FastQCFastQC Report
Thu 26 May 2016
SRR2099011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74177
Sequences flagged as poor quality0
Sequence length42
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACACCGGTAGGTTGAGGGTAGAGGATGGACATCAGTAA3400.458363104466344No Hit
TGCTACACCGGTAGGTTGAGGGTANAGGATGGACATCAGTAA2130.2871510036803861No Hit
CGCTATAGGTACGTACGATCGTCGGACTGTAGAACTCTGAAC1410.19008587567574856Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCTATAGGTACGTACGATCGTCGNACTGTAGAACTCTGAAC930.1253757903393235Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGCTACAGGCCCGACGATCTCCCTGCTACAGTTTTTTTTTTT910.12267953678363913No Hit
CGATACAGGTCAGTGAAACCCCGTCTCTACTAAAAATACAAA820.11054639578305944No Hit
TTCGACAGTAACTGAGATCATGCCATTGCACTCTAGCCTGGG800.10785014222737506No Hit
TGCTACAGGTTCGGCGGAGTCCTTGGATCTTGGGGATGGCGC780.10515388867169068No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG150.00796557250.65025320
ACACAAT150.00836078350.03444723
CCAGTCA150.00836078350.03444725
TGAGCGT250.001167390540.52020622
CATGCTC250.001239922240.02755724
ATGCTCG250.001239922240.02755725
GAGGATG651.10958354E-1038.48803725
TAGAGGA602.1882443E-937.52583323
CGCGACA200.002523833434.6554371
TGCGGCA251.7959195E-434.6554371
CGCTATA552.5465852E-1134.6554371
TGCTCTA200.002523833434.6554371
AGAGGAT654.78758E-934.63923324
CAGGTCA1050.034.6311386
CAGCGTA251.8033518E-434.6311386
CAGCGGC251.8033518E-434.6311386
CAGCCGG503.5288394E-1034.6311386
CAGGCCG503.5288394E-1034.6311386
CAGGCAT251.8033518E-434.6311386
CAGGCAC251.8033518E-434.6311386
Produced by FastQC (version 0.11.5)