FastQCFastQC Report
Thu 26 May 2016
SRR2098998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2098998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164555
Sequences flagged as poor quality0
Sequence length43
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCATGGTCGTCAGTCCGACGATCTCCAGTAGCCATGTTTT2220.1349093008416639No Hit
TAGCCATGGCGGGGCCTAGGTGACAGAGCAAGACCCTGCCTCA1940.1178937133481207No Hit
TAGCCATGTTGCTTACAGTCCGACGATCGGACCTAGCCATGTT1730.10513202272796329No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATC259.2134485E-442.56303423
TCACCCA656.002665E-1140.92599521
GATCTCC800.039.90284323
AGAGCAA601.371518E-939.4540825
CCCATAG701.4915713E-1037.5753124
CTCCAGT850.037.13324726
CAGAGCA653.0031515E-936.41914724
ATCTCCA950.035.99319524
CCGTACG200.002250047935.502854
CATAGAT200.002250047935.502855
CATGGCG4750.035.5028465
CCGTGTA502.764864E-1035.5028464
CCTGTCG251.5667724E-435.5028465
CCATAGG301.0972726E-535.5028464
CATACGG750.035.5028465
GCCGTGT1100.035.5028463
GCCGTGA251.5667724E-435.5028463
CCATATG453.8780854E-935.5028424
ACCTGAA300.002257141335.46919320
TAGCTTA300.002257141335.46919321
Produced by FastQC (version 0.11.5)