FastQCFastQC Report
Thu 26 May 2016
SRR2099005_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099005_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385745
Sequences flagged as poor quality0
Sequence length43
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCC351.3814741E-436.28185325
AGACGCC200.002150942635.8428765
TAGGCCG301.0316096E-535.8428734
AGTACGC357.2041985E-735.8428735
TGCTCGT800.035.784761
TAGTCGC25150.035.7716144
TAGTCTC20650.035.756094
TAGTCGA16600.035.7349174
CTAGTCC91400.035.6467973
CTAGTCA77550.035.6117823
AGTCGCA8300.035.4110345
TAGTCAA20000.035.394844
TAGTCCC27200.035.381664
AGTCGTT3850.035.3773845
GCTCGTC11350.035.3118322
TAGTCCA25400.035.278424
AGTCCGC6300.035.2739375
TAGTCTA15350.035.2591174
TAGTCCG21350.035.2552874
AGTCGGC5750.035.219525
Produced by FastQC (version 0.11.5)