FastQCFastQC Report
Thu 26 May 2016
SRR2099007_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099007_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122057
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCTTACATGATTCCGGTAGAACTCTGAACCCCTATAGTGA1930.15812284424490197No Hit
TAGCCTTACATGAGCCGGTAGAACTCTGAACCCCTATAGTGAG1520.12453198095971556No Hit
TAGCCTTACATGATTCCGGTAGAACNCTGAACCCCTATAGTGA1340.10978477268817027No Hit
TAGCCTTACATGATCCGGTAGAACTCTGAACCCCTATAGTGAG1310.10732690464291274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATTA150.007318277451.7788124
CTGGACG405.3873173E-639.34604623
CCCTGCG251.5685547E-435.4866264
GCCTTGA251.5685547E-435.4866263
CATAGAC251.5685547E-435.4866265
CCATACA251.5685547E-435.4866264
CATGCTC1600.035.486625
CCGTGTA301.0983302E-535.486624
CCGTGAT200.002252767335.486624
GCCATAC700.035.486623
CCATAGA405.464426E-835.486624
CCATACC301.0983302E-535.486624
CCATAAG357.730141E-735.486624
ACATGGG200.002252767335.486624
GCAATGG453.8762664E-935.486623
GCGCTGG200.002252767335.486623
GCCGTGC357.730141E-735.486623
CCCATGC200.002252767335.486623
TCGCCGT200.002281091935.3964421
AGCAATA405.5882992E-835.3964422
Produced by FastQC (version 0.11.5)