AGTCTGCAAAAACCGACTCACTATANGGAGTCGTATTACCGGC	297	0.11875819711461566	No Hit
AGTCTGAGTATAAGTCGGACTGTAGAACTCTGAACCCCTATAG	292	0.11675890086689485	Illumina DpnII expression Adapter 1 (95% over 23bp)
AGTCTGACAAGATAAGTTCTACAGTNAGACTATCTTGATCGTC	269	0.10756213812737915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.024391414222193788	0.0	0.0
12	3.998592495441605E-4	0.0	0.027990147468091232	0.0	0.0
13	3.998592495441605E-4	0.0	0.03598733245897444	0.0	0.0
14	7.99718499088321E-4	0.0	0.16434215156264995	0.0	0.0
15	7.99718499088321E-4	0.0	0.25311090496145355	0.0	0.0
16	7.99718499088321E-4	0.0	0.32268641438213747	0.0	0.0
17	7.99718499088321E-4	0.0	0.3810658648155849	0.0	0.0
18	7.99718499088321E-4	0.0	0.4498416557371805	0.0	0.0
19	7.99718499088321E-4	0.0	0.5646012603563546	0.0	0.0
20	7.99718499088321E-4	0.0	0.6681648059882921	0.0	0.0
21	7.99718499088321E-4	0.0	0.7989187805892326	0.0	0.0
22	7.99718499088321E-4	0.0	0.9496657176673811	0.0	0.0
23	7.99718499088321E-4	0.0	1.1867822526470682	0.0	0.0
24	7.99718499088321E-4	0.0	1.3027414350148747	0.0	0.0
25	7.99718499088321E-4	0.0	1.4222993506285788	0.0	0.0
26	7.99718499088321E-4	0.0	1.5834426281948755	0.0	0.0
27	7.99718499088321E-4	0.0	1.9892997664821983	0.0	0.0
28	7.99718499088321E-4	0.0	2.3091871661175265	0.0	0.0
29	7.99718499088321E-4	0.0	3.5003678705095806	0.0	0.0
30	7.99718499088321E-4	0.0	3.950609385496305	0.0	0.0
31	7.99718499088321E-4	0.0	4.222513675186335	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACCA	15	0.0067211064	52.929634	21
GATTCGA	25	1.6291926E-5	52.10589	25
GGATTCG	30	4.7856644E-5	43.42157	24
ATCTTAT	25	0.001022569	41.68471	24
CCCAATG	65	6.366463E-11	40.715103	23
GATCATG	35	1.08238084E-4	37.80688	23
TCCCAAT	70	1.4006218E-10	37.80688	22
GATCCAA	420	0.0	37.176765	20
TCCAAAA	435	0.0	35.89481	22
CTGCCTA	90	0.0	35.440228	4
CTGCCAT	90	0.0	35.440228	4
CTGTAAC	25	1.5855832E-4	35.440224	4
TGCCAGC	20	0.0022719782	35.440224	5
TGCCAAT	30	1.1130453E-5	35.440224	5
GGCACAT	20	0.0022719782	35.440224	5
TATGCCA	25	1.5855832E-4	35.440224	3
CTGAAGC	25	1.5855832E-4	35.440224	4
CTGCGGT	50	2.8376235E-10	35.440224	4
TCAGCAC	60	1.8189894E-12	35.440224	3
AGCATAA	20	0.0022719782	35.440224	5
>>END_MODULE