FastQCFastQC Report
Thu 26 May 2016
SRR2099009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473279
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACCGAAGCTGGCTGATCGTCGGACTGTAGAACTCTGAACC7980.16861090392770436Illumina DpnII expression Sequencing Primer (100% over 27bp)
TGCACGTAAAACAGCCGACGATCAAAAATACGTGCATGTTTGA7060.1491720528483199No Hit
TGCACGTAAAAAAACTCACTATAGTGAGTCGTATTACCGGCCG6550.13839616801083504No Hit
AACACGTACATGGGGCCGGTAGAACTCTGAACCCCTATAGTGA6210.13121224478584512No Hit
TGCACGTAAAACAGCCGACGATCAANAATACGTGCATGTTTGA5250.11092822626822656No Hit
TGCACCGAAGCTGGCTGATCGTCGGNCTGTAGAACTCTGAACC5130.10839272395352426Illumina DpnII expression Sequencing Primer (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACC1150.041.450525
GAACTTA2200.040.92713526
ATAATGA2450.036.0608621
CGTAGTC5200.035.4942785
CGCGTAG1250.035.4942783
CGCGTAC950.035.4942783
CGTCCCG453.921741E-935.4942785
GCGTACG200.002257151535.4942784
GGTAAGC251.5736304E-435.4942745
CGTACGG4950.035.4942745
CGTCGCG502.8012437E-1035.4942745
CACGTAC91100.035.357913
ACGTAGT21300.035.3276374
ATCTACC605.761285E-835.30968524
CACGTAG80900.035.296843
GCCCGTA13100.035.257912
CGTATGG5650.035.180175
ACGTATA23750.035.1206554
CGTACCG4500.035.0998955
ACGTACC22400.035.0981374
Produced by FastQC (version 0.11.5)