##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099000_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124309 Sequences flagged as poor quality 0 Sequence length 42 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.784384075167527 30.0 26.0 31.0 17.0 35.0 2 28.00278338656091 30.0 27.0 31.0 17.0 35.0 3 25.04000514846069 28.0 19.0 31.0 12.0 33.0 4 28.204128421916355 30.0 27.0 31.0 19.0 35.0 5 27.39857934662816 30.0 25.0 31.0 14.0 37.0 6 21.40604461462967 24.0 16.0 28.0 7.0 32.0 7 26.121511716770307 28.0 23.0 31.0 15.0 34.0 8 27.94605378532528 30.0 26.0 31.0 17.0 37.0 9 26.035637001343428 27.0 26.0 28.0 22.0 31.0 10 26.088263922966156 27.0 26.0 28.0 20.0 31.0 11 25.606102534812443 27.0 26.0 27.0 18.0 31.0 12 27.983846704582934 30.0 26.0 31.0 18.0 35.0 13 31.00583224062618 31.0 31.0 31.0 30.0 34.0 14 30.935322462573104 31.0 31.0 33.0 30.0 34.0 15 31.114585428247352 31.0 31.0 33.0 30.0 34.0 16 35.01828507992181 35.0 35.0 37.0 33.0 37.0 17 35.05890965255935 35.0 35.0 37.0 33.0 37.0 18 34.581703657820434 35.0 35.0 37.0 32.0 37.0 19 34.393583730864215 35.0 35.0 37.0 30.0 37.0 20 34.17842634081201 35.0 33.0 37.0 30.0 37.0 21 35.32931646139861 37.0 34.0 39.0 30.0 39.0 22 35.07282658536389 37.0 34.0 39.0 30.0 39.0 23 35.235638610237395 37.0 34.0 39.0 30.0 39.0 24 35.26482394677779 37.0 34.0 39.0 30.0 39.0 25 22.052393631997685 30.0 2.0 37.0 2.0 39.0 26 28.69272538593344 32.0 17.0 38.0 17.0 40.0 27 32.73397742721766 34.0 30.0 38.0 25.0 40.0 28 34.95556234866341 36.0 33.0 38.0 27.0 40.0 29 35.97078248558029 37.0 34.0 39.0 31.0 40.0 30 36.50650395385692 38.0 35.0 40.0 31.0 40.0 31 37.098979156778675 38.0 36.0 40.0 32.0 41.0 32 37.367133514065756 39.0 36.0 40.0 32.0 41.0 33 37.56130288233354 39.0 36.0 40.0 33.0 41.0 34 37.71276416027802 39.0 37.0 40.0 33.0 41.0 35 37.80040865906732 39.0 37.0 40.0 34.0 41.0 36 37.940865102285436 39.0 37.0 40.0 34.0 41.0 37 37.95377647636133 39.0 37.0 40.0 34.0 41.0 38 37.900095729190966 39.0 37.0 40.0 34.0 41.0 39 37.82909523847831 39.0 37.0 40.0 34.0 41.0 40 37.80106026112349 39.0 37.0 40.0 34.0 41.0 41 37.74380776934896 39.0 37.0 40.0 33.0 41.0 42 37.65071716448527 40.0 37.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.72626095027589 1101 2 6.126383654858419 1101 3 5.726864642687811 1101 4 5.178918936106751 1101 5 8.675238572632622 1101 6 7.5076440386433845 1101 7 6.754354359004658 1101 8 6.473023409849283 1101 9 6.073298849934908 1101 10 5.50862157286139 1101 11 3.105393375191479 1101 12 7.8486752101551005 1101 13 0.07827977694454802 1101 14 1.2406515951479236 1101 15 1.7652584043093071 1101 16 1.0198150374811945 1101 17 0.4303669869692115 1101 18 0.09537489903189567 1101 19 0.14985808214720464 1101 20 0.04771133275659878 1101 21 -0.2662349112955127 1101 22 0.3085998840968216 1101 23 0.18752191974218846 1101 24 -0.24490866243987597 1101 25 10.714005651784053 1101 26 5.59469439790017 1101 27 2.4180552539784017 1101 28 0.9960297315059705 1101 29 0.2626715568364659 1101 30 -0.11045143243121203 1101 31 0.017082631548660743 1101 32 -0.30494605856963375 1101 33 0.03778691875379536 1101 34 -0.1651630028882778 1101 35 0.09393580950596458 1101 36 0.10834376418289793 1101 37 -0.0722663388330318 1101 38 -0.031217080830558075 1101 39 0.03700318830372851 1101 40 -0.048199656577253336 1101 41 -0.15912130136118208 1101 42 -0.0544060049302999 1102 1 3.7330106226218938 1102 2 2.885082868488823 1102 3 2.2363993740115333 1102 4 1.3686469832889259 1102 5 4.4417031860009075 1102 6 3.795242334842598 1102 7 2.766444791507933 1102 8 3.6914539472018397 1102 9 4.402328994102664 1102 10 3.749004928038321 1102 11 3.9435978312858424 1102 12 5.850706664938848 1102 13 -0.0011435520200642202 1102 14 1.5357367851872432 1102 15 1.7082957568650095 1102 16 0.9820201488835565 1102 17 0.39904326481980235 1102 18 0.14765209431367765 1102 19 0.0034950415181143057 1102 20 -0.0659682216339661 1102 21 -0.16402324681320835 1102 22 0.3622368434677199 1102 23 -0.055613729012726765 1102 24 -0.1778051237767002 1102 25 10.554503685860073 1102 26 5.690926376930314 1102 27 2.329801649784436 1102 28 0.8411476869450283 1102 29 0.11596447951011868 1102 30 -0.10694553465926049 1102 31 -0.21469980619708195 1102 32 -0.5603523495263829 1102 33 -0.5451586906826407 1102 34 -0.26037925452654775 1102 35 -0.460241778960615 1102 36 -0.5251260916493408 1102 37 -0.5756903231056398 1102 38 -0.5097885094019858 1102 39 -0.42462852860321476 1102 40 -0.6397134180451403 1102 41 -0.3564673039824129 1102 42 -0.37262356718456147 1103 1 1.8012293447133132 1103 2 -2.359156800261683 1103 3 -1.1881479995372217 1103 4 -0.055388902072767365 1103 5 2.2031304816086035 1103 6 1.1592717251162057 1103 7 -1.9588251605907914 1103 8 -0.7236105977360516 1103 9 2.6425782431081046 1103 10 1.9499142403708696 1103 11 -1.6447962581839946 1103 12 2.527373060976842 1103 13 -0.6156519550402813 1103 14 1.4550637316839534 1103 15 1.6782640933105704 1103 16 0.861613393991945 1103 17 0.2619725495482257 1103 18 0.26602913417728047 1103 19 -0.05352371304874737 1103 20 0.45594458180843134 1103 21 0.273319450272119 1103 22 0.6344216287238709 1103 23 0.29357438497606836 1103 24 0.2991494917952764 1103 25 11.397444336992653 1103 26 5.950035737976023 1103 27 2.5386146724360543 1103 28 0.7124962296096342 1103 29 -0.02334298172232252 1103 30 -0.25125421372072054 1103 31 0.017714742800237104 1103 32 -0.5496679296189342 1103 33 0.03982547754014121 1103 34 -0.16168639100458648 1103 35 -0.14675635231451878 1103 36 -0.39770870105098055 1103 37 -0.29977898105806133 1103 38 0.11233538440332325 1103 39 0.07717385834165214 1103 40 0.052906538113013823 1103 41 0.1740961449093561 1103 42 0.15381144134023828 1104 1 1.4649857443684802 1104 2 1.1062366795673064 1104 3 -0.0917309267526889 1104 4 -0.4188409332485463 1104 5 1.385672398250346 1104 6 0.40548911106599306 1104 7 -0.4646530252611747 1104 8 0.34453363421736327 1104 9 2.0359657059417238 1104 10 1.1699216296634347 1104 11 0.38992901847463557 1104 12 2.680498555795541 1104 13 0.17671772071052416 1104 14 2.024193202645794 1104 15 1.7927541441559427 1104 16 0.7711605359191438 1104 17 0.3554734908033552 1104 18 -0.0034997104540437363 1104 19 -0.20306012290817677 1104 20 -0.23067903929679545 1104 21 -0.14122426091210372 1104 22 0.6590614007114155 1104 23 0.30176858262205286 1104 24 -0.03343464937540119 1104 25 10.634227179759062 1104 26 5.70462765549744 1104 27 2.1505140724958736 1104 28 0.5102231424687673 1104 29 -0.08768700607180335 1104 30 -0.5763347042289979 1104 31 -0.05214486064105728 1104 32 -0.14698667203171567 1104 33 -0.06296925846858414 1104 34 -0.18781281828162832 1104 35 -0.21322479827591678 1104 36 -0.1897001153967679 1104 37 -0.53387880688188 1104 38 -0.42271097461073026 1104 39 0.10332667594928324 1104 40 -0.2824441893804348 1104 41 -0.04322382905218092 1104 42 0.06919524471468463 1105 1 -0.05765568987682812 1105 2 1.056526034750238 1105 3 -2.640722057916708 1105 4 -0.9961963995598104 1105 5 -1.7030425282264297 1105 6 -0.817781510195303 1105 7 -1.2319246737665814 1105 8 -3.3845927212356663 1105 9 1.7979727283949778 1105 10 0.6664524669116574 1105 11 0.760356785945536 1105 12 3.053113644771706 1105 13 0.21789161277019176 1105 14 1.7798020883073242 1105 15 1.7043890120089564 1105 16 0.975657385556282 1105 17 0.4220628189768476 1105 18 0.14622997380122627 1105 19 0.21782658357896167 1105 20 0.35891480078077365 1105 21 0.13425427101437748 1105 22 0.5578601449940521 1105 23 0.11721886533557324 1105 24 0.055377688180556106 1105 25 11.30939222512163 1105 26 6.078221283041749 1105 27 2.9852561766637393 1105 28 1.2133141731222636 1105 29 0.5257681206290172 1105 30 0.33573851348033656 1105 31 -0.038994364951498994 1105 32 -0.26453085017001143 1105 33 0.1286117676242995 1105 34 0.06288344954570846 1105 35 0.017325087266065964 1105 36 0.11550650678550767 1105 37 -0.21504791694311365 1105 38 -0.17927625995877605 1105 39 -0.11792045153082142 1105 40 -0.4022642445505227 1105 41 -0.06534942735545712 1105 42 0.0440986084963626 1106 1 -3.6348177669252735 1106 2 -3.779046374296101 1106 3 -1.3965318587699151 1106 4 -5.848449868557971 1106 5 -0.5199144885918656 1106 6 -0.14195508672104395 1106 7 -1.175310364027414 1106 8 -3.5079897096842423 1106 9 0.12475657588242939 1106 10 0.5467046632403978 1106 11 0.7950435209640752 1106 12 3.375060049805242 1106 13 0.21763837752763848 1106 14 1.6941501374219747 1106 15 1.8547263343384621 1106 16 0.8367611016211427 1106 17 0.23182471291673323 1106 18 0.16487198357999944 1106 19 -0.14569585371285143 1106 20 0.17631920447671234 1106 21 -0.08041129613516773 1106 22 0.4993433243300558 1106 23 0.38879742994627264 1106 24 0.29678229965925595 1106 25 11.184268042454608 1106 26 6.054534048045937 1106 27 2.484272455144584 1106 28 1.4914378947712805 1106 29 0.402358145757745 1106 30 0.30172057923059725 1106 31 0.03093102805011938 1106 32 -0.09787608772415268 1106 33 0.16158429484708847 1106 34 2.888921554600188E-4 1106 35 -0.0978644820392276 1106 36 -0.21669267896579214 1106 37 -0.1940228985997905 1106 38 -0.2742199962824543 1106 39 -0.21933791956799098 1106 40 -0.35381190147521124 1106 41 -0.18033121389763096 1106 42 -0.29755469297695214 1107 1 3.136680347392538 1107 2 2.0997617675713904 1107 3 0.26575717217667005 1107 4 -3.431353016711075 1107 5 -23.172975713081662 1107 6 -17.608427389928043 1107 7 -8.195023098400323 1107 8 -5.45716990600917 1107 9 1.3729711959375273 1107 10 1.5765278638181393 1107 11 1.925249207432632 1107 12 4.236027765856278 1107 13 0.285094980090026 1107 14 1.9687643081230206 1107 15 1.829396674296202 1107 16 0.31596510301199743 1107 17 -0.5330668269233172 1107 18 -0.12207267632852137 1107 19 -0.3965049584818914 1107 20 0.08816021873300173 1107 21 -0.24659205415265717 1107 22 0.12370473337598042 1107 23 0.360900032455163 1107 24 0.21485542668201418 1107 25 11.03156790604356 1107 26 6.1257887622514104 1107 27 2.3151227507018675 1107 28 0.21545961355052867 1107 29 -0.02348506177428078 1107 30 0.09121960295541953 1107 31 -0.44589246674753724 1107 32 0.11120728350114462 1107 33 0.14291470381277094 1107 34 0.4387033142807937 1107 35 -1.0804252651991462 1107 36 -0.14163985311723337 1107 37 0.5967867411145491 1107 38 0.09021149059801559 1107 39 0.3129861502958633 1107 40 0.07588291223008525 1107 41 0.19766113638455352 1107 42 0.08792689153103339 1113 1 -8.652310478295536 1113 2 -3.573021107046344 1113 3 0.38043607125923984 1113 4 0.9187998884877047 1113 5 3.8270242869183377 1113 6 -17.608427389928043 1113 7 -9.584931355281057 1113 8 -3.4632861139602404 1113 9 -20.948129721493665 1113 10 -21.625306998567183 1113 11 -10.449368529570426 1113 12 -22.15846764698776 1113 13 -0.2026726040078337 1113 14 0.019223023719352028 1113 15 -0.7317654052145031 1113 16 0.12636265652881917 1113 17 0.30179555839778516 1113 18 1.1959701371271336 1113 19 0.33438189167101484 1113 20 -1.3552648577195967 1113 21 -0.41325872081932147 1113 22 2.593123693620626 1113 23 2.188117157837425 1113 24 0.11699609946489176 1113 25 11.242577080355485 1113 26 8.431599159804932 1113 27 4.191269539692691 1113 28 1.7567440172202566 1113 29 1.9596953663602719 1113 30 1.062167615187839 1113 31 1.1718445363105658 1113 32 1.7748158461922756 1113 33 1.7514773949442741 1113 34 0.7276941399688681 1113 35 0.9623881904583413 1113 36 1.2910818594209914 1113 37 1.852138423071736 1113 38 0.7568781572646799 1113 39 -2.2237110974105576 1113 40 1.0101336767560838 1113 41 0.01111679387690856 1113 42 -1.4258345763588736 1114 1 -9.034663419472007 1114 2 -0.9749818913600699 1114 3 -1.9136815757995826 1114 4 -7.865513837002492 1114 5 -1.7023874777875463 1114 6 -0.02019209581039405 1114 7 -0.2614019435163506 1114 8 1.762204082118192 1114 9 -7.271659133258371 1114 10 0.5511635896681106 1114 11 1.442788333174672 1114 12 -22.15846764698776 1114 13 -0.09482946675293036 1114 14 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4 5.915896288023131 1201 5 9.422843102249345 1201 6 7.740004665821086 1201 7 7.150260282349606 1201 8 7.1842167896402245 1201 9 6.905528815091703 1201 10 6.1638916077045955 1201 11 5.979783619093919 1201 12 9.18996440876137 1201 13 0.22009162363444545 1201 14 1.9721846961932208 1201 15 1.9349012614521648 1201 16 1.0996495322547162 1201 17 0.4829802273873298 1201 18 0.34986096174966974 1201 19 0.17991034695557317 1201 20 0.35960157665903836 1201 21 0.1628156113525705 1201 22 0.8079901279992612 1201 23 0.3123912577212806 1201 24 0.2453352399991502 1201 25 -4.368921177484236 1201 26 -2.622988528929099 1201 27 -1.4341660003769974 1201 28 -0.7362873416647631 1201 29 -0.3080165964736423 1201 30 -0.28336084009671936 1201 31 -0.45591388413078704 1201 32 -0.4348241073501171 1201 33 -0.5226224889117077 1201 34 -0.22526753250500064 1201 35 -0.03528892916999382 1201 36 -0.02715275149654417 1201 37 -0.256456234303414 1201 38 -0.059614293374110616 1201 39 0.04109726495877908 1201 40 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41 -0.7111054283453129 2209 42 -1.1758345763588736 2215 1 -12.498464324449383 2215 2 -4.855072389097629 2215 3 -1.4272562364330668 2215 4 0.8290562987441135 2215 5 2.1347165946106443 2215 6 6.083880302379651 2215 7 3.2227609524112495 2215 8 1.8956882450141208 2215 9 -20.948129721493665 2215 10 -9.240691613951798 2215 11 -0.6929582731601691 2215 12 -22.15846764698776 2215 13 0.5793786780434509 2215 14 -27.480776976280648 2215 15 -12.872791046240142 2215 16 -4.668509138342976 2215 17 -2.5443582877560615 2215 18 -0.39377345261645047 2215 19 0.5138690711581972 2215 20 -0.8680853705401077 2215 21 1.1380233304627296 2215 22 2.8110724115693486 2215 23 1.4829889527092206 2215 24 1.783662766131556 2215 25 10.319500157278565 2215 26 6.867496595702363 2215 27 2.6143464627696105 2215 28 1.1413594018356434 2215 29 1.9468748535397609 2215 30 1.6903727433929632 2215 31 -0.46918110471507646 2215 32 0.9030209743973998 2215 33 0.4950671385340186 2215 34 0.22769413996886811 2215 35 0.9111061391762902 2215 36 -1.490969422630286 2215 37 0.09572816666148043 2215 38 0.09021149059801559 2215 39 -2.7237110974105576 2215 40 -0.6693535027310986 2215 41 0.9598347425948575 2215 42 1.0357038851795863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 3.0 25 78.0 26 415.0 27 1604.0 28 4294.0 29 8427.0 30 13959.0 31 19067.0 32 22161.0 33 21726.0 34 16278.0 35 10192.0 36 4298.0 37 1754.0 38 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8580546419616398 0.9793545866032758 97.21254355400697 0.9500472174281155 2 94.47858721791727 3.6185941814236324 1.1238775394531217 0.7789410612059822 3 2.6490439147607976 92.09872173374414 1.2123016032628369 4.039932748232228 4 0.8044469829215907 1.8059834766589709 0.5824196156352316 96.80714992478421 5 94.9287390532883 3.3304169358201756 1.0687097802889687 0.6721342306025496 6 2.1954491297356293 89.89926762816506 2.7620166470867598 5.143266595012539 7 0.8366248622384541 2.0095085633381333 95.75010658922524 1.4037599851981755 8 1.21455522987482 2.962705057407977 0.7287331379248919 95.09400657479232 9 25.748411239072226 26.855705022742672 24.98790283557534 22.40798090260976 10 32.87392060832581 22.787247067921122 24.2267044722258 20.11212785152726 11 29.823126386886734 22.80568198383518 24.968010495796822 22.40318113348127 12 28.922151330632445 24.98160322812806 25.382693287402052 20.713552153837444 13 30.80723948399765 27.23980589556022 19.93191859201854 22.021036028423584 14 29.2307319594289 26.76933264422766 20.889592350927064 23.11034304541637 15 24.78386167146974 26.37209601854684 28.86915782525397 19.974884484729444 16 21.497236724613664 29.32691920938951 28.537756719143424 20.638087346853407 17 21.674068352963893 27.38302117525906 26.04910857951986 24.89380189225719 18 22.03219396825652 29.34300814904794 22.601742432165008 26.02305545053053 19 24.941878705483916 28.13553322768263 24.602402078691004 22.320185988142452 20 21.831082222526124 27.81214554054815 26.95782284468542 23.398949392240304 21 22.057936271710012 24.643428874820007 26.571688292883056 26.726946560586924 22 23.637305951233735 25.524954043745495 27.719517034181735 23.118222970839035 23 22.822965352468447 27.568398104722906 28.179777811743318 21.42885873106533 24 22.342710503664257 26.810609046810768 29.016402673981773 21.830277775543202 25 17.384071785033452 30.237866847991064 29.079495367728303 23.29856599924718 26 22.870175212781362 27.47333193892491 28.550512445095166 21.105980403198558 27 23.2078644002349 27.378989115657205 28.762660188082727 20.650486296025164 28 20.32274412954814 29.537684318914962 29.97610792460723 20.16346362692967 29 21.07587604981176 27.358657528075426 30.77356244167713 20.79190398043569 30 21.716046303968337 27.915919201345034 29.194185457207446 21.173849037479183 31 22.286399214859745 28.930326846809162 29.527226508136984 19.256047430194112 32 20.72979430290647 29.62617348703634 29.207056608934188 20.43697560112301 33 20.78208335679637 28.406631860927206 29.625369040053418 21.185915742223006 34 23.37240264180389 27.64160278016877 29.469306325366627 19.516688252660707 35 21.014568534860707 29.812805187074147 29.886814309502935 19.28581196856221 36 20.73542543178692 28.55384565880186 30.788599377358032 19.92212953205319 37 21.528610156947607 26.956213950719576 30.41694487124826 21.098231021084555 38 22.630702523550188 27.51932683876469 30.523131872993908 19.32683876469121 39 22.008865005751797 27.836278950035798 32.09180348969101 18.063052554521395 40 21.341978456909796 27.9183325422938 30.975231077395843 19.764457923400556 41 23.485829666395837 28.20552011519681 30.025179190565442 18.28347102784191 42 22.8398587391098 29.497461969768885 29.547337682710022 18.1153416084113 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 15.0 9 28.0 10 28.0 11 130.5 12 233.0 13 513.0 14 793.0 15 609.0 16 425.0 17 425.0 18 555.0 19 685.0 20 903.0 21 1121.0 22 1121.0 23 1333.0 24 1545.0 25 1985.0 26 2425.0 27 2981.0 28 3537.0 29 3537.0 30 3699.5 31 3862.0 32 4552.5 33 5243.0 34 5243.0 35 5962.0 36 6681.0 37 7185.0 38 7689.0 39 8116.5 40 8544.0 41 8544.0 42 8760.0 43 8976.0 44 9542.0 45 10108.0 46 10876.5 47 11645.0 48 11645.0 49 11828.5 50 12012.0 51 11785.0 52 11558.0 53 11558.0 54 10419.5 55 9281.0 56 7998.5 57 6716.0 58 5742.0 59 4768.0 60 4768.0 61 3698.0 62 2628.0 63 2029.0 64 1430.0 65 1227.0 66 1024.0 67 1024.0 68 888.0 69 752.0 70 554.5 71 357.0 72 357.0 73 254.0 74 151.0 75 104.5 76 58.0 77 40.5 78 23.0 79 23.0 80 14.5 81 6.0 82 4.0 83 2.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 1.184950405843503 2 0.6499931622006452 3 0.0 4 0.0 5 1.6185473296382404 6 3.44946866276778 7 0.0 8 0.6491887152177236 9 0.25179190565445786 10 0.13273375218206246 11 0.6684954428078417 12 0.5196727509673476 13 0.03700456121439317 14 0.3837212108535987 15 0.06676909958249201 16 0.0 17 0.010457810777980676 18 0.0 19 0.0 20 0.0 21 0.0 22 0.6612554199615474 23 0.0 24 0.0 25 33.74815982752657 26 0.0024133409487647715 27 0.001608893965843181 28 0.0 29 8.044469829215905E-4 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 124309.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.71637612723133 #Duplication Level Percentage of deduplicated Percentage of total 1 85.75000276570088 62.354294540218326 2 8.95202062106579 13.01916997160302 3 2.4570486652727532 5.3600302472065575 4 0.9502948237142256 2.764079833318585 5 0.5011450001659421 1.8220724163174027 6 0.30754593829168186 1.341817567513213 7 0.20687442611706658 1.0530211006443622 8 0.15487924949940812 0.9009806208721814 9 0.09514011040677929 0.6226419647813111 >10 0.5907537088048853 7.583521708001833 >50 0.021019326717776822 1.0481944187468326 >100 0.013275364242806412 2.1301756107763716 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGACGATCATTAACGATCGATTTGACGATCTTTTTTTTTTTT 348 0.2799475500567135 No Hit TGACGATCCTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.2485741177227715 No Hit TGACGTACAGGGGCAAGACCGCCATGCTGATCTCGGTGGTGA 297 0.2389207539277124 No Hit TGACGATCGCTGCAGTCCGACGATCGATTTGACGATCTTTTT 292 0.23489851901310443 No Hit TGACGATCGCTGCAGTCCGACGATNGATTTGACGATCTTTTT 212 0.17054276037937718 No Hit TGACGATCATTAACGATCGATTTGNCGATCTTTTTTTTTTTT 210 0.168933866413534 No Hit TGACGTACAGGGGCAAGACCGCCANGCTGATCTCGGTGGTGA 206 0.16571607848184763 No Hit TGACGATCGCTGGGAAGAATGGGGCTCTTCTCATCCCCTTTC 198 0.15928050261847493 No Hit TGACGATCCTAATCTTTTTTTTTTNTTTTTTTTTTTTTTTTT 176 0.14158266899419994 No Hit TGACGATAAAGTTAGCACCACCACCATCTTCCAGTGTTTTTT 162 0.13032041123329768 No Hit TGACGATCGCTGGGAAGAATGGGGNTCTTCTCATCCCCTTTC 133 0.10699144872857154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 8.044469829215906E-4 0.0 0.0 0.0 0.0 5 8.044469829215906E-4 0.0 0.0 0.0 0.0 6 8.044469829215906E-4 0.0 0.0 0.0 0.0 7 8.044469829215906E-4 0.0 0.0 0.0 0.0 8 8.044469829215906E-4 0.0 0.0 0.0 0.0 9 8.044469829215906E-4 0.0 0.0 0.0 0.0 10 8.044469829215906E-4 0.0 8.044469829215906E-4 0.0 0.0 11 8.044469829215906E-4 0.0 0.0032177879316863623 0.0 0.0 12 8.044469829215906E-4 0.0 0.004022234914607953 0.0 0.0 13 8.044469829215906E-4 0.0 0.017697833624274992 0.0 0.0 14 8.044469829215906E-4 0.0 0.02735119741933408 0.0 0.0 15 8.044469829215906E-4 0.0 0.08124914527508065 0.0 0.0 16 8.044469829215906E-4 0.0 0.11181813062610109 0.0 0.0 17 8.044469829215906E-4 0.0 0.16571607848184766 0.0 0.0 18 8.044469829215906E-4 0.0 0.2373118599618692 0.0 0.0 19 8.044469829215906E-4 0.0 0.36361003628055893 0.0 0.0 20 8.044469829215906E-4 0.0 0.4022234914607953 0.0 0.0 21 8.044469829215906E-4 0.0 0.4319880298288941 0.0 0.0 22 8.044469829215906E-4 0.0 0.47221037897497364 0.0 0.0 23 8.044469829215906E-4 0.0 0.5212816449331906 0.0 0.0 24 8.044469829215906E-4 0.0 0.6242508587471542 0.0 0.0 25 8.044469829215906E-4 0.0 0.7280245195440395 0.0 0.0 26 8.044469829215906E-4 0.0 0.9178740075135349 0.0 0.0 27 8.044469829215906E-4 0.0 1.209888262314072 0.0 0.0 28 8.044469829215906E-4 0.0 1.42628450071998 0.0 0.0 29 8.044469829215906E-4 0.0 1.7697833624274992 0.0 0.0 30 8.044469829215906E-4 0.0 2.345767402199358 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATGT 15 0.007953031 50.701225 20 AGGCTCG 15 0.007953031 50.701225 22 TTTGTCA 15 0.008286272 50.1779 24 CAAGGTC 15 0.008286272 50.1779 25 CTGTCGG 25 0.0011662437 40.56098 21 TCGACTT 25 0.0012273699 40.14232 24 TGGCGCT 20 0.0023796228 35.092567 1 TGGCGAG 25 1.6749848E-4 35.092567 1 TGACGTA 85 0.0 35.092567 1 TGACGGT 155 0.0 35.092567 1 TGCCGGT 25 1.6749848E-4 35.092567 1 ATCCTGC 45 4.658432E-9 34.82638 6 ATCTACG 45 4.658432E-9 34.82638 6 ATCAGCG 45 4.658432E-9 34.82638 6 ATCACTG 40 6.44668E-8 34.82638 6 ATCAACA 20 0.0024702551 34.82638 6 ATCCCGT 80 0.0 34.82638 6 ATCCACG 90 0.0 34.82638 6 ACCCGCG 20 0.0024702551 34.82638 6 ATCATAC 20 0.0024702551 34.82638 6 >>END_MODULE