FastQCFastQC Report
Thu 26 May 2016
SRR2099012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64149
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGGTCAGTCAGTGAGTCGTATACGGCCTCAGAGTTCTAC1370.2135652933015324No Hit
AGGTTGTCCATGCACCACTATAGTGAGTCGTATTACCGGCCG1090.16991691218881042No Hit
ACGATGTCTCCAACATTCCGACGATCGAATACGATGTCGGAA1010.15744594615660415No Hit
ACGATGTCCAGCACCAAAAATAATGAACTTAAAACAAAAATA1010.15744594615660415No Hit
ACGATGTCCGGAGATCGCGGCCGCCTCGGAGGCAATGCCATC960.14965159238647524No Hit
ACGATGTCTCTCCGATCGCTGACGATGTCTTTTTTTTTTTTT900.14029836786232053No Hit
AGGTTGTCCATGCACCACTATAGTNAGTCGTATTACCGGCCG840.13094514333816584No Hit
TCGAGGTCAGTCAGTGAGTCGTATNCGGCCTCAGAGTTCTAC830.12938627258414004No Hit
AGGTTGTCCATGCGGCCGACGATCCATGAGATCGTCACTGAC800.1247096603220627No Hit
ACGATGTCTCCAACATTCCGACGANCGAATACGATGTCGGAA800.1247096603220627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCG150.00798607650.6054522
CCGCCTC150.00798607650.6054521
GGCCGCC150.00798607650.6054519
TCCAGGC150.00798607650.6054521
GCCGCCT150.00798607650.6054520
GCCTCGG150.00829003350.1302823
CCTCGGA150.00829003350.1302824
GTATACG351.3921641E-436.1467521
ACGTTGT552.3646862E-1134.809291
ACGAGGT454.602043E-934.809291
AAGATGT900.034.809291
ACGGTGT1350.034.809291
ACGAAGT251.7469322E-434.809291
CCTATGT200.002466923334.8092881
CCGCTGT358.89504E-734.8092881
ACGATTT200.002466923334.8092881
GTCACTA251.797845E-434.6399046
GTCACGC301.2859646E-534.6399046
GTCGCTG650.034.6399046
GTCGCTA301.2859646E-534.6399046
Produced by FastQC (version 0.11.5)