FastQCFastQC Report
Thu 26 May 2016
SRR2099014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117815
Sequences flagged as poor quality0
Sequence length43
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAGGCTTACTTTTTCTAGGATTTTAGAAAATCCTAGAA4640.3938377965454314No Hit
CATGTCAGGCTTACTTTTTCTAGGANTTTAGAAAATCCTAGAA3340.2834953104443407No Hit
CATGTCAGCATTTCGTATCAACCCCTCAAGGGTTGGTACGCGG2790.2368119509400331No Hit
CATGTCAGCATTTCGTATCAACCCCNCAAGGGTTGGTACGCGG1750.14853796205916053No Hit
CATGTCAGGGTTCCCAGCACCGCAGGGGACATGGCCGGGGGCC1480.12562067648431863No Hit
CATGTCAGTCAGAGTGAAAACTACAACCTTGTCGTTCGCTTTG1240.10524975597334804No Hit
CATGTCAGGGTTCCCAGCACCGCAGNGGACATGGCCGGGGGCC1210.10270339090947672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG307.9237907E-751.975521
GCATCGG307.9237907E-751.975520
CGGGCCG308.695679E-751.2811724
ATCGGGC352.2924214E-644.55042622
GGCCGCG352.5151658E-643.95528426
CAATCCG250.001031877241.580420
TCGGGCC405.740816E-638.98162523
CTAGGAT1000.038.98162520
GTATAGG351.2978363E-436.62940626
GACAGCT200.002181880235.7173274
TCCGATT200.002181880235.7173275
TGGCAGG405.15829E-835.7173273
TTAAGGT200.002181880235.7173274
TCATAGC200.002181880235.7173275
TCAAGAG200.002181880235.7173275
TGTCGGT1500.035.7173273
TGTCGGC200.002181880235.7173273
TCGGTGG200.002181880235.7173275
TCCGGCT251.509513E-435.7173235
TGTCACG301.0502332E-535.7173233
Produced by FastQC (version 0.11.5)