##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099013_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150242 Sequences flagged as poor quality 0 Sequence length 47 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.297613184063046 32.0 23.0 38.0 17.0 40.0 2 29.123267794624674 31.0 24.0 37.0 12.0 40.0 3 31.54451484937634 32.0 28.0 38.0 22.0 40.0 4 23.420747860119008 25.0 17.0 31.0 10.0 34.0 5 24.23931390689687 30.0 17.0 34.0 2.0 37.0 6 26.851792441527667 30.0 17.0 36.0 10.0 39.0 7 27.056196003780567 31.0 18.0 37.0 7.0 39.0 8 15.177986182292567 16.0 2.0 26.0 2.0 32.0 9 31.35591911715765 32.0 30.0 35.0 25.0 37.0 10 30.990295656341104 32.0 30.0 35.0 25.0 37.0 11 30.81313480917453 32.0 29.0 35.0 24.0 37.0 12 30.35697740977889 32.0 28.0 35.0 23.0 36.0 13 30.614095925240612 32.0 28.0 37.0 21.0 39.0 14 29.992212563730515 32.0 28.0 37.0 17.0 39.0 15 30.537692522729994 32.0 28.0 37.0 17.0 39.0 16 30.443378016799564 32.0 27.0 37.0 18.0 39.0 17 30.27978860771289 31.0 27.0 35.0 18.0 39.0 18 31.16223159968584 31.0 31.0 33.0 30.0 34.0 19 31.242954699751067 31.0 31.0 34.0 30.0 34.0 20 31.439218061527402 31.0 31.0 34.0 30.0 34.0 21 35.32837688529173 35.0 35.0 37.0 33.0 37.0 22 35.41579584936303 35.0 35.0 37.0 33.0 37.0 23 35.043602987180684 35.0 35.0 37.0 32.0 37.0 24 34.95931896540248 35.0 35.0 37.0 32.0 37.0 25 34.924954406890215 35.0 35.0 37.0 32.0 37.0 26 36.472065068356386 37.0 35.0 39.0 32.0 39.0 27 36.077142210566954 37.0 35.0 39.0 32.0 39.0 28 36.51222694053593 38.0 35.0 39.0 33.0 39.0 29 36.77454373610575 38.0 35.0 39.0 33.0 39.0 30 21.602115254056788 32.0 2.0 37.0 2.0 39.0 31 29.007321521279003 34.0 17.0 39.0 17.0 40.0 32 34.40058705288801 36.0 32.0 39.0 27.0 40.0 33 36.68425606687877 37.0 36.0 39.0 32.0 40.0 34 37.614974507794095 38.0 37.0 40.0 34.0 41.0 35 38.104950679570294 39.0 37.0 40.0 34.0 41.0 36 38.49225249930113 40.0 38.0 40.0 35.0 41.0 37 38.6147881417979 40.0 38.0 40.0 35.0 41.0 38 38.61035529345988 40.0 38.0 40.0 35.0 41.0 39 38.5943278177873 40.0 38.0 40.0 35.0 41.0 40 38.50873257810732 40.0 38.0 40.0 35.0 41.0 41 38.469276234341926 40.0 38.0 40.0 35.0 41.0 42 38.341142956030936 40.0 38.0 40.0 34.0 41.0 43 38.089568828955954 40.0 37.0 40.0 34.0 41.0 44 37.87162710826533 39.0 37.0 40.0 33.0 41.0 45 37.64139188775442 39.0 37.0 40.0 33.0 41.0 46 37.37283183131215 39.0 36.0 40.0 33.0 41.0 47 37.22046431756766 39.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.245676184246015 1101 2 5.085359281775531 1101 3 4.967287755668245 1101 4 2.392711459106085 1101 5 10.179358334790926 1101 6 7.192737847095064 1101 7 9.681693195183605 1101 8 8.711410512431737 1101 9 3.120223062689643 1101 10 3.6232758972828307 1101 11 3.4423893566708514 1101 12 3.2913743678390937 1101 13 4.621140714633956 1101 14 5.282253852194536 1101 15 4.555528729450899 1101 16 4.789442006707823 1101 17 4.072906934084852 1101 18 0.17025558533789464 1101 19 -0.5982060640506255 1101 20 -0.09804829095869394 1101 21 0.08315954087095179 1101 22 -0.030151431659326988 1101 23 -0.030199666765732047 1101 24 -0.07886548844943775 1101 25 -0.08810353896411272 1101 26 0.009084961775457145 1101 27 1.3577114592230046 1101 28 0.6990674934838594 1101 29 0.2973462906447679 1101 30 17.254914455968432 1101 31 10.229479872275522 1101 32 4.046213529289076 1101 33 1.7194567888225052 1101 34 0.8728894447368845 1101 35 0.26252516677199367 1101 36 0.051834620286072663 1101 37 -0.30794423857248887 1101 38 -0.16445996229324322 1101 39 -0.018143498436621996 1101 40 -0.07222521728862574 1101 41 -0.12996136905064049 1101 42 -0.01243762646238622 1101 43 -0.16377080177746706 1101 44 -0.19031349578249035 1101 45 -0.24966533433619276 1101 46 -0.2972759036644206 1101 47 -0.20693927460638406 1102 1 5.39859587686551 1102 2 0.20099149480260792 1102 3 2.813369498617071 1102 4 0.8221757950853679 1102 5 9.062952040809051 1102 6 3.480929593984662 1102 7 1.9255744370909404 1102 8 4.232125622491974 1102 9 2.446674112624901 1102 10 2.347607372821873 1102 11 2.3855070732727803 1102 12 1.4017428303735784 1102 13 3.2633675344279105 1102 14 3.8641368393025672 1102 15 3.262625644386439 1102 16 4.259802446948775 1102 17 2.9289109874542163 1102 18 0.22452921591831654 1102 19 0.2261169669689096 1102 20 0.18851295995612816 1102 21 0.24628584273437326 1102 22 0.10304538194198187 1102 23 0.15629684283286593 1102 24 -0.004830274803097723 1102 25 0.13721735277714942 1102 26 0.08225179733251053 1102 27 1.2761359933670064 1102 28 0.5215137300429973 1102 29 0.43763087095214814 1102 30 17.2554337939181 1102 31 10.258299609932447 1102 32 4.210517137913747 1102 33 1.9493510915866779 1102 34 1.05855135432423 1102 35 0.42327644752411686 1102 36 0.18363808816874894 1102 37 -0.08867947473755322 1102 38 0.03167315148258609 1102 39 -0.030904574831374987 1102 40 0.06562945253066488 1102 41 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30 17.209537027317317 1103 31 10.485177712441615 1103 32 4.222986018520899 1103 33 1.8176583474974777 1103 34 0.8925375033653751 1103 35 0.29777740562421684 1103 36 0.26617954297207547 1103 37 0.007912103034527718 1103 38 0.06556273610470242 1103 39 0.00617012679965967 1103 40 0.22164737730094686 1103 41 0.10430856370632569 1103 42 0.046171496279399094 1103 43 -0.026621736052824474 1103 44 -0.29767381929276127 1103 45 -0.02854381656482019 1103 46 -0.007648606710503714 1103 47 -0.1086079554653594 1106 1 -6.582749540882741 1106 2 -3.0362296763883805 1106 3 -3.9377678149470974 1106 4 -0.5922551081659826 1106 5 -19.0773620953166 1106 6 -10.68186683348685 1106 7 -3.3417492463091207 1106 8 -11.332272283267187 1106 9 -8.363128342388517 1106 10 -3.1028919892266096 1106 11 -2.2377407598918992 1106 12 -3.614483136452943 1106 13 -1.142853592766425 1106 14 -0.9768328470012726 1106 15 -3.6803018931207134 1106 16 -2.0045118651635043 1106 17 -6.053956714600428 1106 18 1.1626259205687646 1106 19 1.189925317534268 1106 20 0.5319948553320835 1106 21 0.5263069611293716 1106 22 0.01334499917107479 1106 23 0.46997540326227494 1106 24 -0.019753940604495313 1106 25 0.6321797356360506 1106 26 0.27813371030712375 1106 27 2.0876631173055884 1106 28 1.4005053266816532 1106 29 0.7087382667900641 1106 30 17.63601366984753 1106 31 10.621738388585275 1106 32 4.7016870090035425 1106 33 2.6408729359265095 1106 34 1.6518550632423512 1106 35 1.092887957049399 1106 36 0.185802723656451 1106 37 0.3983899079893618 1106 38 0.5052619587332359 1106 39 0.38034976983843194 1106 40 0.6092433378753768 1106 41 0.7044223844507655 1106 42 0.6340677498817016 1106 43 0.42041529201488714 1106 44 0.24791502143084898 1106 45 0.24595791496352604 1106 46 0.5326972146151476 1106 47 0.6125917636153559 1107 1 -4.592750235375416 1107 2 -0.2577381144743569 1107 3 -1.0378442091411415 1107 4 0.33767058111799386 1107 5 -19.0773620953166 1107 6 -8.399659735771731 1107 7 -2.0729111325512193 1107 8 -11.332272283267187 1107 9 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0.9192670542289463 2209 20 0.8152581606542064 2209 21 0.7195842720537442 2209 22 0.10963351457723292 2209 23 -0.00936633343240345 2209 24 0.27261300617421824 2209 25 0.4441545255520154 2209 26 -0.49427525327831034 2209 27 1.626528663524077 2209 28 0.33818039670966016 2209 29 1.0312172583867039 2209 30 -4.890246834354151 2209 31 -2.5312728158965214 2209 32 -0.7790552879152202 2209 33 -1.2852475122527593 2209 34 0.11684105764010866 2209 35 -0.5667759084968225 2209 36 0.2883937600710169 2209 37 0.7646364065887994 2209 38 0.03782498394331668 2209 39 -1.2856166167161902 2209 40 -0.6078434968585142 2209 41 -0.5466280357173048 2209 42 -0.6159322501182984 2209 43 0.2159334992978046 2209 44 0.07984779454009328 2209 45 0.43783466566380724 2209 46 0.9493638812818119 2209 47 1.0632220157161996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 17.0 26 218.0 27 1255.0 28 4244.0 29 10268.0 30 19222.0 31 23430.0 32 18795.0 33 16595.0 34 16976.0 35 17391.0 36 13335.0 37 7687.0 38 809.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.23561986661519416 0.6143421945927238 98.90509977236725 0.2449381664248346 2 96.0776613729849 2.2350607686266155 1.3325168727785839 0.35476098560988273 3 0.4552655049852904 0.6602681007973802 0.20433700296854407 98.68012939124878 4 0.3773911422904381 90.16985929367287 1.175436961701788 8.277312602334899 5 0.09537591264881931 3.592218641057686 0.4826350062487667 95.82977044004473 6 0.1933830539646907 1.2333240924181672 98.11552947063079 0.4577633829863482 7 97.0975768371646 1.2342013741623548 0.7761472559165323 0.8920745327565245 8 2.1055402026582444 81.38476867256969 3.147030624330269 13.362660500441786 9 25.108167476535982 32.93350196365573 20.98315915596086 20.975171403847433 10 29.894017692914076 28.786357725658767 22.35397955814878 18.965645023278377 11 21.016759627800482 34.76524540408141 24.784015122269405 19.433979845848697 12 26.02201781126449 31.023948030510777 23.19524500472571 19.75878915349902 13 25.629456252621324 31.296930174664812 20.741953377619634 22.331660195094234 14 25.80512806247696 32.20373164947809 22.80367462464446 19.187465663400488 15 30.900099175314 30.05544499098104 20.129926317400944 18.91452951630402 16 24.7929875152438 32.461039879801376 21.33053947891471 21.415433126040114 17 23.89478308329229 35.95931896540248 21.17184276034664 18.974055190958588 18 28.95276387261689 27.827111513473213 19.795771647672808 23.424352966237088 19 28.6054700240287 26.08678288304479 23.391707768392607 21.916039324533905 20 22.07505224903822 26.47062738781433 30.131388027315932 21.322932335831528 21 22.36258835744998 28.810186232877623 26.21038058598794 22.616844823684456 22 20.62198791170754 30.019037729318104 26.155576856511438 23.203397502462924 23 22.5083531901865 28.95461987992705 22.80254522703372 25.73448170285273 24 22.176887954100717 29.451817734055723 24.284820489610095 24.086473822233465 25 20.809094660614207 28.568576030670517 25.190692349675857 25.431636959039416 26 20.8264000745464 25.907535842174624 28.76825388373424 24.497810199544734 27 21.634325985945498 28.39780051808215 27.929768080458295 22.03810541551406 28 21.70897618508806 29.001876971818795 29.682112857922547 19.60703398517059 29 19.81070539529559 29.505065161539385 29.383261671170512 21.300967771994518 30 18.138776777398324 30.21022752472613 30.05499623009713 21.59599946777842 31 21.080271565495206 28.596246006389777 30.11182108626198 20.211661341853034 32 22.10914464094355 28.725181541656404 29.89596714611857 19.269706671281476 33 19.989350372736954 29.130058572949945 30.171725239616613 20.708865814696487 34 20.915728729175125 28.383064542967634 30.094315133685214 20.60689159417203 35 20.068289825747794 28.953954287083505 31.70684628798871 19.27090959917999 36 20.108225396360538 28.959944622675415 30.956723153312655 19.97510682765139 37 21.209781552428748 30.14070632712557 29.611560016506704 19.03795210393898 38 19.762117117716752 30.173320376459312 31.12312136419909 18.941441141624846 39 18.67187603998882 32.32185407542498 30.277818452895993 18.728451431690207 40 18.906830313760466 30.79365290664395 32.34182186073135 17.957694918864235 41 19.270244006336444 30.87751760493071 31.531129777292634 18.32110861144021 42 18.888193714141185 32.23066785585921 30.60063098201568 18.28050744798392 43 18.1114468657233 31.696196802491976 30.562026597090032 19.630329734694694 44 19.123147987912834 31.027275994728505 30.330400287536108 19.519175729822553 45 20.592776986461843 30.64655688822034 29.970980152021404 18.789685973296415 46 20.238016000851957 31.206986062485857 29.895768160700737 18.65922977596145 47 19.85396893012606 31.511161991986263 29.640846101622714 18.99402297626496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 9.5 11 19.0 12 54.5 13 90.0 14 150.5 15 211.0 16 319.0 17 427.0 18 615.5 19 804.0 20 1039.5 21 1275.0 22 1689.0 23 2103.0 24 2602.0 25 3101.0 26 3101.0 27 3664.5 28 4228.0 29 4716.0 30 5204.0 31 5699.0 32 6194.0 33 6785.0 34 7376.0 35 7813.5 36 8251.0 37 8948.5 38 9646.0 39 10492.0 40 11338.0 41 11770.5 42 12203.0 43 12203.0 44 12524.0 45 12845.0 46 13123.5 47 13402.0 48 13269.0 49 13136.0 50 12383.0 51 11630.0 52 10618.5 53 9607.0 54 8252.5 55 6898.0 56 5655.0 57 4412.0 58 4412.0 59 3557.0 60 2702.0 61 2110.5 62 1519.0 63 1163.0 64 807.0 65 611.5 66 416.0 67 316.5 68 217.0 69 159.0 70 101.0 71 75.0 72 49.0 73 37.0 74 25.0 75 25.0 76 15.0 77 5.0 78 3.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 19.047935996592162 6 1.5634775894889579 7 5.839911609270377 8 29.190239746542247 9 0.007987114122548955 10 1.2120445680968037 11 0.0 12 0.0 13 1.6080723100065228 14 4.289745876652335 15 0.001996778530637239 16 1.2120445680968037 17 0.0 18 0.01198067118382343 19 0.003327964217728731 20 0.0 21 0.0 22 0.009318299809640447 23 0.0 24 0.0 25 0.0 26 0.0 27 1.5901013032307878 28 0.0 29 0.0 30 39.97151262629624 31 0.0013311856870914923 32 6.655928435457462E-4 33 0.0013311856870914923 34 6.655928435457462E-4 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 150242.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.22883258453106 #Duplication Level Percentage of deduplicated Percentage of total 1 92.36387224767772 79.64428876904016 2 5.08523439152186 8.769876499992764 3 1.2350368953307203 3.19487369049575 4 0.5173466138999745 1.7844077823261972 5 0.2623539776173059 1.1311238606924239 6 0.1515062206635657 0.7838522722268176 7 0.09666536569531764 0.5834739144684811 8 0.05281444585451231 0.36433024077068177 9 0.03487700864935615 0.27066633658871164 >10 0.19032801787255435 2.7814068550068387 >50 0.007971852173712608 0.5026638141103517 >100 0.001992963043428152 0.189035964280816 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.005990335591911716 0.0 0.0 16 0.0 0.0 0.010649485496731939 0.0 0.0 17 0.0 0.0 0.021964563837009624 0.0 0.0 18 0.0 0.0 0.08253351259967252 0.0 0.0 19 0.0 0.0 0.10649485496731939 0.0 0.0 20 0.0 0.0 0.1484272041107014 0.0 0.0 21 0.0 0.0 0.1903595532540834 0.0 0.0 22 0.0 0.0 0.2349542737716484 0.0 0.0 23 0.0 0.0 0.29485762969076557 0.0 0.0 24 0.0 0.0 0.34810505717442525 0.0 0.0 25 0.0 0.0 0.40534604171935945 0.0 0.0 26 0.0 0.0 0.513837675217316 0.0 0.0 27 0.0 0.0 0.6296508299942759 0.0 0.0 28 0.0 0.0 0.786730741071072 0.0 0.0 29 0.0 0.0 0.9018783030044861 0.0 0.0 30 0.0 0.0 1.0243473862169035 0.0 0.0 31 0.0 0.0 1.235340317620905 0.0 0.0 32 0.0 0.0 1.4443364704942692 0.0 0.0 33 0.0 0.0 1.652001437680542 0.0 0.0 34 0.0 0.0 1.8696502975200011 0.0 0.0 35 0.0 0.0 2.098614235699738 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCGTG 15 0.006011576 54.427322 5 CTGCGTA 30 5.7798206E-7 54.427322 5 CTGCGAG 30 5.7798206E-7 54.427322 5 ACTGCGT 20 2.7397263E-4 54.42732 4 CTGAAGT 355 0.0 53.66074 5 CATTGAG 15 0.006465453 53.43573 3 CACTGTC 15 0.006465453 53.43573 3 TCACTGT 25 1.3993278E-5 53.43573 2 CAATGAT 15 0.006465453 53.43573 3 GCACTGC 730 0.0 52.703728 2 CTGATAC 135 0.0 52.411488 5 CTGACAT 120 0.0 52.159515 5 CACTGCT 200 0.0 52.099834 3 ACTGAAT 460 0.0 52.060913 4 CACTGCG 160 0.0 51.765858 3 GATCCGG 35 3.979585E-8 51.759315 7 TGACATA 35 3.979585E-8 51.759315 6 GATTAGG 45 9.458745E-11 51.759315 7 TGATACG 40 1.9444997E-9 51.75931 6 TGATACC 20 3.5122925E-4 51.75931 6 >>END_MODULE