FastQCFastQC Report
Thu 26 May 2016
SRR2099006_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099006_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115022
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTTTTTATCGTAGAATGGTGGTTGCCAGGCTGGTGGGAG1580.13736502582114726No Hit
TCGACAGTACAGAATCATGAGGTCAGGAGTTTGAGACCATCCT1370.11910764897150111No Hit
TCGACAGTACCAGGGTTGATGAAAATGCTGTCACAGACTCTCA1210.10519726660986593No Hit
TCGACAGTCCGCAGCGTGGTGGCGTGCACCTGTAATCCCAGCT1190.10345846881466154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCT202.9699272E-453.5279322
CCTGCTA202.9699272E-453.5279323
TCGTAAT150.006414963453.52792720
CCATTGC452.4157453E-741.12853224
CGTAAAG351.00768106E-438.23423822
ATGCTGT351.0827745E-437.77110325
TGCCATT505.015354E-737.46955522
GCCATTG505.015354E-737.46955523
ATGCCAT505.015354E-737.46955521
AGCGTAG505.519032E-737.01568224
CATTGCA505.519032E-737.01568225
CGTAGAC505.519032E-737.01568226
TCTAGTG300.002186528635.68528421
CTAGTGA300.002186528635.68528422
TATAACC300.002186528635.68528423
TAACCTA300.002321446835.2530325
CAGCCAG358.4253406E-735.095585
GACCGTG700.035.095583
ACAGGTA251.6736134E-435.095584
GACGGTT251.6736134E-435.095583
Produced by FastQC (version 0.11.5)