##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099012_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64149 Sequences flagged as poor quality 0 Sequence length 42 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.994310121747805 28.0 23.0 31.0 12.0 35.0 2 28.43601614990101 30.0 27.0 32.0 19.0 35.0 3 28.332818905984507 30.0 26.0 32.0 19.0 35.0 4 22.10522377589674 24.0 15.0 29.0 7.0 32.0 5 26.528098645341316 28.0 24.0 31.0 13.0 35.0 6 26.663283917130432 29.0 24.0 32.0 10.0 37.0 7 27.19764922290293 30.0 24.0 31.0 13.0 37.0 8 28.69145271165568 30.0 27.0 32.0 17.0 37.0 9 25.843582908541052 26.0 26.0 28.0 22.0 31.0 10 26.07576111864565 27.0 26.0 28.0 20.0 31.0 11 25.91890754337558 27.0 26.0 27.0 20.0 31.0 12 28.087873544404435 30.0 26.0 31.0 18.0 35.0 13 30.97446569704906 31.0 31.0 31.0 30.0 34.0 14 30.8803878470436 31.0 31.0 33.0 28.0 34.0 15 31.090305382780713 31.0 31.0 34.0 30.0 34.0 16 34.94715428143853 35.0 35.0 37.0 32.0 37.0 17 34.938549314876305 35.0 35.0 37.0 32.0 37.0 18 34.56950225256824 35.0 35.0 37.0 32.0 37.0 19 34.45824564685342 35.0 35.0 37.0 31.0 37.0 20 34.328687898486336 35.0 35.0 37.0 30.0 37.0 21 35.59190322530359 37.0 35.0 39.0 30.0 39.0 22 35.36133065207564 37.0 34.0 39.0 30.0 39.0 23 35.570421986313114 37.0 35.0 39.0 30.0 39.0 24 35.65257447505027 37.0 35.0 39.0 30.0 39.0 25 22.459726574069744 31.0 2.0 37.0 2.0 39.0 26 29.15785125255265 33.0 17.0 38.0 17.0 40.0 27 33.49611061746871 34.0 32.0 38.0 27.0 40.0 28 35.709504434987295 37.0 34.0 39.0 31.0 40.0 29 36.648427878844565 38.0 36.0 39.0 32.0 40.0 30 37.184648240814354 38.0 36.0 40.0 33.0 40.0 31 37.62688428502393 39.0 37.0 40.0 33.0 41.0 32 37.80291197056852 39.0 37.0 40.0 34.0 41.0 33 37.89667804642317 39.0 37.0 40.0 34.0 41.0 34 37.9563983850099 39.0 37.0 40.0 34.0 41.0 35 37.92819841306957 39.0 37.0 40.0 34.0 41.0 36 37.93172146097367 39.0 37.0 40.0 34.0 41.0 37 37.8564591809693 39.0 37.0 40.0 33.0 41.0 38 37.67276185131491 39.0 37.0 40.0 33.0 41.0 39 37.56184819716597 39.0 37.0 40.0 33.0 41.0 40 37.3736301423249 39.0 36.0 40.0 33.0 41.0 41 37.17782038691172 39.0 36.0 40.0 32.0 41.0 42 37.01387394971083 39.0 36.0 40.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.125846373723327 1101 2 6.033464217432975 1101 3 5.4382744158240754 1101 4 4.1968490625185275 1101 5 7.24995914520078 1101 6 9.217234938591716 1101 7 7.01950018517487 1101 8 6.07578331514955 1101 9 5.838391308112996 1101 10 5.423084017087302 1101 11 3.354853071531995 1101 12 8.128214343708322 1101 13 0.25933697661765365 1101 14 0.9895797949098615 1101 15 1.4087493384195788 1101 16 1.0170721585843552 1101 17 0.253309515544224 1101 18 0.07082985532853314 1101 19 0.04530406512369467 1101 20 -0.5055179766996716 1101 21 -0.14846922984958155 1101 22 0.2270572524859631 1101 23 0.0025155339615494654 1101 24 -0.12959913618914243 1101 25 11.053516333717877 1101 26 6.470668860771099 1101 27 2.508479483902768 1101 28 1.044408273534934 1101 29 0.3832598378395531 1101 30 -0.09596869379919326 1101 31 0.23511350341533443 1101 32 0.23789807262572538 1101 33 0.2085954150526561 1101 34 -0.08548606651677915 1101 35 -0.10633438754471314 1101 36 -0.3374690744115796 1101 37 -0.3469597962472193 1101 38 -0.06977734336440733 1101 39 -0.17384672878712593 1101 40 -0.3977979720875453 1101 41 -0.10893108492271608 1101 42 -0.06340333956161714 1102 1 4.30052773431078 1102 2 3.2984768722128486 1102 3 2.2021918362919024 1102 4 0.10184174204048446 1102 5 3.8928446620038315 1102 6 5.122988039864005 1102 7 2.5065559910516804 1102 8 2.7997275206223193 1102 9 4.207831433412341 1102 10 3.6364560903147805 1102 11 3.657693870996294 1102 12 5.619492192736228 1102 13 0.08131883999143241 1102 14 1.4536413550229028 1102 15 1.8666367279030567 1102 16 0.9072990366558358 1102 17 0.5920603809495759 1102 18 0.2144133712009122 1102 19 0.2582789031084616 1102 20 0.02041013507097489 1102 21 0.013750293695117932 1102 22 0.5394316977753206 1102 23 0.4096249098482332 1102 24 0.12942094121498116 1102 25 11.819022695156544 1102 26 6.53479397852297 1102 27 2.823316787696932 1102 28 1.3581560178062375 1102 29 0.6446519771215833 1102 30 0.5171922772707305 1102 31 0.4725126340376278 1102 32 0.4053043534823928 1102 33 0.32350989597969004 1102 34 0.622773021658098 1102 35 0.5750375494878313 1102 36 0.21439282391889947 1102 37 0.09128147308963008 1102 38 0.35528706549280287 1102 39 0.044144622834167535 1102 40 0.4452033104148114 1102 41 0.22352076449107727 1102 42 0.37700569924258787 1103 1 1.5307722248207831 1103 2 -0.12600746304641675 1103 3 -0.7290038590285199 1103 4 -1.288108124908458 1103 5 1.2026110508069578 1103 6 1.4042770914525313 1103 7 -1.5659531608029482 1103 8 -0.6322253160850728 1103 9 2.4232124298986157 1103 10 1.9012791544586243 1103 11 -1.4227689408650974 1103 12 2.2188835392717543 1103 13 -0.16817027334454693 1103 14 1.5513934537012126 1103 15 1.860006227601673 1103 16 0.9315569645877417 1103 17 0.6743021075259747 1103 18 0.7532334067792092 1103 19 -0.04991439582271795 1103 20 0.2826456869020859 1103 21 0.5158747001109703 1103 22 0.6333025279845259 1103 23 -0.04836476950605828 1103 24 0.272028153905552 1103 25 11.922508485891488 1103 26 6.548363716390334 1103 27 2.488454469815167 1103 28 1.0746175947185677 1103 29 0.5776412889315168 1103 30 0.16318824898451822 1103 31 0.15902669628493982 1103 32 0.20059684528615662 1103 33 0.24206206522651286 1103 34 0.2286331489754616 1103 35 0.16766607975143444 1103 36 0.40660382378658255 1103 37 0.11174634319944943 1103 38 0.3647256047244909 1103 39 0.10240775519424261 1103 40 0.28066105016399945 1103 41 0.11025829371085649 1103 42 0.0985834936823764 1104 1 1.4938065304527797 1104 2 1.0928514619214802 1104 3 -1.1681434664400072 1104 4 -1.6006654538389284 1104 5 1.0665809645842472 1104 6 0.5638808338851149 1104 7 -1.0835187450994077 1104 8 -0.4128160333846509 1104 9 2.194171886878987 1104 10 0.9916295488816651 1104 11 1.1076383238569711 1104 12 3.362435181172323 1104 13 0.17621607778368542 1104 14 1.8491369869978556 1104 15 1.6518334152736571 1104 16 0.5205773278238439 1104 17 0.34078532161512953 1104 18 -0.11495591628077761 1104 19 -0.003638483146147564 1104 20 -0.0286733371302077 1104 21 -0.23646432025226716 1104 22 0.36477381765614325 1104 23 -0.21631560421528917 1104 24 0.16360768059790587 1104 25 11.761253670261329 1104 26 6.680908203640378 1104 27 2.3963612759057895 1104 28 0.431983215705543 1104 29 0.03590582754828375 1104 30 0.03270760323144373 1104 31 -0.16504228188419745 1104 32 -0.015831333029751704 1104 33 0.23348565722798043 1104 34 0.30389456889841426 1104 35 0.02539860589342169 1104 36 0.11142862657506214 1104 37 0.07514755398646145 1104 38 0.16678027359258607 1104 39 -0.2846313202047668 1104 40 -0.2506634774456131 1104 41 -0.24628179067732958 1104 42 -0.21443246669186067 1105 1 -0.5272112412043803 1105 2 1.0020258340114516 1105 3 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17 0.8770172056730985 2208 18 1.3560585764728472 2208 19 0.7391151400422586 2208 20 0.1452397063480575 2208 21 0.2128110420665763 2208 22 0.7597013538880333 2208 23 0.921365555205 2208 24 1.4534627530616717 2208 25 -21.731499952927077 2208 26 -12.732135274620493 2208 27 -3.1398706081521794 2208 28 -0.9013501176277856 2208 29 -0.9785869260749038 2208 30 -0.3622199330004392 2208 31 -1.0563466297102835 2208 32 -0.5339472750587362 2208 33 0.6573987007062456 2208 34 0.8727351486085553 2208 35 0.23554353342965584 2208 36 0.2998344236765149 2208 37 0.4447127713777661 2208 38 0.021852737360703145 2208 39 0.9218904189256634 2208 40 0.7312205805254024 2208 41 0.9203848759893347 2208 42 0.4775965188153819 2209 1 -5.692369612245102 2209 2 -4.115621224812077 2209 3 -4.362402106350821 2209 4 -0.6667189655446144 2209 5 -2.144678790153769 2209 6 -4.145706680050228 2209 7 -3.1389813983881503 2209 8 -1.3743544949231108 2209 9 -2.23615141300953 2209 10 0.8227332278114297 2209 11 -1.5024508623191686 2209 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 24 5.0 25 45.0 26 298.0 27 957.0 28 2137.0 29 4076.0 30 6417.0 31 8939.0 32 10803.0 33 11177.0 34 9140.0 35 6229.0 36 2675.0 37 1225.0 38 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.7310022503029254 92.59131036870349 2.9332619950587757 2.7444253859348198 2 3.559359158896051 2.659740909944302 0.8464234307528632 92.93447650040679 3 95.33118209169278 1.8192021699480896 1.8519384557826308 0.9976772825765016 4 3.2814229372242742 86.38170509283076 4.765467895056821 5.571404074888151 5 2.6033110072033363 1.9129912801718691 93.84557058005814 1.6381271325666624 6 94.68888853242649 3.1728717858231343 1.2816605444250173 0.8565791373253556 7 1.1021388040157136 2.5144977240132196 94.33497536945814 2.0483881025129387 8 1.6099759051225084 2.91954814281691 0.7885596269987796 94.68191632506179 9 31.80496043536734 16.857973915491193 25.166546773840427 26.170518875301035 10 26.34809088779574 26.513625361130632 16.70805028500039 30.430233466073243 11 30.98179199348707 14.160913061857123 29.386438714323738 25.470856230332068 12 24.278318167555696 28.893944148101664 16.05898964543458 30.76874803890806 13 29.75303252362094 30.29405344725436 17.647884249586827 22.305029779537872 14 28.407898651717062 27.047088116005575 20.85375593103556 23.691257301241798 15 22.461620069895158 28.310659011482777 28.939403394907636 20.288317523714426 16 24.441534552370264 28.19529532806435 25.646541645232197 21.716628474333195 17 20.41783598378547 29.792641097599002 25.8933582787652 23.89616463985033 18 23.397091147172986 28.670750908042216 20.195170618404028 27.73698732638077 19 23.595067732934265 28.64269123446975 23.302000031177414 24.460241001418574 20 22.93722427473538 29.800932204710907 24.142231367597315 23.1196121529564 21 21.962930053469268 28.167235654491883 24.402562783519617 25.46727150851923 22 22.089720857981202 29.506833409172927 24.8638809998274 23.539564733018466 23 22.924753308703174 30.109588614008015 24.75642644468347 22.20923163260534 24 20.115668209948716 29.10411697766138 27.857020374440754 22.92319443794915 25 19.4548143974079 29.792679204526777 27.808691977178277 22.943814420887044 26 21.80426513687099 29.823221300742038 26.365592068341957 22.00692149404502 27 24.001932999734994 29.983320082931925 26.040935946000715 19.973810971332366 28 21.919965080206403 31.502642368310283 26.17113816702262 20.40625438446069 29 19.7945408346194 33.155622067374395 26.179675443109012 20.870161654897192 30 20.41341252396764 33.038706760822464 26.85934309186425 19.68853762334565 31 19.460942493257885 33.50325024552214 28.210884035604604 18.824923225615365 32 19.78518761009525 33.517280082308375 27.97393568099269 18.72359662660369 33 19.314408642379462 33.77917036898471 28.291945314813947 18.614475673821886 34 19.774275514817067 35.54537093329592 27.05420193611748 17.626151615769537 35 19.85689566478043 35.25853871455518 27.635660727369093 17.248904893295297 36 19.822600508191865 35.199301625902194 27.247501909616673 17.730595956289264 37 19.710361813902008 34.10497435657609 28.215560647866685 17.969103181655207 38 20.121903692964814 33.09638497872142 27.78531231975557 18.9963990085582 39 21.013577764267566 32.966998706137275 27.641896210385198 18.377527319209964 40 19.467177976273987 33.747992953904195 28.111116307346958 18.673712762474864 41 18.874807089744188 34.56640009976773 27.967700197976587 18.5910926125115 42 19.5466803847293 34.267096914994774 27.934963912142045 18.251258788133875 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 4.0 9 6.0 10 6.0 11 18.0 12 30.0 13 69.0 14 108.0 15 172.5 16 237.0 17 237.0 18 350.0 19 463.0 20 624.0 21 785.0 22 785.0 23 1004.0 24 1223.0 25 1438.5 26 1654.0 27 1836.5 28 2019.0 29 2019.0 30 2209.0 31 2399.0 32 2579.0 33 2759.0 34 2759.0 35 3158.5 36 3558.0 37 3811.5 38 4065.0 39 4154.0 40 4243.0 41 4243.0 42 4316.5 43 4390.0 44 4544.0 45 4698.0 46 5175.5 47 5653.0 48 5653.0 49 5793.5 50 5934.0 51 5963.0 52 5992.0 53 5992.0 54 5329.0 55 4666.0 56 4005.5 57 3345.0 58 2803.0 59 2261.0 60 2261.0 61 1877.5 62 1494.0 63 1237.0 64 980.0 65 797.0 66 614.0 67 614.0 68 450.5 69 287.0 70 205.0 71 123.0 72 123.0 73 94.0 74 65.0 75 60.0 76 55.0 77 44.0 78 33.0 79 33.0 80 19.5 81 6.0 82 4.0 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.9384401939235218 2 0.3632168856880076 3 0.0 4 0.0 5 1.3172457871517873 6 2.8184383232786168 7 0.0015588707540257838 8 0.3663346271960592 9 0.31645076306723413 10 0.17771126595893935 11 0.4302483281111163 12 0.6375781383965455 13 0.017147578294283622 14 0.4520725186674773 15 0.08262014996336653 16 0.0 17 0.014029836786232054 18 0.0 19 0.0 20 0.0 21 0.0 22 0.6516079751827777 23 0.0 24 0.0 25 33.60613571528784 26 0.0015588707540257838 27 0.0 28 0.0031177415080515677 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 64149.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.53899515191196 #Duplication Level Percentage of deduplicated Percentage of total 1 88.3398847227031 67.61446008511435 2 7.040876596264689 10.77803239333427 3 2.014297643536528 4.6251695271945 4 0.7983869325240841 2.4443093423124287 5 0.462331208374916 1.7693183058192647 6 0.2953216969795719 1.3562175560024319 7 0.22607385079125847 1.211242575878034 8 0.14664249781054603 0.8979095543188514 9 0.11201857471638933 0.771641023242763 >10 0.5315790545632294 6.502049915041544 >50 0.024440416301757674 1.3312756239380192 >100 0.008146805433919225 0.6983740978035511 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGAGGTCAGTCAGTGAGTCGTATACGGCCTCAGAGTTCTAC 137 0.2135652933015324 No Hit AGGTTGTCCATGCACCACTATAGTGAGTCGTATTACCGGCCG 109 0.16991691218881042 No Hit ACGATGTCTCCAACATTCCGACGATCGAATACGATGTCGGAA 101 0.15744594615660415 No Hit ACGATGTCCAGCACCAAAAATAATGAACTTAAAACAAAAATA 101 0.15744594615660415 No Hit ACGATGTCCGGAGATCGCGGCCGCCTCGGAGGCAATGCCATC 96 0.14965159238647524 No Hit ACGATGTCTCTCCGATCGCTGACGATGTCTTTTTTTTTTTTT 90 0.14029836786232053 No Hit AGGTTGTCCATGCACCACTATAGTNAGTCGTATTACCGGCCG 84 0.13094514333816584 No Hit TCGAGGTCAGTCAGTGAGTCGTATNCGGCCTCAGAGTTCTAC 83 0.12938627258414004 No Hit AGGTTGTCCATGCGGCCGACGATCCATGAGATCGTCACTGAC 80 0.1247096603220627 No Hit ACGATGTCTCCAACATTCCGACGANCGAATACGATGTCGGAA 80 0.1247096603220627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0015588707540257838 0.0 0.0 11 0.0 0.0 0.006235483016103135 0.0 0.0 12 0.0 0.0 0.015588707540257837 0.0 0.0 13 0.0 0.0 0.057678217898954 0.0 0.0 14 0.0 0.0 0.08417902071739232 0.0 0.0 15 0.0 0.0 0.12938627258414004 0.0 0.0 16 0.0 0.0 0.1621225584186815 0.0 0.0 17 0.0 0.0 0.21824190556360973 0.0 0.0 18 0.0 0.0 0.24318383762802226 0.0 0.0 19 0.0 0.0 0.2759201234625637 0.0 0.0 20 0.0 0.0 0.3647757564420334 0.0 0.0 21 0.0 0.0 0.43336606961916785 0.0 0.0 22 0.0 0.0 0.634460396888494 0.0 0.0 23 0.0 0.0 0.7825531185209434 0.0 0.0 24 0.0 0.0 0.8636143977302841 0.0 0.0 25 0.0 0.0 0.9306458401533929 0.0 0.0 26 0.0 0.0 1.1145925891284354 0.0 0.0 27 0.0 0.0 1.4029836786232053 0.0 0.0 28 0.0 0.0 1.7116400879203106 0.0 0.0 29 0.0 0.0 1.9111755444356109 0.0 0.0 30 0.0 0.0 2.0920045519026016 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCTCG 15 0.007986076 50.60545 22 CCGCCTC 15 0.007986076 50.60545 21 GGCCGCC 15 0.007986076 50.60545 19 TCCAGGC 15 0.007986076 50.60545 21 GCCGCCT 15 0.007986076 50.60545 20 GCCTCGG 15 0.008290033 50.13028 23 CCTCGGA 15 0.008290033 50.13028 24 GTATACG 35 1.3921641E-4 36.14675 21 ACGTTGT 55 2.3646862E-11 34.80929 1 ACGAGGT 45 4.602043E-9 34.80929 1 AAGATGT 90 0.0 34.80929 1 ACGGTGT 135 0.0 34.80929 1 ACGAAGT 25 1.7469322E-4 34.80929 1 CCTATGT 20 0.0024669233 34.809288 1 CCGCTGT 35 8.89504E-7 34.809288 1 ACGATTT 20 0.0024669233 34.809288 1 GTCACTA 25 1.797845E-4 34.639904 6 GTCACGC 30 1.2859646E-5 34.639904 6 GTCGCTG 65 0.0 34.639904 6 GTCGCTA 30 1.2859646E-5 34.639904 6 >>END_MODULE