FastQCFastQC Report
Thu 26 May 2016
SRR2098999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2098999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342891
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCACGGGATCTGCATTTTTTTTTTTTTTTTTTTTTTTT5680.16565030869868266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCG404.8945894E-639.94613623
CGCACGG251.5427318E-435.610525
TGCAGCG7600.035.3762365
TGCACGA4700.035.231685
TCTGCAG91100.035.1609883
CTGCACG19800.035.1608924
TGCACGG6850.035.0906565
CAGCGTA750.034.9917647
CAACGGT601.8189894E-1234.9917647
CACTACG200.00241977534.9917647
CATGTCG200.00241977534.9917647
CAAGACG800.034.9917647
CACCGTT200.00241977534.9917647
CACGTAA200.00241977534.9917647
CAGTAGC406.2413164E-834.9917647
CACCGGG301.2159258E-534.9917647
CAACCGG358.6913496E-734.991767
CACGATT1400.034.991767
CACGAAT358.6913496E-734.991767
CAAGCGG650.034.991767
Produced by FastQC (version 0.11.5)