##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099005_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 385745 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.641382260301494 29.0 24.0 31.0 15.0 35.0 2 28.358039637584415 30.0 27.0 32.0 19.0 35.0 3 29.118378721694384 30.0 28.0 32.0 23.0 35.0 4 29.193264980751533 30.0 27.0 33.0 19.0 39.0 5 24.765316465540707 27.0 19.0 31.0 8.0 34.0 6 27.955986467744236 29.0 26.0 31.0 17.0 37.0 7 27.82812220508367 30.0 25.0 32.0 16.0 37.0 8 29.585529300444595 30.0 28.0 33.0 23.0 37.0 9 26.38597259847827 27.0 26.0 28.0 22.0 31.0 10 26.590781474808487 27.0 26.0 28.0 23.0 31.0 11 26.190773697650002 27.0 26.0 27.0 20.0 31.0 12 28.836630416466836 30.0 27.0 32.0 20.0 35.0 13 28.39023448132834 30.0 27.0 32.0 19.0 35.0 14 30.867961995618867 31.0 31.0 31.0 30.0 34.0 15 30.80796381028918 31.0 31.0 31.0 30.0 34.0 16 30.9588355001361 31.0 31.0 31.0 30.0 34.0 17 34.90727812414937 35.0 35.0 37.0 33.0 37.0 18 34.87331008827075 35.0 35.0 37.0 33.0 37.0 19 34.258170553085584 35.0 35.0 35.0 30.0 37.0 20 34.035432734060066 35.0 33.0 35.0 30.0 37.0 21 33.765329427471514 35.0 33.0 35.0 30.0 37.0 22 34.72583442429584 37.0 33.0 39.0 29.0 39.0 23 34.46567810341028 37.0 33.0 39.0 28.0 39.0 24 34.5736328403479 37.0 33.0 39.0 28.0 39.0 25 34.44027531141039 35.0 32.0 39.0 27.0 39.0 26 21.835842331073636 27.0 2.0 35.0 2.0 39.0 27 28.17114155724637 32.0 17.0 37.0 17.0 39.0 28 31.726132548704456 32.0 27.0 37.0 17.0 39.0 29 33.93450336362105 36.0 32.0 38.0 27.0 40.0 30 34.94595911807023 36.0 33.0 38.0 27.0 40.0 31 35.542213120066364 37.0 34.0 39.0 30.0 40.0 32 36.22935099612438 38.0 34.0 39.0 31.0 40.0 33 36.56828215530985 38.0 35.0 40.0 31.0 40.0 34 36.90978755395404 38.0 36.0 40.0 32.0 40.0 35 37.17430167597765 38.0 36.0 40.0 32.0 40.0 36 37.385529300444595 39.0 36.0 40.0 33.0 40.0 37 37.6118420199873 39.0 36.0 40.0 33.0 40.0 38 37.746786089255856 39.0 37.0 40.0 34.0 40.0 39 37.773238797651295 39.0 37.0 40.0 34.0 40.0 40 37.81793412746763 39.0 37.0 40.0 34.0 40.0 41 37.85123073533033 39.0 37.0 40.0 34.0 40.0 42 37.834618724805246 40.0 37.0 40.0 34.0 40.0 43 37.809939208544506 40.0 37.0 40.0 34.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.555504551935705 1101 2 5.747076696970478 1101 3 5.584785821954643 1101 4 8.755025747991631 1101 5 8.318195432049954 1101 6 7.002573845046246 1101 7 7.103953472719539 1101 8 6.165080767426048 1101 9 6.33815908027206 1101 10 5.974119771181268 1101 11 3.8559930798249553 1101 12 8.352799212363728 1101 13 7.156622127791234 1101 14 0.07155522603031272 1101 15 1.0364165386818094 1101 16 1.5710204369103273 1101 17 0.856967421358739 1101 18 0.4173767784155089 1101 19 0.07942021126057597 1101 20 0.03549688587804667 1101 21 -0.162033512893359 1101 22 -0.18493988393741034 1101 23 0.884389939557856 1101 24 0.22805476035830452 1101 25 0.1422041835821446 1101 26 10.601269292169107 1101 27 5.783544232089298 1101 28 2.438743018665974 1101 29 0.6187235537645179 1101 30 -0.015031391245585723 1101 31 -0.2689764458732071 1101 32 -0.35633843508785645 1101 33 -0.3576678111706144 1101 34 -0.11560305393593495 1101 35 -0.23551304137883733 1101 36 -0.31644725308064636 1101 37 -0.3672090337098055 1101 38 -0.2565028925818993 1101 39 -0.24004679238217363 1101 40 -0.22596042801619376 1101 41 -0.2691364865636885 1101 42 -0.2584461418922146 1101 43 -0.1637064234840011 1102 1 3.680368859158115 1102 2 2.290864183977579 1102 3 1.9157830043507431 1102 4 4.685148067135849 1102 5 4.534628131841838 1102 6 3.342204059293902 1102 7 3.868777228786545 1102 8 2.5750627551535388 1102 9 4.4029750105635586 1102 10 3.873844635098827 1102 11 3.96507107682908 1102 12 5.992935309418932 1102 13 4.613954928149209 1102 14 -0.17586819421026334 1102 15 1.295251303439322 1102 16 1.6276300187119261 1102 17 0.9106519166140785 1102 18 0.442473488440001 1102 19 0.18789455197146054 1102 20 0.006305769074934631 1102 21 -0.17408910382546594 1102 22 -0.17278103173513415 1102 23 0.5599589400049965 1102 24 0.04617789260384342 1102 25 -0.15707490338040486 1102 26 10.297213234937686 1102 27 5.436254174753316 1102 28 2.0958792329454496 1102 29 0.3115943372220613 1102 30 -0.09170158546444895 1102 31 -0.3584861074587451 1102 32 -0.4067029830908666 1102 33 -0.4304221951473224 1102 34 -0.24003743058693772 1102 35 -0.2786496251907806 1102 36 -0.28170957729700774 1102 37 -0.25902564299286723 1102 38 -0.16857786935622698 1102 39 -0.06531378608615768 1102 40 0.029290066615033084 1102 41 -0.1315463643159802 1102 42 -0.22367826705786342 1102 43 -0.11721449783341598 1103 1 -0.746137691024451 1103 2 -0.26271820184378214 1103 3 0.19119993283082692 1103 4 1.9461337854358085 1103 5 0.9681170103355647 1103 6 -1.6561083194083182 1103 7 -0.5529429488560744 1103 8 -0.5025297301801288 1103 9 2.4633295912674917 1103 10 1.7887197060649385 1103 11 -1.2437073393137545 1103 12 2.143422384779413 1103 13 1.6280170735746502 1103 14 -0.4725618491637995 1103 15 1.261231807317163 1103 16 1.645567958486783 1103 17 0.8657169514765712 1103 18 0.32203549336687587 1103 19 0.07852276490338994 1103 20 -0.13522913068773335 1103 21 -0.27428178757752164 1103 22 -0.21046373107721195 1103 23 0.3930163281406891 1103 24 0.028833487600948615 1103 25 0.14320494683091312 1103 26 10.191960554610027 1103 27 5.955688854387901 1103 28 2.3199453680387165 1103 29 0.6382092720151604 1103 30 -0.012051594844670888 1103 31 -0.2559848233100155 1103 32 -0.28001546419729095 1103 33 -0.37640438664646325 1103 34 -0.22303546524543805 1103 35 -0.11422206060035478 1103 36 -0.16993170102914235 1103 37 -0.20277009780516408 1103 38 -0.18363169710252691 1103 39 -0.11389229283623337 1103 40 -0.22190171352232113 1103 41 -0.10114665203083462 1103 42 -0.08020506050366549 1103 43 0.05580232185244682 1104 1 1.239530945607104 1104 2 -0.4030698921715974 1104 3 -0.5629246629299303 1104 4 1.2913676539069812 1104 5 0.381512305156928 1104 6 -0.3960650839552464 1104 7 0.004197984379079145 1104 8 -0.763459712182847 1104 9 2.163560285290963 1104 10 1.366634469957738 1104 11 1.140110498109049 1104 12 3.0301138407053116 1104 13 1.397943073088424 1104 14 0.254475875168211 1104 15 1.8517315510876386 1104 16 1.7842939468069154 1104 17 0.8802258390556474 1104 18 0.27044066133276345 1104 19 -0.06255738684045298 1104 20 -0.08267496831341958 1104 21 -0.39205991904338333 1104 22 -0.49293098529362567 1104 23 0.5046536960250734 1104 24 0.07496529626657633 1104 25 0.04749491120364269 1104 26 10.294799542774498 1104 27 5.461559396387685 1104 28 2.245138589587178 1104 29 0.45050584063832133 1104 30 -0.1929723770705678 1104 31 -0.3883732847610304 1104 32 -0.4516778778619326 1104 33 -0.5177057877759665 1104 34 -0.2100079865964375 1104 35 -0.31445800164482307 1104 36 -0.4320623763382301 1104 37 -0.26014627248199673 1104 38 -0.23419111699188733 1104 39 -0.09999818121810478 1104 40 -0.15184261457574166 1104 41 -0.34487340116901066 1104 42 -0.34165012506826287 1104 43 -0.27341326599755433 1105 1 1.0282404874313968 1105 2 -1.7012213096672788 1105 3 -1.1037186473304743 1105 4 -0.31918393172657034 1105 5 -0.31286721938652917 1105 6 -0.8626691659691659 1105 7 -5.690173133270726 1105 8 -2.217402078256363 1105 9 1.594666725905597 1105 10 0.8572346287546182 1105 11 1.1178789348938807 1105 12 3.0409176331484495 1105 13 0.6526187071083207 1105 14 0.24124716095588994 1105 15 1.8086340778262304 1105 16 1.60996206132749 1105 17 0.725053264210942 1105 18 0.3090535238800527 1105 19 -0.1136516841642603 1105 20 -0.1697367589817702 1105 21 -0.31147704108345664 1105 22 -0.5481007578404515 1105 23 0.2057260420154563 1105 24 -0.12677960121602183 1105 25 -0.016953269637980384 1105 26 10.401747373328881 1105 27 5.354142647987324 1105 28 2.169550340556306 1105 29 0.4175312014341799 1105 30 -0.2858040990826183 1105 31 -0.3614114427172197 1105 32 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0.013009027988985622 1106 26 10.380238644754286 1106 27 5.333428520686375 1106 28 2.4436009694572363 1106 29 0.5152139312127133 1106 30 -0.32393545541756197 1106 31 -0.4535522404867578 1106 32 -0.6048595347247527 1106 33 -0.6458571593257929 1106 34 -0.22769051021790432 1106 35 -0.34671684910932754 1106 36 -0.2529750456851829 1106 37 -0.31363989773374357 1106 38 -0.13579809938810428 1106 39 -3.5653494840204303E-4 1106 40 -0.09252385343112479 1106 41 -0.051618800660065745 1106 42 -0.05523142940248249 1106 43 -0.10077080097264002 1107 1 1.7654481663144708 1107 2 0.7994845270595015 1107 3 -4.624315893791593 1107 4 -24.75844319098583 1107 5 -20.756570923090234 1107 6 -9.99530054079949 1107 7 -6.096773870133244 1107 8 -2.1392907732340625 1107 9 1.2831406448507217 1107 10 1.5712388948007714 1107 11 1.9002136256055344 1107 12 3.9105433845543054 1107 13 2.3478252225242606 1107 14 0.11566639694580871 1107 15 1.729153759811883 1107 16 1.6453392185894629 1107 17 0.7395578211238742 1107 18 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0.002049766564937272 1114 6 -1.2012643128401734 1114 7 1.0895714388876385 1114 8 0.05728502263763602 1114 9 -4.710901601042039 1114 10 0.5338374384608784 1114 11 1.2417614380867263 1114 12 -22.819823107592292 1114 13 -9.57481424308325 1114 14 -0.32999166407603653 1114 15 -27.40173629254414 1114 16 -13.7618642477402 1114 17 -6.327692585086634 1114 18 -3.2895671657751215 1114 19 -1.247827929374985 1114 20 -1.246058598062227 1114 21 -0.03507857365050171 1114 22 0.10543691776619113 1114 23 1.028229234863474 1114 24 0.8415218036371073 1114 25 0.6122932182291976 1114 26 11.127060272434889 1114 27 5.454014934066507 1114 28 2.974480097899857 1114 29 1.373546527104672 1114 30 1.5173376633694957 1114 31 1.0270432188408947 1114 32 0.8247639768727382 1114 33 0.5230169151852877 1114 34 -0.12289517478040324 1114 35 0.5154111117177891 1114 36 -0.05988339647129237 1114 37 -0.23844054274913162 1114 38 -0.9658227790927398 1114 39 -0.5183353556321606 1114 40 -0.43423452076640956 1114 41 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1.8838772773653503 2215 33 2.0484684751196056 2215 34 1.5881064672557201 2215 35 1.018695183967374 2215 36 1.2882282619851892 2215 37 1.480771279910968 2215 38 0.4906632636002044 2215 39 0.6105644801642285 2215 40 1.0665864972959866 2215 41 0.2948311565683355 2215 42 0.774395031960502 2215 43 1.0211282580521654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 21.0 25 138.0 26 1105.0 27 4912.0 28 14003.0 29 27870.0 30 45052.0 31 61621.0 32 70443.0 33 65185.0 34 47976.0 35 29233.0 36 12626.0 37 5297.0 38 263.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.8337586584032082 94.43935211707723 1.2389979688557888 2.487891255663768 2 98.07644946791274 0.9469986649211266 0.2903472501263788 0.6862046170397542 3 0.6711687773010667 1.596132159846531 0.29864288584427534 97.43405617700813 4 0.4908153442432714 1.0989938417311724 98.01643084145425 0.393759972571308 5 1.282875189231485 93.20971554104743 1.6646992866342742 3.842709983086806 6 97.95951698285383 0.9311923513643834 0.37875056384837535 0.7305401019334167 7 1.3030469529168827 2.256114868119878 95.55391213547105 0.8869260434921866 8 0.4440757495236491 2.5918676846102997 0.389635640124953 96.5744209257411 9 24.067262627339154 25.33162118112508 21.159289942058898 29.441826249476865 10 24.15002726352141 25.554228442344147 20.742346740061798 29.553397554072653 11 25.016476889371692 24.468897103454022 20.419099124445452 30.095526882728834 12 26.716622460305466 24.02177141576734 18.67818637540841 30.583419748518782 13 24.12382702810413 23.373701832444947 21.236592265923445 31.265878873527477 14 32.434401765363305 29.15883342884481 17.81310694087537 20.593657864916516 15 30.494066725279755 26.604013089100565 21.351985475052153 21.549934710567523 16 21.060918511601365 29.06579510524882 30.41515894323178 19.458127439918034 17 24.062528354223645 30.455352629327663 25.903900244980495 19.578218771468197 18 22.09564304325845 32.3371488572873 24.758299736845515 20.80890836260873 19 24.25851274805895 31.562819997666853 21.787968735822886 22.39069851845131 20 22.28570687889668 30.100454963771405 24.94601355817963 22.66782459915229 21 22.15323594602652 27.91299952040856 25.803056423284815 24.130708110280107 22 21.997174299083593 27.496662302816627 26.14680682834515 24.35935656975463 23 22.1411940773264 28.228125659965116 26.798820471237907 22.83185979147058 24 22.15142127571323 29.919506409674785 26.032741837224073 21.896330477387913 25 21.484400316271113 28.880996513240614 27.082658232770353 22.55194493771792 26 17.319793995497964 32.597566447189294 27.51341215089101 22.569227406421728 27 20.700892058578773 30.822036776352803 26.874528499988333 21.602542665080094 28 21.250881413579993 30.29340702642167 27.770490273341906 20.685221286656436 29 21.5684698721156 29.786980315705698 27.808958907447785 20.835590904730918 30 22.26580322701066 29.423918453689495 27.614428221825875 20.695850097473972 31 21.803004575561577 29.632529261558805 27.489662860179653 21.07480330269997 32 21.647979883083384 29.974724234922036 27.565101297489274 20.81219458450531 33 22.172160365007972 29.425656845843758 27.787009552943005 20.615173236205266 34 21.38226030149451 30.065198511970344 27.995955877587527 20.55658530894762 35 21.991989526759905 30.198965637921425 27.9749575496766 19.83408728564207 36 22.07676055425216 30.051199626696395 28.05480304346135 19.817236775590093 37 22.126793607175724 30.241999248208014 28.02291669367069 19.608290450945574 38 22.292447082917473 29.392474302972172 28.629535055541872 19.685543558568487 39 22.166716354068104 29.497206703910617 28.52609884768435 19.809978094336934 40 22.155828332188364 29.754112172549224 28.488509248337635 19.60155024692478 41 21.831520823341847 29.74063176450764 28.22486357567823 20.20298383647228 42 22.124978936862437 29.07127765751986 29.010875059948933 19.792868345668772 43 21.963732517595822 29.16201117318436 29.116644415352113 19.75761189386771 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 15.0 9 29.0 10 102.0 11 175.0 12 175.0 13 339.5 14 504.0 15 877.5 16 1251.0 17 1386.5 18 1522.0 19 1522.0 20 1960.0 21 2398.0 22 3061.0 23 3724.0 24 4656.0 25 5588.0 26 5588.0 27 6742.0 28 7896.0 29 9166.0 30 10436.0 31 12179.0 32 13922.0 33 13922.0 34 15999.0 35 18076.0 36 20166.0 37 22256.0 38 24250.0 39 26244.0 40 26244.0 41 27259.0 42 28274.0 43 29914.5 44 31555.0 45 32864.0 46 34173.0 47 34173.0 48 34719.5 49 35266.0 50 35282.0 51 35298.0 52 33829.5 53 32361.0 54 32361.0 55 29306.5 56 26252.0 57 22884.5 58 19517.0 59 16171.0 60 12825.0 61 12825.0 62 10234.0 63 7643.0 64 5944.0 65 4245.0 66 3288.0 67 2331.0 68 2331.0 69 1736.0 70 1141.0 71 782.5 72 424.0 73 325.5 74 227.0 75 227.0 76 159.0 77 91.0 78 70.5 79 50.0 80 42.5 81 35.0 82 35.0 83 24.0 84 13.0 85 7.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.4471866129178602 2 0.0 3 0.0 4 1.7057900944924758 5 3.589417879687358 6 7.777158485528004E-4 7 0.4464088970693074 8 0.0 9 0.2706451152963745 10 0.15943174895332407 11 0.48581316672931596 12 0.5026636767812933 13 1.1256140714720866 14 0.026442338850795218 15 0.33778791688809967 16 0.05962488172238137 17 0.0 18 0.010369544647370672 19 0.0 20 0.0 21 0.0 22 0.0 23 0.5708434328377555 24 0.0 25 0.0 26 32.39808681901256 27 0.0020739089294741346 28 2.592386161842668E-4 29 0.0015554316971056008 30 2.592386161842668E-4 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 385745.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.886267553148 #Duplication Level Percentage of deduplicated Percentage of total 1 87.45257629209078 68.11354755297872 2 8.171911815095497 12.729594201025185 3 2.008326668499838 4.692632047107023 4 0.8099154687045813 2.5232517156383314 5 0.41926987092574236 1.6327682671948098 6 0.2711832823283944 1.2672872210022121 7 0.1804064374889849 0.9835828841004144 8 0.1333173437569301 0.8306872244261811 9 0.09352905787569069 0.6556166303129918 >10 0.44600672746222797 5.752968373886002 >50 0.011523480408064436 0.6087303366172182 >100 0.0020335553632715463 0.2093335457109501 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.001296193080921334 0.0 0.0 14 0.0 0.0 0.004666295091316802 0.0 0.0 15 0.0 0.0 0.008295635717896538 0.0 0.0 16 0.0 0.0 0.01218421496066054 0.0 0.0 17 0.0 0.0 0.014776601122503208 0.0 0.0 18 0.0 0.0 0.01788746451671441 0.0 0.0 19 0.0 0.0 0.02514614576987388 0.0 0.0 20 0.0 0.0 0.03136787255829628 0.0 0.0 21 0.0 0.0 0.038367315195271486 0.0 0.0 22 0.0 0.0 0.05107000738830056 0.0 0.0 23 0.0 0.0 0.0663650857431723 0.0 0.0 24 0.0 0.0 0.07621615315817444 0.0 0.0 25 0.0 0.0 0.09695524245291579 0.0 0.0 26 0.0 0.0 0.1125095594239718 0.0 0.0 27 0.0 0.0 0.1381741824262142 0.0 0.0 28 0.0 0.0 0.17161596391398462 0.0 0.0 29 0.0 0.0 0.21050175634162466 0.0 0.0 30 0.0 0.0 0.2553500369415028 0.0 0.0 31 0.0 0.0 0.29319887490440577 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCTCC 35 1.3814741E-4 36.281853 25 AGACGCC 20 0.0021509426 35.842876 5 TAGGCCG 30 1.0316096E-5 35.842873 4 AGTACGC 35 7.2041985E-7 35.842873 5 TGCTCGT 80 0.0 35.78476 1 TAGTCGC 2515 0.0 35.771614 4 TAGTCTC 2065 0.0 35.75609 4 TAGTCGA 1660 0.0 35.734917 4 CTAGTCC 9140 0.0 35.646797 3 CTAGTCA 7755 0.0 35.611782 3 AGTCGCA 830 0.0 35.411034 5 TAGTCAA 2000 0.0 35.39484 4 TAGTCCC 2720 0.0 35.38166 4 AGTCGTT 385 0.0 35.377384 5 GCTCGTC 1135 0.0 35.311832 2 TAGTCCA 2540 0.0 35.27842 4 AGTCCGC 630 0.0 35.273937 5 TAGTCTA 1535 0.0 35.259117 4 TAGTCCG 2135 0.0 35.255287 4 AGTCGGC 575 0.0 35.21952 5 >>END_MODULE