FastQCFastQC Report
Thu 26 May 2016
SRR2099003_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099003_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70181
Sequences flagged as poor quality0
Sequence length47
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTAAAGGTATTCGACTCACTATAGGGAGTCGTATTACCGGC820.11684074037132558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC202.7511283E-454.3190355
ACGTGGG950.054.3190355
TCCGTTG202.7511283E-454.3190354
ACGTACC401.2405508E-954.3190355
ACGTAAC750.054.3190355
CCGTTAT202.7511283E-454.3190355
TACGTGC2100.054.3190354
TACGTCG1900.054.3190354
ACGTTGG305.7903526E-754.319035
ACGTTGA700.054.319035
ACGTTCC850.054.319035
CCGTGGG150.00603848854.319035
TTCGTCA150.00603848854.319034
TACGACA150.00603848854.319034
ACGTGTT700.054.319035
ACGTGTC550.054.319035
ACGTGCT501.8189894E-1254.319035
ACGTGCG550.054.319035
ACGTGAT900.054.319035
ACGTGAG455.820766E-1154.319035
Produced by FastQC (version 0.11.5)