FastQCFastQC Report
Thu 26 May 2016
SRR2099004_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099004_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69874
Sequences flagged as poor quality0
Sequence length47
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGACTAGGATAAATAAATTCTTCAGCAGAAGAGCTGAGGAGAAG750.10733606205455536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCA150.004757646557.68677524
GTGAGCT150.004757646557.68677527
GACTTAG1000.053.8743255
TGCCTAG251.3220499E-553.8743254
GACTCAT503.6379788E-1253.8743255
GACTCAG550.053.8743255
GACTATC251.3220499E-553.8743255
GACTAGT456.184564E-1153.8743255
GACTAGC650.053.8743255
GACTACC550.053.8743255
AGACTAC306.130558E-753.874324
CGATTAT150.006238696653.874324
GACTTAC401.3351382E-953.874325
GACTGAC1450.053.874325
GACTCCT352.8558134E-853.874325
TGACTCG1750.053.874324
TGACTAT1750.053.874324
TGACTAG3200.053.874324
GACTACG950.053.874325
GACTACA950.053.874325
Produced by FastQC (version 0.11.5)