FastQCFastQC Report
Thu 26 May 2016
SRR2099013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150242
Sequences flagged as poor quality0
Sequence length47
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTG150.00601157654.4273225
CTGCGTA305.7798206E-754.4273225
CTGCGAG305.7798206E-754.4273225
ACTGCGT202.7397263E-454.427324
CTGAAGT3550.053.660745
CATTGAG150.00646545353.435733
CACTGTC150.00646545353.435733
TCACTGT251.3993278E-553.435732
CAATGAT150.00646545353.435733
GCACTGC7300.052.7037282
CTGATAC1350.052.4114885
CTGACAT1200.052.1595155
CACTGCT2000.052.0998343
ACTGAAT4600.052.0609134
CACTGCG1600.051.7658583
GATCCGG353.979585E-851.7593157
TGACATA353.979585E-851.7593156
GATTAGG459.458745E-1151.7593157
TGATACG401.9444997E-951.759316
TGATACC203.5122925E-451.759316
Produced by FastQC (version 0.11.5)