##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473279
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.444334103140008	30.0	27.0	31.0	18.0	35.0
2	28.580239140126647	30.0	27.0	32.0	19.0	35.0
3	29.002700309965157	30.0	28.0	32.0	21.0	36.0
4	25.2432751083399	28.0	21.0	31.0	10.0	35.0
5	28.307353590588214	30.0	27.0	32.0	17.0	37.0
6	28.329182152599206	30.0	26.0	32.0	17.0	37.0
7	27.28125270717695	30.0	25.0	31.0	13.0	37.0
8	24.229815816886024	27.0	17.0	30.0	8.0	34.0
9	26.111900168822196	27.0	26.0	28.0	22.0	31.0
10	26.239725405099318	27.0	26.0	28.0	22.0	31.0
11	25.69032853771243	27.0	26.0	27.0	18.0	31.0
12	28.133308260032667	30.0	26.0	31.0	18.0	35.0
13	27.660153947248876	30.0	26.0	31.0	17.0	35.0
14	31.35291868010201	31.0	31.0	34.0	30.0	34.0
15	31.319887423697228	31.0	31.0	34.0	30.0	34.0
16	31.53530792619153	31.0	31.0	34.0	30.0	34.0
17	35.404275279486306	37.0	35.0	37.0	33.0	37.0
18	35.443769953874984	35.0	35.0	37.0	33.0	37.0
19	35.097703468778455	35.0	35.0	37.0	32.0	37.0
20	35.03258754349971	35.0	35.0	37.0	32.0	37.0
21	34.999575303362285	35.0	35.0	37.0	32.0	37.0
22	36.54582814787895	37.0	35.0	39.0	32.0	39.0
23	36.473236716609016	37.0	35.0	39.0	33.0	39.0
24	36.7742895839452	38.0	35.0	39.0	33.0	39.0
25	36.99063343186577	38.0	37.0	39.0	33.0	39.0
26	22.797045717219653	32.0	2.0	37.0	2.0	39.0
27	29.843489780869213	35.0	17.0	39.0	17.0	40.0
28	34.86942585663002	36.0	32.0	39.0	27.0	40.0
29	36.92828542994724	38.0	36.0	40.0	33.0	41.0
30	37.70424844542014	38.0	37.0	40.0	34.0	41.0
31	38.08178262716072	39.0	37.0	40.0	34.0	41.0
32	38.37537900477308	39.0	38.0	40.0	35.0	41.0
33	38.43944269659123	40.0	38.0	40.0	35.0	41.0
34	38.416088607354226	40.0	38.0	40.0	35.0	41.0
35	38.41367776723666	40.0	38.0	40.0	35.0	41.0
36	38.39648917446158	40.0	38.0	40.0	34.0	41.0
37	38.41327208686631	40.0	38.0	40.0	35.0	41.0
38	38.37710948510287	40.0	38.0	40.0	34.0	41.0
39	38.259100868620834	40.0	38.0	40.0	34.0	41.0
40	38.17451228556517	40.0	38.0	40.0	34.0	41.0
41	38.115557630911155	40.0	38.0	40.0	34.0	41.0
42	38.02783558957824	40.0	37.0	40.0	34.0	41.0
43	37.976757895448564	40.0	37.0	40.0	34.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	6.382729654838972
1101	2	5.921643422238368
1101	3	5.579681976833836
1101	4	7.664915627466314
1101	5	8.537712954527887
1101	6	7.585330750922168
1101	7	6.814439747326592
1101	8	5.703900256915478
1101	9	6.088897554321303
1101	10	5.780232007273554
1101	11	3.7674396433286077
1101	12	8.054849250256702
1101	13	6.911477402440788
1101	14	0.13763960210105353
1101	15	1.1582754941592874
1101	16	1.6350108362103484
1101	17	0.9018219251117543
1101	18	0.32297529302698536
1101	19	0.09258113084886332
1101	20	-0.030498487274236652
1101	21	0.01912019930300346
1101	22	0.12860579002978056
1101	23	1.003192404710802
1101	24	0.6615259517245704
1101	25	0.4517983983203564
1101	26	12.62167199130172
1101	27	7.4576025396480325
1101	28	3.0954942955412648
1101	29	1.4114567822838282
1101	30	0.8426780043948696
1101	31	0.395450529054699
1101	32	0.2016576146457254
1101	33	0.1547668537121183
1101	34	0.15844109902358383
1101	35	0.292662245295233
1101	36	0.155992577845538
1101	37	0.11015527567391814
1101	38	-0.002613309412303977
1101	39	0.08530998557314717
1101	40	0.015146455633008316
1101	41	-0.009077353219524298
1101	42	-0.03009178999475637
1101	43	0.06294785663242664
1102	1	3.6290015638868134
1102	2	2.4202990125839428
1102	3	1.8784152092632702
1102	4	4.128111662174174
1102	5	5.264134601880318
1102	6	3.3403785262222847
1102	7	3.435569948017516
1102	8	1.7102128356262973
1102	9	4.2837528419551845
1102	10	3.7837194308735143
1102	11	3.980938736348911
1102	12	5.6215271627013905
1102	13	4.297084038990334
1102	14	0.028298519964508273
1102	15	1.538058512264218
1102	16	1.8484516976677554
1102	17	1.0537182636866973
1102	18	0.5219580892973923
1102	19	0.24145774694804345
1102	20	0.21236733749960024
1102	21	0.17704858834530057
1102	22	0.2510339537713904
1102	23	1.0805749883897917
1102	24	0.6887845622547744
1102	25	0.3550374134674996
1102	26	12.644999047026094
1102	27	7.560654844069699
1102	28	3.165330577040038
1102	29	1.4531776173983886
1102	30	0.6993819130729904
1102	31	0.49686934570615193
1102	32	0.2551980705618888
1102	33	0.20914196202976143
1102	34	0.21853191253237725
1102	35	0.21888262403147252
1102	36	0.11829734613244369
1102	37	-0.025042006853141174
1102	38	0.020875962808702297
1102	39	0.02342531641854606
1102	40	0.0015835851866796702
1102	41	-0.09698090141654347
1102	42	-0.05401340846248104
1102	43	-0.06569675752569992
1103	1	0.5642151390769854
1103	2	0.10231730883478107
1103	3	0.013902514633688412
1103	4	1.1200264889877438
1103	5	2.026955820964364
1103	6	-2.335907135054459
1103	7	-1.0164325067056836
1103	8	-2.1217076998458815
1103	9	2.34402942646474
1103	10	1.6914252636954465
1103	11	-0.9459139374440646
1103	12	2.1065453590507026
1103	13	1.505319349898155
1103	14	-0.3797561118983701
1103	15	1.4786256674969174
1103	16	1.6940484665669828
1103	17	0.9917390717473253
1103	18	0.4944275122947204
1103	19	0.21635420615254475
1103	20	0.2648110715331953
1103	21	0.22571005061820415
1103	22	0.16558503913010725
1103	23	1.102435440230117
1103	24	0.7677218954549119
1103	25	0.3868123781451729
1103	26	12.50815365177284
1103	27	7.449145902883156
1103	28	3.094977068355739
1103	29	1.5494738396935546
1103	30	0.7588373070417944
1103	31	0.41250821596261744
1103	32	0.3364377057786072
1103	33	0.20183231011521485
1103	34	0.16803197675478998
1103	35	0.17415956533218946
1103	36	0.1831425701259377
1103	37	0.15753145644731603
1103	38	0.1492011850895949
1103	39	0.0981719233345828
1103	40	0.11138563744945174
1103	41	0.2043029759043833
1103	42	0.16780165954191517
1103	43	0.17195584205470738
1104	1	1.3941727442649459
1104	2	-0.678660071130551
1104	3	-0.5414462013079131
1104	4	1.1904774036650068
1104	5	1.0958800559725717
1104	6	-0.5415983672308613
1104	7	-0.04022401237482143
1104	8	-0.05246921878750399
1104	9	2.167110395203686
1104	10	1.1237485944763392
1104	11	0.872750244755256
1104	12	2.7018768766737473
1104	13	1.2337506641134084
1104	14	0.38175714877665357
1104	15	1.972967594109253
1104	16	1.8707202271491674
1104	17	1.0168916126404213
1104	18	0.3101241473504075
1104	19	0.11553653852799073
1104	20	0.177428842790178
1104	21	0.053691284358357905
1104	22	-0.07651268929851085
1104	23	0.7822091471943295
1104	24	0.5468099121556946
1104	25	0.2452641168586993
1104	26	12.39560961322373
1104	27	7.395527035203468
1104	28	3.085955725039092
1104	29	1.3661571654871736
1104	30	0.7097329979839415
1104	31	0.35706725938687356
1104	32	0.2147147523919699
1104	33	0.12452837976721298
1104	34	0.0266374000256846
1104	35	0.079369681124831
1104	36	-0.017603547470251613
1104	37	0.01774398963226531
1104	38	-0.22500486523821195
1104	39	0.016142641343449782
1104	40	-0.06786616991383454
1104	41	-0.10080482304870486
1104	42	-0.11810752962852433
1104	43	-0.14729452414210442
1105	1	1.0951741033764861
1105	2	-1.9028945386711307
1105	3	-1.069318542922371
1105	4	-1.239177553366826
1105	5	-2.121556059886565
1105	6	-1.8443418602282406
1105	7	-6.036999770146249
1105	8	-3.242502690556911
1105	9	1.4868598567434361
1105	10	0.6444869974799197
1105	11	1.1532832226127283
1105	12	2.77217411169822
1105	13	0.36893538593636777
1105	14	0.17314159852895372
1105	15	1.8326292380443796
1105	16	1.6584905205347198
1105	17	0.8313598486080167
1105	18	0.19155237551971993
1105	19	-0.12088585024248033
1105	20	-0.08262836531283568
1105	21	0.12088938495496393
1105	22	-0.1186439053401287
1105	23	0.8297460757264261
1105	24	0.46535511022027976
1105	25	0.15463081061653838
1105	26	12.396202555902004
1105	27	7.121214367526417
1105	28	2.8918753418801515
1105	29	1.2353356946292209
1105	30	0.5005869037377195
1105	31	0.18091640731893222
1105	32	0.028495050676283995
1105	33	-0.1594666382725265
1105	34	-0.1651494436007468
1105	35	-0.07357507089784576
1105	36	-0.1534010802078285
1105	37	-0.17445729298656687
1105	38	-0.26012631914607454
1105	39	-0.23272440352337043
1105	40	-0.327052280029811
1105	41	-0.40005057512664166
1105	42	-0.48272691941289736
1105	43	-0.3693816699519843
1106	1	-4.281480551198722
1106	2	-1.768955624596952
1106	3	-6.803376696058585
1106	4	-1.3159591947085794
1106	5	0.8931881997257847
1106	6	-1.5515844521944366
1106	7	-4.533220402094383
1106	8	-2.3667579188401895
1106	9	-0.26331919081802013
1106	10	0.5935316677206082
1106	11	0.9210146002490731
1106	12	2.9147403781928
1106	13	-4.054993493915632
1106	14	0.2924581376104527
1106	15	1.9043559746167595
1106	16	1.7869351783034944
1106	17	0.8321903647374143
1106	18	0.21948568479107422
1106	19	-0.10719225494855067
1106	20	0.0360163910974407
1106	21	0.09994507205352932
1106	22	0.024669731186705235
1106	23	0.9895944474801865
1106	24	0.34362733432168824
1106	25	0.2984952900369322
1106	26	12.359324427293888
1106	27	7.2829117950995546
1106	28	2.8988700073021505
1106	29	1.387470376189114
1106	30	0.6432221436992478
1106	31	0.34092351253087116
1106	32	-0.01226921313116236
1106	33	0.024892824981620265
1106	34	0.030220263630511113
1106	35	-0.14679654753474125
1106	36	-0.12764279725032424
1106	37	-0.2440808112380779
1106	38	-0.07690350083352371
1106	39	0.020100201674395635
1106	40	-0.14293942054104747
1106	41	-0.20405065827274171
1106	42	0.013703467462114816
1106	43	-0.08509288088040279
1107	1	2.1788092561945085
1107	2	1.2549706823775644
1107	3	-4.364781319817407
1107	4	-21.09822980124046
1107	5	-23.743670276168462
1107	6	-10.02224107978147
1107	7	-6.385703504140082
1107	8	-2.579137539608226
1107	9	1.2330656937375473
1107	10	1.5914680237440564
1107	11	2.0181479295946865
1107	12	3.7805773503186515
1107	13	2.750130474262381
1107	14	0.47784589332285776
1107	15	1.9295571051347622
1107	16	1.8146149246846406
1107	17	0.749386650178252
1107	18	0.2117470198790059
1107	19	-0.058584466935634794
1107	20	-0.22071662122460367
1107	21	0.1274378941614316
1107	22	0.3397605016137959
1107	23	1.1583048195342585
1107	24	0.7465612977144431
1107	25	0.49765936468701
1107	26	12.596636007626469
1107	27	7.604118727746997
1107	28	3.141034141767996
1107	29	1.3950819325953816
1107	30	0.6709766597896873
1107	31	0.1890363250682583
1107	32	-0.020650897543177393
1107	33	-0.23107379782015158
1107	34	-0.39189960716743144
1107	35	-0.14021681311674428
1107	36	-0.3192401773197133
1107	37	-0.33931498996759046
1107	38	-0.3731859508873612
1107	39	-0.2386478543131645
1107	40	0.01039456079643486
1107	41	0.11923861077858078
1107	42	-0.39022119457879967
1107	43	0.05097303609530712
1113	1	-7.35576034087509
1113	2	-0.7615128341059503
1113	3	0.4263130025269142
1113	4	2.1398654368547767
1113	5	-23.743670276168462
1113	6	-10.067914156704546
1113	7	-0.5584370206235967
1113	8	1.8438249146042303
1113	9	-21.197222767800913
1113	10	-21.812378130102097
1113	11	-11.696366722419963
1113	12	-22.284326495835195
1113	13	-10.772648829766924
1113	14	-0.07927407004710574
1113	15	1.8010085703362293
1113	16	1.6071973422670602
1113	17	0.4676787747203832
1113	18	0.3758953715273563
1113	19	0.04329282244165711
1113	20	0.6756890564310751
1113	21	0.12308807731161409
1113	22	-0.868687300584007
1113	23	0.8936528048822439
1113	24	0.22320964936279353
1113	25	-0.023173968646325704
1113	26	12.30611402960449
1113	27	7.493198398076668
1113	28	3.0522063029401565
1113	29	1.677591090104542
1113	30	1.172808161621191
1113	31	1.3680656290975577
1113	32	1.096336281944005
1113	33	-0.7553411970875459
1113	34	0.9048036895358678
1113	35	0.15465498175505132
1113	36	-0.15097094655047982
1113	37	0.037745449592847535
1113	38	0.11353566083425193
1113	39	0.6482569075915947
1113	40	0.6458112274630992
1113	41	0.803762420302391
1113	42	1.1641515160439084
1113	43	1.2519803621026355
1114	1	-2.0024690243484784
1114	2	-1.299327960156372
1114	3	-7.417524532487089
1114	4	-2.186465095358109
1114	5	0.10191795912565382
1114	6	-0.28850239199866223
1114	7	1.844924323914217
1114	8	2.618684858581819
1114	9	-4.035458061918561
1114	10	0.6949748110743741
1114	11	1.29558005629152
1114	12	-22.284326495835195
1114	13	-8.957872919402778
1114	14	-0.563867907582118
1114	15	-27.81803904871139
1114	16	-14.140701817396806
1114	17	-6.491704978781019
1114	18	-2.5894407629264293
1114	19	-1.346763199967306
1114	20	-1.3481204673784504
1114	21	-0.7778222868340379
1114	22	-0.5325528468024956
1114	23	0.07572563401389232
1114	24	-0.646538249796869
1114	25	-0.22660534119534503
1114	26	11.885945962377601
1114	27	6.678072347656499
1114	28	2.4464640060213867
1114	29	0.37471994164515365
1114	30	-0.23965682437320623
1114	31	-0.3878867518548219
1114	32	-0.5458205808010987
1114	33	-0.7276801326617743
1114	34	-0.8998181592036332
1114	35	-0.3257371751076974
1114	36	-0.4009709465504798
1114	37	-0.6376747184743792
1114	38	-0.5688873083534247
1114	39	-0.45188314843080946
1114	40	-0.41791426273297816
1114	41	-0.19693785980965117
1114	42	-0.14432187331183144
1114	43	-0.4829636154884014
1115	1	-3.5985394197197422
1115	2	-0.22989690904740456
1115	3	-0.23020534251601887
1115	4	-0.5900330799289861
1115	5	2.69895267465121
1115	6	1.522249777721683
1115	7	2.5141625109923282
1115	8	1.7868381854863529
1115	9	-21.197222767800913
1115	10	-21.812378130102097
1115	11	-8.981300368009347
1115	12	-22.284326495835195
1115	13	-8.5392374324211
1115	14	-0.18778304740854068
1115	15	0.9524527545672967
1115	16	-27.964231229161513
1115	17	-16.02256322371834
1115	18	-5.38054490950309
1115	19	-1.5172067872382797
1115	20	-1.389104073935826
1115	21	-1.189948612774252
1115	22	-0.493980040630845
1115	23	-0.1711403254846573
1115	24	-0.23814866445454186
1115	25	-0.39475866809987536
1115	26	11.919697167777795
1115	27	6.3222382107230395
1115	28	2.043619261566228
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2206	7	0.6070273952312384
2206	8	-0.2075124344947028
2206	9	2.1364576070038765
2206	10	1.5796052119072748
2206	11	1.244239608608023
2206	12	3.107136283966991
2206	13	1.8842015209928498
2206	14	0.27371668377654856
2206	15	1.9992436165671101
2206	16	1.8915730394485877
2206	17	0.9999660259040368
2206	18	0.40854205852073733
2206	19	0.3066725106001087
2206	20	0.23100498811348302
2206	21	0.2368185252430024
2206	22	0.30294949572743235
2206	23	1.1256996059035345
2206	24	0.7404997512442506
2206	25	0.41635405495249955
2206	26	-7.396719316123182
2206	27	-4.281323800183166
2206	28	-1.6986427092310876
2206	29	-0.641092135398047
2206	30	-0.16873022364442392
2206	31	0.062074205979641306
2206	32	0.17684990332175943
2206	33	0.23256192876456083
2206	34	0.15757382413838172
2206	35	0.1862357556575276
2206	36	0.23997127104452431
2206	37	0.09039659854800419
2206	38	0.1271694329271611
2206	39	0.1414276272068804
2206	40	0.15717683565328144
2206	41	0.1629007659061159
2206	42	-0.08048793265592735
2206	43	0.03493071038354145
2207	1	0.21906959109769986
2207	2	-4.279653423675111
2207	3	-1.1451155689016552
2207	4	-0.9315631345737927
2207	5	1.3368285900446892
2207	6	-2.540703272350804
2207	7	-0.24891321109978648
2207	8	0.5490403341053636
2207	9	1.301643445351015
2207	10	1.1263973801019844
2207	11	1.4362863388045248
2207	12	1.2802993545049404
2207	13	-1.2170932742113685
2207	14	0.274467426551535
2207	15	1.879239862853236
2207	16	1.718308453378171
2207	17	0.9030529243802405
2207	18	0.31807224227565456
2207	19	0.08184157527612257
2207	20	0.07591581380069101
2207	21	0.04712435849075547
2207	22	0.1777979601869646
2207	23	0.9922922606645557
2207	24	0.6438445699977109
2207	25	0.48929768668247675
2207	26	6.202939426429886
2207	27	4.142858262022248
2207	28	1.734745985479833
2207	29	0.6061625186759727
2207	30	0.36215056524930844
2207	31	0.03813365630844601
2207	32	0.17003242706871902
2207	33	0.007697351665285623
2207	34	-0.030570460123996668
2207	35	0.06735339445346256
2207	36	0.01682950696425678
2207	37	0.0048656309987435975
2207	38	0.11807080822654115
2207	39	0.056420172897716725
2207	40	-0.0832137158475561
2207	41	0.09854700080125411
2207	42	-0.0025151506227558684
2207	43	0.1771504301298492
2208	1	-24.450998436113185
2208	2	-10.47178248413464
2208	3	-2.0820059877197856
2208	4	3.9579950983579337
2208	5	3.9792212900965964
2208	6	2.7031701806448503
2208	7	-24.272722734909312
2208	8	-7.954224425613786
2208	9	3.292737071556516
2208	10	2.978786528532442
2208	11	-21.505890531943773
2208	12	-7.179908825152463
2208	13	1.7609139929525242
2208	14	0.890892309757831
2208	15	2.495213963336802
2208	16	2.320909333087485
2208	17	1.6007826186675942
2208	18	0.8951151076145578
2208	19	0.7248762992058531
2208	20	0.6217590506938393
2208	21	0.491992265492911
2208	22	0.7348697849007877
2208	23	1.879883441715286
2208	24	1.4934334015142525
2208	25	0.900520810470141
2208	26	-22.408171684681225
2208	27	-13.366813076392635
2208	28	-4.201953192183197
2208	29	-1.322982633360752
2208	30	-0.17085219408735952
2208	31	0.18390039673955272
2208	32	0.5822800570444073
2208	33	0.8132187570145746
2208	34	0.8784124101325403
2208	35	0.7691128130803477
2208	36	0.7968202181081523
2208	37	0.6837580715090823
2208	38	0.612101352171031
2208	39	0.8100469248033022
2208	40	0.9791445607964349
2208	41	1.0504635103769715
2208	42	0.9363833003238824
2208	43	1.277224194575382
2209	1	-2.296452981567729
2209	2	-5.2303440029371195
2209	3	-3.595332019118107
2209	4	-2.498229801240459
2209	5	-3.589124821623006
2209	6	-8.040641429431819
2209	7	-5.945450007636584
2209	8	-5.9998980291187145
2209	9	-1.81540458598273
2209	10	0.033076415352446986
2209	11	-2.0786178046710475
2209	12	-0.02069013219883331
2209	13	-1.1735146306327238
2209	14	-0.43338662415965956
2209	15	1.6092336785613348
2209	16	1.2903142253839412
2209	17	0.31096881801042286
2209	18	-0.3072215115895318
2209	19	-0.19783271868388397
2209	20	-0.575393194651177
2209	21	-0.7474746932511565
2209	22	-0.5518041837008951
2209	23	0.7062069174363543
2209	24	-0.6599072337540903
2209	25	0.037432091959736624
2209	26	-2.335444411953951
2209	27	-0.9743340694558
2209	28	-1.1495252987914455
2209	29	-1.2232747107612596
2209	30	-0.6367156479026193
2209	31	-0.7037958427639097
2209	32	-0.5824515968438746
2209	33	-1.0791507208970685
2209	34	-0.8653261805940033
2209	35	-1.915041987941919
2209	36	-1.0600618556413863
2209	37	-1.611605199757804
2209	38	-1.0674167201181248
2209	39	-1.0500114906768019
2209	40	-1.5208554392035651
2209	41	-2.6693977528577832
2209	42	-1.531086579194188
2209	43	-1.406894096771822
2215	1	-4.325998436113185
2215	2	-0.9914235483916656
2215	3	-0.8780024736635603
2215	4	2.3861451987595395
2215	5	3.100079723831538
2215	6	1.9008358432954537
2215	7	3.071027265090688
2215	8	0.7694201526994675
2215	9	-21.197222767800913
2215	10	-11.015503130102097
2215	11	-4.037140531943773
2215	12	-22.284326495835195
2215	13	-12.188571448814542
2215	14	-0.30100026052329554
2215	15	-27.81803904871139
2215	16	-13.823606229161513
2215	17	-4.611190272898668
2215	18	-2.3651760570440743
2215	19	-1.095844082320248
2215	20	-0.31687046737845037
2215	21	-0.3136678750693349
2215	22	0.1558662708445624
2215	23	1.0104682810727184
2215	24	0.5290132207913629
2215	25	0.13828436468701
2215	26	9.513703315318775
2215	27	5.785609112362383
2215	28	2.6578610648449157
2215	29	1.1522934710569217
2215	30	0.7539093520973807
2215	31	0.1589882481451781
2215	32	0.5130029486106693
2215	33	0.7364742791029286
2215	34	0.35569654667872186
2215	35	0.3213216484217156
2215	36	0.005279053449520177
2215	37	0.5957811638785628
2215	38	0.09195827988187233
2215	39	-0.3167728543131645
2215	40	0.49476956079643486
2215	41	0.3223636107785808
2215	42	0.41266342080581353
2215	43	-0.24950773313545938
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	7.0
25	133.0
26	907.0
27	3684.0
28	9822.0
29	20380.0
30	36586.0
31	55083.0
32	75998.0
33	90842.0
34	77727.0
35	59325.0
36	24816.0
37	17036.0
38	932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.7198553916935188	1.296334451314708	97.63163540354596	0.3521747534458112
2	96.30999116798161	1.3919937119409733	1.4503103883975168	0.8477047316799007
3	0.6997986388578408	1.35966311625912	0.34356056364216453	97.59697768124087
4	1.9624249367634472	92.35454520099462	0.7575513776879558	4.925478484553976
5	0.40313872291411706	1.962247192972123	0.8512160697028598	96.7833980144109
6	95.26782752376546	1.5126639916665081	0.46865326631042253	2.7508552182576156
7	2.9223158471593553	2.005560853967047	92.3804830593618	2.6916402395118
8	2.32484433072247	90.36572507970986	1.4741833041398413	5.835247285427834
9	23.436936688566448	24.884951923688664	25.97435647301831	25.703754914726577
10	24.87108407965151	28.284417638874476	23.83509774315078	23.009400538323234
11	21.86737565139034	24.414067481063988	30.368391522786975	23.3501653447587
12	23.330752772330715	24.232956599784764	28.293299190526223	24.1429914373583
13	20.906054556697413	26.399168105578703	29.33091125389683	23.363866083827055
14	29.945067138989227	29.81655933023759	18.925099815481385	21.313273715291796
15	28.933335879628157	25.995972610355356	21.68170041568263	23.388991094333857
16	22.241357059591387	26.559389340109043	30.88292229869105	20.316331301608525
17	23.733358124911522	28.96832523733358	26.294426754620424	21.003889883134473
18	21.64490729196164	29.65516075451283	25.456777809241643	23.243154144283885
19	24.158317099041156	28.008696791315042	22.94338633952983	24.88959977011397
20	23.423181675079604	27.246930457510267	26.182864652773524	23.147023214636608
21	21.483099820613212	29.727496888727366	25.37425070624304	23.41515258441638
22	21.426262310391966	26.156875754047825	28.181896936056745	24.234964999503465
23	22.25030691032261	27.364273298057263	27.77809692196074	22.607322869659388
24	22.038797411252137	30.2821380200685	27.026130464271603	20.652934104407763
25	20.463194014524202	28.635117974809788	28.791051367163977	22.110636643502033
26	19.256276257140136	29.71413180222112	28.890655332231862	22.138936608406883
27	21.327180449380275	29.52496862282737	27.500982517526847	21.646868410265512
28	21.2881673945702	29.153328811668434	28.94309252298337	20.615411270778
29	22.15885680236821	28.376462434683024	28.375194656738557	21.08948610621021
30	21.299698486516412	29.090029348439295	28.462915109269588	21.147357055774712
31	21.833844307480366	29.54789880810262	28.572998168099577	20.045258716317434
32	21.067488732861587	29.717777463187677	28.710549168672177	20.50418463527856
33	21.405344416295673	29.602200816009162	28.183587270933213	20.808867496761952
34	21.747848520640044	29.287587237126516	28.481931376629856	20.482632865603588
35	20.539047792105713	29.570507037075384	28.30719300877495	21.583252162043955
36	20.39896128921841	29.390908956450634	29.148134609817888	21.06199514451307
37	21.313009873668598	29.525713162848977	28.877681029582973	20.283595933899452
38	21.002410840117562	29.385626659961673	29.449014217829227	20.162948282091538
39	21.387384608233198	30.622106622098173	28.77964160674782	19.210867162920813
40	21.13911667325193	29.762782629273644	29.566492491743773	19.53160820573066
41	20.91831668001327	30.0156989851652	29.5561391906254	19.50984514419613
42	22.103875303996162	29.145176523784066	29.47901766188654	19.27193051033323
43	21.19637676719229	30.733034848366398	29.14242972960981	18.928158654831506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	8.5
9	16.0
10	78.0
11	140.0
12	140.0
13	320.5
14	501.0
15	889.5
16	1278.0
17	1965.5
18	2653.0
19	2653.0
20	3431.5
21	4210.0
22	5238.0
23	6266.0
24	7451.5
25	8637.0
26	8637.0
27	9871.0
28	11105.0
29	12899.5
30	14694.0
31	16574.0
32	18454.0
33	18454.0
34	20788.5
35	23123.0
36	25491.5
37	27860.0
38	30762.5
39	33665.0
40	33665.0
41	36880.0
42	40095.0
43	42091.0
44	44087.0
45	43717.0
46	43347.0
47	43347.0
48	42146.5
49	40946.0
50	39561.5
51	38177.0
52	35799.0
53	33421.0
54	33421.0
55	30840.5
56	28260.0
57	24402.5
58	20545.0
59	17566.0
60	14587.0
61	14587.0
62	11487.0
63	8387.0
64	6571.0
65	4755.0
66	3459.5
67	2164.0
68	2164.0
69	1601.5
70	1039.0
71	723.0
72	407.0
73	303.0
74	199.0
75	199.0
76	181.5
77	164.0
78	117.5
79	71.0
80	40.5
81	10.0
82	10.0
83	11.5
84	13.0
85	7.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.5261167303007317
2	2.112918595585268E-4
3	0.0
4	1.5153852167537543
5	3.1431777027926446
6	0.0016903348764682143
7	0.5256941465816146
8	0.0
9	0.36701396005316106
10	0.2265048734467407
11	0.5804187382072731
12	0.6543708890527575
13	1.2491574737100104
14	0.03296153009113018
15	0.42385147027440473
16	0.12888803433070134
17	0.0
18	0.015424305747772454
19	2.112918595585268E-4
20	0.0
21	0.0
22	0.0
23	0.6913469644754996
24	0.0
25	0.0
26	35.67409498414255
27	0.001056459297792634
28	4.225837191170536E-4
29	0.002112918595585268
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	473279.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.57998704146755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36644280148059	66.78734601836484
2	7.759940279474408	11.729923715304757
3	1.7688802370584968	4.010758361843677
4	0.6318303945478271	1.9101493212932037
5	0.3646495806532141	1.3780105290223248
6	0.21840181287680221	0.9904083712237037
7	0.1598455081342428	0.8456785003396006
8	0.10678232855359383	0.645648560667068
9	0.07626033966992006	0.5187379935627424
>10	0.5026789827463004	6.808769767825649
>50	0.028262567934624543	1.4919559281492738
>100	0.01427696684780762	2.0418324758778974
>500	0.0017482000221805169	0.8407804565252148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCACCGAAGCTGGCTGATCGTCGGACTGTAGAACTCTGAACC	798	0.16861090392770436	Illumina DpnII expression Sequencing Primer (100% over 27bp)
TGCACGTAAAACAGCCGACGATCAAAAATACGTGCATGTTTGA	706	0.1491720528483199	No Hit
TGCACGTAAAAAAACTCACTATAGTGAGTCGTATTACCGGCCG	655	0.13839616801083504	No Hit
AACACGTACATGGGGCCGGTAGAACTCTGAACCCCTATAGTGA	621	0.13121224478584512	No Hit
TGCACGTAAAACAGCCGACGATCAANAATACGTGCATGTTTGA	525	0.11092822626822656	No Hit
TGCACCGAAGCTGGCTGATCGTCGGNCTGTAGAACTCTGAACC	513	0.10839272395352426	Illumina DpnII expression Sequencing Primer (96% over 27bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	2.112918595585268E-4	0.0	0.0
12	0.0	0.0	6.338755786755803E-4	0.0	0.0
13	0.0	0.0	0.011621052275718973	0.0	0.0
14	2.112918595585268E-4	0.0	0.048174543979344106	0.0	0.0
15	2.112918595585268E-4	0.0	0.06655693576093594	2.112918595585268E-4	0.0
16	2.112918595585268E-4	0.0	0.08684095427855451	2.112918595585268E-4	0.0
17	2.112918595585268E-4	0.0	0.31250066028706114	2.112918595585268E-4	0.0
18	2.112918595585268E-4	0.0	0.4020884087398765	8.451674382341072E-4	0.0
19	2.112918595585268E-4	0.0	0.45470008176994964	8.451674382341072E-4	0.0
20	2.112918595585268E-4	0.0	0.49505682694562825	0.001056459297792634	0.0
21	4.225837191170536E-4	0.0	0.5273844814580828	0.001056459297792634	0.0
22	4.225837191170536E-4	0.0	0.579996154488156	0.001056459297792634	0.0
23	4.225837191170536E-4	0.0	0.6503563437211455	0.0014790430169096876	0.0
24	4.225837191170536E-4	0.0	0.7160681120438472	0.0016903348764682143	0.0
25	4.225837191170536E-4	0.0	0.847491648689251	0.0016903348764682143	0.0
26	4.225837191170536E-4	0.0	0.9421504017714709	0.001901626736026741	0.0
27	4.225837191170536E-4	0.0	1.0744191058551087	0.002112918595585268	0.0
28	4.225837191170536E-4	0.0	1.206899101798305	0.002112918595585268	0.0
29	4.225837191170536E-4	0.0	1.3702277092370463	0.002112918595585268	0.0
30	4.225837191170536E-4	0.0	1.5348240678331386	0.002112918595585268	0.0
31	4.225837191170536E-4	0.0	1.6975187996932042	0.002112918595585268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTACC	115	0.0	41.4505	25
GAACTTA	220	0.0	40.927135	26
ATAATGA	245	0.0	36.06086	21
CGTAGTC	520	0.0	35.494278	5
CGCGTAG	125	0.0	35.494278	3
CGCGTAC	95	0.0	35.494278	3
CGTCCCG	45	3.921741E-9	35.494278	5
GCGTACG	20	0.0022571515	35.494278	4
GGTAAGC	25	1.5736304E-4	35.494274	5
CGTACGG	495	0.0	35.494274	5
CGTCGCG	50	2.8012437E-10	35.494274	5
CACGTAC	9110	0.0	35.35791	3
ACGTAGT	2130	0.0	35.327637	4
ATCTACC	60	5.761285E-8	35.309685	24
CACGTAG	8090	0.0	35.29684	3
GCCCGTA	1310	0.0	35.25791	2
CGTATGG	565	0.0	35.18017	5
ACGTATA	2375	0.0	35.120655	4
CGTACCG	450	0.0	35.099895	5
ACGTACC	2240	0.0	35.098137	4
>>END_MODULE