FastQCFastQC Report
Thu 26 May 2016
SRR2099001_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099001_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82855
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTCGCGGGGAAGAAAGGAGGGAAGGAAGAAAGGAGGG1710.20638464787882446No Hit
ACGATGTCGCGGGGAAGAAAGGAGNGAAGGAAGAAAGGAGGG1170.14121054854866938No Hit
ACGATGTCCCTCGGTTTCTATTATGTAGTTGCTTTAAAAAGT1160.14000362078329612No Hit
ACGATGTCCCGACGATCCTAACTACGAGTTTTTTTTTTTTTT1070.12914127089493693No Hit
ACGATGTCTGTAGCATGGGTCGGCTATGAACAGTTTATTCCG1050.12672741536419047No Hit
ACGATGTCCCGCATGTCTTTTTTTTTTTTTTTTTTTTTTTTT1030.12431355983344397No Hit
ACGATGTCTCAAAAAATAAAACCTTAAAATAAAATAAAAAAA970.11707199324120451No Hit
ACGATGTCCCTCGTTTCTATTATGTAGTTGCTTTAAAAAGTT970.11707199324120451No Hit
ACGACGTCGACGGTGTCCTCGTCCGGCGGCACGAGGCGCCCA950.11465813771045802No Hit
ACGATGTCCCTCGGTTTCTATTATNTAGTTGCTTTAAAAAGT860.10379578782209885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGC150.00799273550.61453624
CCGGCGG150.00799273550.61453623
AAAAACG150.00799273550.61453623
AGTCTTT150.00799273550.61453624
GAAGTAA150.00799273550.61453624
ATCGCGG203.9058676E-450.61453623
GATCGCG250.001110218540.94362622
AGTTTCT250.001115317340.90557521
ACCTTAA250.001115317340.90557521
ATCGTGC250.001172640240.49162723
CCGGGAA351.3147335E-436.52283521
CTTAAAA351.3959927E-436.1532423
CCGCTGT601.8189894E-1234.9320641
ACGATGG406.231494E-834.9320641
ACGATAT1250.034.9320641
ACGCTGT2550.034.9320641
ACGAGGT200.002428859234.9320641
ACGAAGC200.002428859234.9320641
ACGAAGT503.237801E-1034.932061
GTCATCC359.137384E-734.7116746
Produced by FastQC (version 0.11.5)