FastQCFastQC Report
Thu 26 May 2016
SRR2099010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249075
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGCATCATGACAAGATCGTCACTGAATGCACGGTCATGGA5000.2007427481682224No Hit
ACGTGCATCAGTTGAACAAGAAAGGGGTAAAGGCTATCCTGCT3440.13811101073973703No Hit
CCGTGCATCATGACAAGATCGTCACNGAATGCACGGTCATGGA2960.11883970691558766No Hit
ACGCGCTTAATGGGTTCTACAGTCCGACGATCTCACGACGTGC2780.11161296798153167Illumina Small RNA Adapter 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCA259.718905E-442.11712326
CGCATAC251.5408112E-435.6130944
GCGTCTC200.002218329635.6130945
GCATAGC2750.035.6130945
GCATAAC2050.035.613095
GCCTTGT301.0764941E-535.613095
CGCGCTT1050.035.538532
CGCGCCT251.5599282E-435.538532
ACGCGCT1050.035.538531
TCGTGCT301.0920778E-535.5385251
GTGCATC47750.035.3520553
GCATACT3950.035.1622925
GTGCATA40100.035.1246383
AACTACC300.002380548335.097625
GCATTCG2950.035.0094835
CGTGCGT6650.035.0041122
ATAGCAC454.483809E-934.9962927
ATTCGTT454.483809E-934.9962927
TGATACG200.002416940234.996298
GTCGCAA406.222217E-834.9962910
Produced by FastQC (version 0.11.5)