FastQCFastQC Report
Thu 26 May 2016
SRR2099000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124309
Sequences flagged as poor quality0
Sequence length42
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCATTAACGATCGATTTGACGATCTTTTTTTTTTTT3480.2799475500567135No Hit
TGACGATCCTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.2485741177227715No Hit
TGACGTACAGGGGCAAGACCGCCATGCTGATCTCGGTGGTGA2970.2389207539277124No Hit
TGACGATCGCTGCAGTCCGACGATCGATTTGACGATCTTTTT2920.23489851901310443No Hit
TGACGATCGCTGCAGTCCGACGATNGATTTGACGATCTTTTT2120.17054276037937718No Hit
TGACGATCATTAACGATCGATTTGNCGATCTTTTTTTTTTTT2100.168933866413534No Hit
TGACGTACAGGGGCAAGACCGCCANGCTGATCTCGGTGGTGA2060.16571607848184763No Hit
TGACGATCGCTGGGAAGAATGGGGCTCTTCTCATCCCCTTTC1980.15928050261847493No Hit
TGACGATCCTAATCTTTTTTTTTTNTTTTTTTTTTTTTTTTT1760.14158266899419994No Hit
TGACGATAAAGTTAGCACCACCACCATCTTCCAGTGTTTTTT1620.13032041123329768No Hit
TGACGATCGCTGGGAAGAATGGGGNTCTTCTCATCCCCTTTC1330.10699144872857154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATGT150.00795303150.70122520
AGGCTCG150.00795303150.70122522
TTTGTCA150.00828627250.177924
CAAGGTC150.00828627250.177925
CTGTCGG250.001166243740.5609821
TCGACTT250.001227369940.1423224
TGGCGCT200.002379622835.0925671
TGGCGAG251.6749848E-435.0925671
TGACGTA850.035.0925671
TGACGGT1550.035.0925671
TGCCGGT251.6749848E-435.0925671
ATCCTGC454.658432E-934.826386
ATCTACG454.658432E-934.826386
ATCAGCG454.658432E-934.826386
ATCACTG406.44668E-834.826386
ATCAACA200.002470255134.826386
ATCCCGT800.034.826386
ATCCACG900.034.826386
ACCCGCG200.002470255134.826386
ATCATAC200.002470255134.826386
Produced by FastQC (version 0.11.5)