FastQCFastQC Report
Thu 26 May 2016
SRR2099002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133924
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGTGCCCGGCAGAGTTCCCCAACTATCACCAGACTTC2040.15232519936680505No Hit
CTACGAGTGCCCGGCAGAGTTCCCNAACTATCACCAGACTTC1460.10901705444879187No Hit
CTACGATAGACTGTTCTACAGTCCGACGATCGTTGGACTGTA1360.10155013291120336No Hit
CTACGAGTCTCGTCCGACGATCGTCCCTACGAGTTTTTTTTT1360.10155013291120336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCATG203.4253162E-452.01293619
AAGGACG203.4253162E-452.01293619
ACGGAGT203.6289965E-451.4084823
CGGAGTC250.001089761741.12678524
CTAATAC501.481385E-841.12678524
TCTAATA453.021887E-739.98437523
CTTCTAA506.29585E-736.40905421
TAATACT506.9004636E-735.9859425
CTCCGTG251.6789409E-435.080891
CTGCGTG251.6789409E-435.080891
CTGCGAG1950.035.080891
CTACGCG5550.035.080891
ATACGAG601.8189894E-1235.080891
CTACTAG650.035.0808871
CTGCGCG750.035.0808871
CTACGGG2050.035.0808871
AGTCCGC800.034.8460046
AGTCCAT358.921652E-734.8460046
AGTCAAT358.921652E-734.8460046
AGTGCCG1250.034.8460046
Produced by FastQC (version 0.11.5)