##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74177
Sequences flagged as poor quality	0
Sequence length	42
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.27337314801084	30.0	25.0	31.0	16.0	35.0
2	27.48513690227429	30.0	25.0	31.0	16.0	35.0
3	28.343961066098657	30.0	27.0	31.0	17.0	35.0
4	28.424268978254716	30.0	27.0	32.0	18.0	35.0
5	25.20571066503094	28.0	21.0	31.0	11.0	34.0
6	27.789031640535477	30.0	26.0	31.0	17.0	37.0
7	24.721706189250035	27.0	18.0	31.0	10.0	34.0
8	26.46786739825013	28.0	24.0	31.0	13.0	35.0
9	25.42637205602815	27.0	26.0	27.0	18.0	31.0
10	25.582822168596735	27.0	26.0	27.0	18.0	31.0
11	25.120684309152434	27.0	25.0	27.0	16.0	31.0
12	27.28616687113256	30.0	25.0	31.0	17.0	35.0
13	30.806139369346294	31.0	31.0	31.0	28.0	34.0
14	30.744017687423327	31.0	31.0	33.0	27.0	34.0
15	30.99572643811424	31.0	31.0	34.0	28.0	34.0
16	34.87695646898634	35.0	35.0	37.0	32.0	37.0
17	34.961686236973726	35.0	35.0	37.0	32.0	37.0
18	34.57219892958734	35.0	35.0	37.0	32.0	37.0
19	34.49813284441269	35.0	35.0	37.0	31.0	37.0
20	34.52010731089152	35.0	35.0	37.0	32.0	37.0
21	36.0064979710692	37.0	35.0	39.0	32.0	39.0
22	35.87544656699516	37.0	35.0	39.0	32.0	39.0
23	36.09705164673686	37.0	35.0	39.0	32.0	39.0
24	36.29355460587514	37.0	35.0	39.0	32.0	39.0
25	23.037019561319546	32.0	2.0	37.0	2.0	39.0
26	29.65138789651779	34.0	17.0	38.0	17.0	40.0
27	34.108268061528506	36.0	32.0	39.0	27.0	40.0
28	36.177076452269574	37.0	34.0	39.0	32.0	40.0
29	36.9615783868315	38.0	36.0	39.0	32.0	40.0
30	37.35374846650579	38.0	36.0	40.0	33.0	40.0
31	37.7223532901034	39.0	37.0	40.0	33.0	41.0
32	37.84759426776494	39.0	37.0	40.0	33.0	41.0
33	37.87996279170093	39.0	37.0	40.0	34.0	41.0
34	37.91102363266241	39.0	37.0	40.0	34.0	41.0
35	37.92271189182631	39.0	37.0	40.0	34.0	41.0
36	37.93422489450908	39.0	37.0	40.0	33.0	41.0
37	37.8670477371692	39.0	37.0	40.0	33.0	41.0
38	37.67104358493873	39.0	37.0	40.0	33.0	41.0
39	37.57653989781199	39.0	37.0	40.0	33.0	41.0
40	37.44778030926028	39.0	36.0	40.0	33.0	41.0
41	37.36338757296736	39.0	36.0	40.0	33.0	41.0
42	37.303989107135635	39.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.776655735480258
1101	2	5.83339124036274
1101	3	5.185389448575023
1101	4	5.270680586720058
1101	5	6.803106023084492
1101	6	7.980724301442997
1101	7	5.481332465047316
1101	8	6.5636985329043505
1101	9	5.897235975499406
1101	10	5.357068904749649
1101	11	3.6270187457132153
1101	12	7.656181661674392
1101	13	0.4375290793723181
1101	14	1.3030139820966973
1101	15	1.7124800173157233
1101	16	1.1451967935872318
1101	17	0.7281565089504696
1101	18	0.4759622558812211
1101	19	0.35508303220607473
1101	20	0.2916667665427255
1101	21	0.02064010107959291
1101	22	1.065379494263496
1101	23	0.6661892013299493
1101	24	0.23055886505686374
1101	25	11.706845318713508
1101	26	7.108779540191485
1101	27	2.9724909069505685
1101	28	1.4028232925454205
1101	29	0.9236402148431964
1101	30	0.39843355001560354
1101	31	0.3883110481105234
1101	32	0.38248969635478147
1101	33	0.6152608109694739
1101	34	0.5206140381961291
1101	35	0.6240667188100986
1101	36	0.5873765385711707
1101	37	0.5413704080968671
1101	38	0.3558188583244757
1101	39	0.43861488855780806
1101	40	0.5920400386652744
1101	41	0.18227329009919657
1101	42	0.42069142543066107
1102	1	3.867589794554938
1102	2	2.679554049447823
1102	3	1.8526636739376876
1102	4	1.2769975326140468
1102	5	4.062749977043193
1102	6	5.393989055548182
1102	7	2.446110706123278
1102	8	3.6609162469855647
1102	9	4.3980890544903595
1102	10	3.7838081455509602
1102	11	3.831699444488944
1102	12	5.824733424843018
1102	13	-0.3836156277927145
1102	14	1.1177834843147032
1102	15	1.7421421980353031
1102	16	1.018025603521231
1102	17	0.5576239379784624
1102	18	0.5058408273692407
1102	19	0.09475661505460664
1102	20	0.2896526678518896
1102	21	0.2602845544016077
1102	22	1.0458044524418213
1102	23	0.8418236008039557
1102	24	0.11793579280731592
1102	25	12.0702736143033
1102	26	6.99837031195748
1102	27	2.965782793129023
1102	28	1.4119117048197722
1102	29	0.6511210101624982
1102	30	0.5204529752484319
1102	31	0.05473635584316128
1102	32	-0.2763119981513924
1102	33	-0.11517455061117232
1102	34	0.3840381724249866
1102	35	0.22232061176325146
1102	36	0.02061582514745197
1102	37	-0.04581426477854933
1102	38	-0.2577238746909316
1102	39	0.18325713635852736
1102	40	-0.10529585806639119
1102	41	0.1923770744408273
1102	42	0.21761701197448247
1103	1	0.9186295546272696
1103	2	-2.2131290387388276
1103	3	-0.34332503534848513
1103	4	0.011224714125500412
1103	5	0.09528796191540323
1103	6	1.3987633515324802
1103	7	-1.656167283600407
1103	8	-0.3809939124516646
1103	9	1.9174949688838154
1103	10	1.2527476564337086
1103	11	-1.458441027868652
1103	12	1.2968484489901506
1103	13	-0.9950783485901198
1103	14	0.9781160311204466
1103	15	1.4102238792931594
1103	16	0.9606237908324076
1103	17	0.4086173884823552
1103	18	-0.022419035466228365
1103	19	-0.09564186775572381
1103	20	0.1104015597300787
1103	21	-0.37834644654663663
1103	22	0.16201941704807865
1103	23	0.22287416644225289
1103	24	-0.17475170581442256
1103	25	11.710635711024135
1103	26	6.720259301306278
1103	27	2.822443652722825
1103	28	1.2349099185935586
1103	29	0.5874012492080851
1103	30	0.39336628814675123
1103	31	0.19638850482765946
1103	32	-0.5309043636882507
1103	33	-0.036415206001827016
1103	34	0.40093013881747197
1103	35	0.09600575080221319
1103	36	0.30610328848570845
1103	37	0.15703267107166852
1103	38	0.3597701160912621
1103	39	0.21546433859902692
1103	40	0.3577998263228679
1103	41	0.8756637306694657
1103	42	0.5942350903479152
1104	1	1.2412083191150387
1104	2	1.1502836615246927
1104	3	-0.6746955724161836
1104	4	-0.8332985012845882
1104	5	0.7147581235254385
1104	6	1.3095513462154393
1104	7	-0.939486236050211
1104	8	0.18220424057648188
1104	9	2.063282147354464
1104	10	0.8932644354267367
1104	11	0.7521650680712746
1104	12	2.6303761185874066
1104	13	0.14080934059623118
1104	14	1.8883210170116307
1104	15	1.652067586296262
1104	16	0.49736500488991453
1104	17	0.16482643211472947
1104	18	0.03292015904882106
1104	19	-0.17630696418537894
1104	20	-0.04273152951690662
1104	21	-0.22834994459938684
1104	22	0.735909951317339
1104	23	0.4154652907164049
1104	24	-0.181572908678163
1104	25	11.572334365439847
1104	26	6.608053429241728
1104	27	2.2078124640844976
1104	28	0.5304603954947567
1104	29	-0.13528478889737272
1104	30	-0.11060983181313588
1104	31	-0.3897102335413152
1104	32	-0.4235374645954124
1104	33	-0.23145496720142944
1104	34	0.14438295008586977
1104	35	0.04110964296890529
1104	36	-0.12746868977972525
1104	37	-0.15570490501097822
1104	38	0.11139671062030487
1104	39	0.051417324770056894
1104	40	-0.2517008849478586
1104	41	0.0915402260870124
1104	42	-0.029875821244630174
1105	1	0.5391815619265401
1105	2	1.4793321838889106
1105	3	-1.6464158200200174
1105	4	-1.0524728223708486
1105	5	-0.7028986256758394
1105	6	-1.4150767488324831
1105	7	-2.1348112160821593
1105	8	-4.664553447123197
1105	9	1.7790369396867334
1105	10	0.6851723827110803
1105	11	1.5606340177517843
1105	12	2.717411861398908
1105	13	0.2632758941106097
1105	14	1.801023193529204
1105	15	1.8705055854975399
1105	16	0.9684362908324076
1105	17	0.4100715399422086
1105	18	0.11221828205201945
1105	19	-0.1474780903834585
1105	20	-0.1171874001239388
1105	21	-0.5870884629699944
1105	22	0.37332758310647307
1105	23	0.06040838177072061
1105	24	-0.07324623136187114
1105	25	11.62341513620662
1105	26	6.688324994736938
1105	27	2.842659209292165
1105	28	1.1174087780826127
1105	29	0.5979794882591776
1105	30	-0.07854862973645993
1105	31	0.014505521251017228
1105	32	-0.006155276097011608
1105	33	-0.28632966768066126
1105	34	0.11300814976637952
1105	35	-0.3336532364240625
1105	36	-0.47206733195224615
1105	37	-0.17400063184803827
1105	38	-0.5322234970474256
1105	39	-0.9512783621308998
1105	40	-0.7808292795165457
1105	41	-0.5902064791845518
1105	42	-0.8889138603820115
1106	1	-4.637768224398247
1106	2	-4.454628820384595
1106	3	-2.387401396943094
1106	4	-6.193965877926406
1106	5	-0.5099899718296861
1106	6	0.8836345225350399
1106	7	-0.8103253720650656
1106	8	-3.5319078808838817
1106	9	-0.7372557953560026
1106	10	0.4702284724546715
1106	11	0.3856540498030654
1106	12	2.7257585494330954
1106	13	0.28781515693112425
1106	14	1.8685645931018549
1106	15	1.7657045705402759
1106	16	0.7683160985247142
1106	17	0.34520108054049814
1106	18	0.19521774786398538
1106	19	0.00483227286440524
1106	20	0.24893172380768647
1106	21	0.171625479337699
1106	22	0.759311824559461
1106	23	0.5976012236510613
1106	24	0.34315334128770303
1106	25	11.946832604155336
1106	26	6.550504481916427
1106	27	2.6460446259588295
1106	28	1.299199643467226
1106	29	0.6121688579172968
1106	30	0.6349596501780681
1106	31	0.21479264731939196
1106	32	0.08292057860384006
1106	33	0.46236824898600304
1106	34	0.4830001369458685
1106	35	0.11269425289217594
1106	36	0.36059826633835
1106	37	0.1941216972117914
1106	38	0.319405776456847
1106	39	0.08264257804003705
1106	40	0.49608044270567575
1106	41	0.38598876654194214
1106	42	0.273379141754738
1107	1	3.263310660287196
1107	2	2.13980861831514
1107	3	0.27968561440621187
1107	4	-3.0482463879446193
1107	5	-21.16383612567584
1107	6	-23.137732998832483
1107	7	-8.363358859524782
1107	8	-7.495688180729754
1107	9	2.120737759358864
1107	10	2.6345832433668193
1107	11	2.4478650628337526
1107	12	3.865593213857924
1107	13	0.5208962834548707
1107	14	1.9321066976275638
1107	15	1.8585306879565557
1107	16	1.3540664137832295
1107	17	0.42345524895860365
1107	18	0.3523566017241535
1107	19	-0.2764483772687072
1107	20	0.7233607556137684
1107	21	-0.16822319657655527
1107	22	1.8006457183523779
1107	23	0.6819503899674402
1107	24	0.3442742604414093
1107	25	12.401015033747601
1107	26	7.038734830802511
1107	27	3.1023287789642993
1107	28	-0.08667677519607508
1107	29	0.15644516448868728
1107	30	-0.3457745518676063
1107	31	-0.25143966317521205
1107	32	-0.722599948228158
1107	33	-0.4747902209593491
1107	34	-0.24188426826641063
1107	35	-0.8233432978994699
1107	36	-0.09834806965716325
1107	37	0.5945700648732739
1107	38	-0.04426243147365483
1107	39	-0.9640857391800779
1107	40	-1.0458139106640871
1107	41	-0.04416395869274936
1107	42	0.11960304535569577
1113	1	-9.518110104740126
1113	2	-5.7836886494444215
1113	3	-3.3104783200200174
1113	4	1.0282563442958192
1113	5	4.169497207657493
1113	6	-23.137732998832483
1113	7	-6.904342466082159
1113	8	-1.0148138637898647
1113	9	-20.502213060313267
1113	10	-21.11951511728892
1113	11	-11.563063898914882
1113	12	-21.462275638601092
1113	13	0.9416613107772775
1113	14	2.718991943529204
1113	15	1.5306618354975399
1113	16	-2.3727095425009246
1113	17	0.22126945660887287
1113	18	-0.27059421794798055
1113	19	1.6415844096165415
1113	20	1.2916667665427255
1113	21	0.9793177870300056
1113	22	3.1011921664398088
1113	23	3.2557208817707206
1113	24	2.1038371019714646
1113	25	14.72888388620662
1113	26	8.896658328070274
1113	27	5.812711292625501
1113	28	3.6838150280826127
1113	29	2.9925107382591776
1113	30	1.60504512026354
1113	31	2.3387242712510172
1113	32	2.561553057236324
1113	33	0.962368248986003
1113	34	1.8291539830997152
1113	35	0.3733780135759375
1113	36	2.5956410013810896
1113	37	0.6983952014852974
1113	38	0.8792348362859101
1113	39	2.6424716378691
1113	40	2.10719155381679
1113	41	1.2235956041487839
1113	42	2.3545757229513242
1114	1	-8.393110104740128
1114	2	-0.4920219827777572
1114	3	-1.1438116533533496
1114	4	-6.6800769890375165
1114	5	-0.9138361256758394
1114	6	-0.7210663321658153
1114	7	-0.07100913274882714
1114	8	2.193519469543471
1114	9	-5.1688797269799345
1114	10	0.17215154937774813
1114	11	1.7702694344184522
1114	12	-21.462275638601092
1114	13	-0.26667202255605815
1114	14	-27.281008056470796
1114	15	-13.96933816450246
1114	16	-6.414376209167592
1114	17	-2.403730543391127
1114	18	-1.4789275512813163
1114	19	-1.1917489237167942
1114	20	0.0416667665427255
1114	21	-0.3956822129699944
1114	22	1.0595254997731374
1114	23	0.5890542151040563
1114	24	-0.27116289802853544
1114	25	11.35388388620662
1114	26	5.854991661403602
1114	27	2.6460446259588295
1114	28	2.3088150280826127
1114	29	0.5758440715925133
1114	30	0.8550451202635401
1114	31	0.3803909379176815
1114	32	-0.5217802760970116
1114	33	-0.8709650843473256
1114	34	-0.3375126835669562
1114	35	-0.4182886530907268
1114	36	-0.9460256652855819
1114	37	-0.30160479851470257
1114	38	-0.49576516371408985
1114	39	-1.8575283621308998
1114	40	-1.26780844618321
1114	41	-0.23473772918455182
1114	42	0.7295757229513242
1115	1	-7.85144343807346
1115	2	-2.7230825888383627
1115	3	-0.12866013820183397
1115	4	-0.45659214055266517
1115	5	2.108891147051434
1115	6	3.4986306375311536
1115	7	4.004748443008751
1115	8	3.3791255301495298
1115	9	-20.502213060313267
1115	10	-21.11951511728892
1115	11	-8.623669959520942
1115	12	-21.462275638601092
1115	13	0.002267371383336325
1115	14	0.6280828526201141
1115	15	-27.46933816450246
1115	16	-15.039376209167592
1115	17	-5.081760846421428
1115	18	-2.8160487634025273
1115	19	-2.267506499474365
1115	20	-1.829545354669392
1115	21	0.43386324157545886
1115	22	-1.1412320759844334
1115	23	0.2557208817707206
1115	24	-0.22949623136187114
1115	25	11.819792977115714
1115	26	8.290597722009664
1115	27	2.5702870502012587
1115	28	1.0474513917189796
1115	29	-0.6438528981044556
1115	30	0.4232269384453602
1115	31	-0.11582118329443603
1115	32	0.41003790572116827
1115	33	-0.4315711449533879
1115	34	-0.8678157138699873
1115	35	-0.8084401682422424
1115	36	-0.3740559683158793
1115	37	0.0014255045155948665
1115	38	-0.18137122432015218
1115	39	1.187926183323647
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2207	2	-0.16258553193842573
2207	3	-4.519111413545197
2207	4	-2.2211441353204897
2207	5	0.059185457057974844
2207	6	0.8334900227502509
2207	7	-2.3575798761541016
2207	8	-0.8661328086339886
2207	9	0.9654128389673104
2207	10	0.7078230122074842
2207	11	1.0028833432913515
2207	12	1.3578682462909946
2207	13	0.13830399662859705
2207	14	1.992373238493233
2207	15	1.5450503247061746
2207	16	0.9462353016237728
2207	17	0.30999847339544573
2207	18	0.2617798827714424
2207	19	-0.10661702923238181
2207	20	0.1501799559911703
2207	21	-0.027876457574308233
2207	22	0.7534703438978383
2207	23	-0.13996257146668967
2207	24	-0.17913651913165296
2207	25	6.815214821458419
2207	26	4.167641541499528
2207	27	1.839090189507992
2207	28	0.5471243806006001
2207	29	-0.15856839843146986
2207	30	0.20216742242181596
2207	31	-0.04257069277775827
2207	32	-0.10990977249988987
2207	33	0.2309533808804929
2207	34	0.10733144113328308
2207	35	0.17193916465507186
2207	36	-0.32042614490188726
2207	37	-0.46947050594875606
2207	38	-0.7562567704287204
2207	39	-0.08414706716687448
2207	40	-0.19736480109927612
2207	41	-0.2320398874579297
2207	42	-0.3456640852021522
2208	1	0.5432934040318038
2208	2	-23.45035531611109
2208	3	-10.310478320020017
2208	4	-2.1120945328971636
2208	5	3.1782691374820544
2208	6	4.125424895904359
2208	7	2.9377627970757345
2208	8	-23.348147197123197
2208	9	2.866207992318312
2208	10	3.3804848827110803
2208	11	-20.896397232248216
2208	12	-6.857012480706356
2208	13	0.16973148621587342
2208	14	2.087412996160783
2208	15	2.056977624971225
2208	16	0.9869395803060925
2208	17	0.37039226362642097
2208	18	0.6504584136309646
2208	19	0.9836896727744389
2208	20	0.9671053630339586
2208	21	0.9003704186089507
2208	22	1.9520693594222607
2208	23	0.8346682501917755
2208	24	0.3757669265328687
2208	25	-22.27111611379338
2208	26	-13.278780268420956
2208	27	-5.283779935444677
2208	28	-2.0793428666542297
2208	29	-0.29696294595134987
2208	30	0.2892556465793277
2208	31	-0.10864414980161285
2208	32	1.0440091975872008
2208	33	0.1904384244245989
2208	34	1.0747680181874344
2208	35	1.08390432936541
2208	36	0.4728339838372264
2208	37	0.6282197628888042
2208	38	0.8353751871631019
2208	39	0.6951032168164701
2208	40	1.0107003257466118
2208	41	0.5744727971312358
2208	42	0.38966344224957083
2209	1	-1.97820400145374
2209	2	-3.436270809068837
2209	3	-4.254140291851002
2209	4	-5.7370018716666245
2209	5	-3.3469347172251354
2209	6	-3.827873843902907
2209	7	-3.228286128053991
2209	8	-4.488992267545733
2209	9	-3.7979877082005906
2209	10	-0.4716277933452595
2209	11	-3.3048479364735677
2209	12	-0.25100803296728813
2209	13	-0.3165546516640383
2209	14	1.127442647754556
2209	15	1.05178859606092
2209	16	-0.3351508570549129
2209	17	-0.06511551991694375
2209	18	-1.4536928094972765
2209	19	-0.7527817875665548
2209	20	-1.1073942663211227
2209	21	-1.1474427763502746
2209	22	-0.9222820119639508
2209	23	-0.6879410900602636
2209	24	-0.5393553862914473
2209	25	-3.1725245644976034
2209	26	-2.0000552869532022
2209	27	-1.8586502097219224
2209	28	-0.7105511691004835
2209	29	-1.6553765856844862
2209	30	-1.5921379783280116
2209	31	-1.421839109030671
2209	32	-1.6731887268012358
2209	33	-2.4226082768355894
2209	34	-2.574601885444885
2209	35	-1.3026783244522306
2209	36	-1.3433261347691499
2209	37	-1.8602902445240943
2209	38	-3.017478778737569
2209	39	-2.625133995933716
2209	40	-4.493747413319362
2209	41	-3.3491743489028636
2209	42	-2.9365040892552514
2215	1	-11.184776771406794
2215	2	-4.617021982777757
2215	3	-0.3104783200200174
2215	4	-0.7217436557041808
2215	5	1.4194972076574928
2215	6	4.028933667834185
2215	7	3.178990867251173
2215	8	3.2351861362101353
2215	9	-20.502213060313267
2215	10	-11.702848450622254
2215	11	-5.3130638989148835
2215	12	-21.462275638601092
2215	13	-0.2250053558893903
2215	14	-27.281008056470796
2215	15	-13.46933816450246
2215	16	-5.122709542500925
2215	17	-2.1120638767244557
2215	18	-1.3539275512813163
2215	19	0.4749177429498772
2215	20	0.7083334332093969
2215	21	0.4793177870300056
2215	22	1.9345254997731374
2215	23	0.7557208817707206
2215	24	1.1871704353047932
2215	25	10.645550552873285
2215	26	5.813324994736938
2215	27	3.312711292625501
2215	28	1.2671483614159484
2215	29	0.49251073825917757
2215	30	-0.39495487973645993
2215	31	0.2553909379176815
2215	32	0.8948863905696527
2215	33	0.6290349156526744
2215	34	0.3291539830997152
2215	35	0.3733780135759375
2215	36	0.26230766804775385
2215	37	0.781728534818626
2215	38	-0.37076516371408985
2215	39	0.05913830453576452
2215	40	0.19052488715011862
2215	41	-0.19307106251788753
2215	42	1.1045757229513242
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	4.0
25	34.0
26	295.0
27	1140.0
28	2516.0
29	4781.0
30	7367.0
31	9655.0
32	11455.0
33	12578.0
34	10896.0
35	7972.0
36	3432.0
37	1973.0
38	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.9901916833082012	2.6857399030036273	91.92511988697342	3.3989485267147574
2	80.65477803674996	2.69662312320451	15.637703940592987	1.0108948994525448
3	1.167477789611335	3.6251129056176445	1.1283821130539116	94.07902719171712
4	14.678404357145746	2.0275826738746514	81.8110735133532	1.482939455626407
5	2.0923076923076924	91.0468376068376	1.2882051282051281	5.572649572649572
6	0.5454797175688771	3.347639484978541	3.1773501315242973	92.92953066592828
7	2.2230610566617686	91.31941167747415	1.4397993987354571	5.017727867128625
8	91.79359314868015	3.657379803783403	1.4919507832402839	3.057076264296168
9	29.978212917980436	21.542146501211146	21.91293286601621	26.56670771479221
10	30.08483735004863	20.316383875499838	24.40694909758997	25.19182967686156
11	25.863073857706333	22.177643439012158	23.69166542418369	28.267617279097824
12	26.750390333310705	22.60403231280972	26.67707555495214	23.968501798927434
13	32.454992920234645	28.71283123187917	17.971815791248062	20.86036005663812
14	30.798515146588628	24.177640492060913	22.929875900937517	22.093968460412942
15	22.316426131684462	28.403828758893496	29.96989375059065	19.30985135883139
16	24.533211103172142	28.12192458578805	26.369359774593203	20.975504536446607
17	22.405587239952002	29.674122611872882	25.49178228100689	22.428507867168225
18	25.863812232902383	30.362511290561766	21.028081480782454	22.7455949957534
19	23.795785755692464	29.159982204726532	23.49784973778934	23.54638230179166
20	23.067797295657684	26.660555158607114	25.70068889278348	24.570958652951724
21	23.6690618385753	26.243983984253877	23.59626299257182	26.490691184599
22	23.7737438517351	26.517024919155414	25.59852169895921	24.110709530150277
23	22.176685495503996	28.020815077449885	26.72526524394354	23.07723418310258
24	20.340536824082935	29.741024845976515	27.1458807986303	22.772557531310245
25	20.11907413782629	30.14114740487232	27.409327474129725	22.330450983171666
26	21.21306082830026	30.883304572907676	26.578677739430546	21.324956859361517
27	22.236003073729055	28.576243309920862	27.726923439880284	21.460830176469795
28	22.19855478861087	28.408110440034513	28.315088438308887	21.07824633304573
29	21.717267040552198	29.769736842105267	27.16512079378775	21.34787532355479
30	21.77224746215134	29.192337247394747	28.833735524488723	20.201679765965192
31	22.378904512180327	28.897097483047308	27.925098076223087	20.79889992854928
32	22.94107337854052	29.792253663534517	27.097348234627983	20.16932472329698
33	20.885180042331182	31.325073809941085	27.511223155425533	20.278522992302193
34	20.912142577888023	30.402955093897035	28.25673726357227	20.428165064642677
35	21.394771964355527	31.489545276837834	26.66864391927417	20.447038839532468
36	20.469956994755787	31.151165455599443	28.328187982797903	20.050689566846867
37	20.743626730657752	29.546894589967245	28.626124000701026	21.08335467867398
38	21.909756393491246	29.92167383420737	27.83207732855198	20.33649244374941
39	22.27779500384216	30.66044730846489	27.30900413874921	19.752753548943744
40	20.788114914326545	31.25362309071545	27.79028539843887	20.167976596519136
41	20.87169877455276	31.907464577968913	27.51796378931475	19.70287285816358
42	20.778678026881646	31.81848821063133	27.579977621095487	19.822856141391537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	7.5
9	11.0
10	11.0
11	33.0
12	55.0
13	110.0
14	165.0
15	219.0
16	273.0
17	273.0
18	449.0
19	625.0
20	776.5
21	928.0
22	928.0
23	1096.0
24	1264.0
25	1401.0
26	1538.0
27	1673.0
28	1808.0
29	1808.0
30	2059.0
31	2310.0
32	2543.5
33	2777.0
34	2777.0
35	3096.5
36	3416.0
37	3703.0
38	3990.0
39	4287.0
40	4584.0
41	4584.0
42	4891.5
43	5199.0
44	5441.5
45	5684.0
46	6221.0
47	6758.0
48	6758.0
49	7217.0
50	7676.0
51	7326.0
52	6976.0
53	6976.0
54	6502.0
55	6028.0
56	5369.5
57	4711.0
58	3969.0
59	3227.0
60	3227.0
61	2540.0
62	1853.0
63	1447.5
64	1042.0
65	853.5
66	665.0
67	665.0
68	476.0
69	287.0
70	216.5
71	146.0
72	146.0
73	114.0
74	82.0
75	65.0
76	48.0
77	39.5
78	31.0
79	31.0
80	22.0
81	13.0
82	8.0
83	3.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.7630397562586786
2	0.5136363023578737
3	0.0
4	0.0
5	1.418229370289982
6	2.6248028364587404
7	0.0
8	0.5136363023578737
9	0.37747549779581274
10	0.20626339700985483
11	0.5392507111368753
12	0.7037221780336222
13	0.029658789112528144
14	0.4920662739123987
15	0.14290143845127196
16	0.0
17	0.010785014222737506
18	0.0
19	0.0
20	0.0
21	0.0
22	0.7805654043706269
23	0.0
24	0.0
25	33.4281515833749
26	0.0013481267778421883
27	0.0
28	0.0013481267778421883
29	0.0013481267778421883
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	74177.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.96248163177265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.46937103052653	70.64723566604204
2	6.499692685925015	10.264637286490421
3	1.8643720549067815	4.416463324211009
4	0.7273099774636346	2.2972080294430888
5	0.38072799289763026	1.5031613572940399
6	0.26804616540326437	1.2699354247273413
7	0.19975414874001232	1.1041158310527524
8	0.1109745270777846	0.7010259244779379
9	0.08365772041248379	0.594523909028405
>10	0.3721914908147238	5.2253393909163215
>50	0.018780304582394318	1.0407538724941694
>100	0.005121901249743905	0.9355999838224787
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCTACACCGGTAGGTTGAGGGTAGAGGATGGACATCAGTAA	340	0.458363104466344	No Hit
TGCTACACCGGTAGGTTGAGGGTANAGGATGGACATCAGTAA	213	0.2871510036803861	No Hit
CGCTATAGGTACGTACGATCGTCGGACTGTAGAACTCTGAAC	141	0.19008587567574856	Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGCTATAGGTACGTACGATCGTCGNACTGTAGAACTCTGAAC	93	0.1253757903393235	Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGCTACAGGCCCGACGATCTCCCTGCTACAGTTTTTTTTTTT	91	0.12267953678363913	No Hit
CGATACAGGTCAGTGAAACCCCGTCTCTACTAAAAATACAAA	82	0.11054639578305944	No Hit
TTCGACAGTAACTGAGATCATGCCATTGCACTCTAGCCTGGG	80	0.10785014222737506	No Hit
TGCTACAGGTTCGGCGGAGTCCTTGGATCTTGGGGATGGCGC	78	0.10515388867169068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.006740633889210941	0.0	0.0
13	0.0	0.0	0.056621324669371906	0.0	0.0
14	0.0	0.0	0.08223573344837348	0.0	0.0
15	0.0	0.0	0.10919826900521724	0.0	0.0
16	0.0	0.0	0.13750893133990322	0.0	0.0
17	0.0	0.0	0.3923048923520768	0.0	0.0
18	0.0	0.0	0.5041994149129784	0.0	0.0
19	0.0	0.0	0.5486875985817706	0.0	0.0
20	0.0	0.0	0.6471008533642504	0.0	0.0
21	0.0	0.0	0.7023740512557801	0.0	0.0
22	0.0	0.0	0.7967429257047333	0.0	0.0
23	0.0	0.0	0.8992005608207396	0.0	0.0
24	0.0	0.0	1.0191838440486944	0.0	0.0
25	0.0	0.0	1.117597098831174	0.0	0.0
26	0.0	0.0	1.245669142726182	0.0	0.0
27	0.0	0.0	1.3643042991762946	0.0	0.0
28	0.0	0.0	1.4883319627377758	0.0	0.0
29	0.0	0.0	1.6972916133033151	0.0	0.0
30	0.0	0.0	1.8577186998665354	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGCG	15	0.007965572	50.650253	20
ACACAAT	15	0.008360783	50.034447	23
CCAGTCA	15	0.008360783	50.034447	25
TGAGCGT	25	0.0011673905	40.520206	22
CATGCTC	25	0.0012399222	40.027557	24
ATGCTCG	25	0.0012399222	40.027557	25
GAGGATG	65	1.10958354E-10	38.488037	25
TAGAGGA	60	2.1882443E-9	37.525833	23
CGCGACA	20	0.0025238334	34.655437	1
TGCGGCA	25	1.7959195E-4	34.655437	1
CGCTATA	55	2.5465852E-11	34.655437	1
TGCTCTA	20	0.0025238334	34.655437	1
AGAGGAT	65	4.78758E-9	34.639233	24
CAGGTCA	105	0.0	34.631138	6
CAGCGTA	25	1.8033518E-4	34.631138	6
CAGCGGC	25	1.8033518E-4	34.631138	6
CAGCCGG	50	3.5288394E-10	34.631138	6
CAGGCCG	50	3.5288394E-10	34.631138	6
CAGGCAT	25	1.8033518E-4	34.631138	6
CAGGCAC	25	1.8033518E-4	34.631138	6
>>END_MODULE