FastQCFastQC Report
Thu 26 May 2016
SRR2098997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2098997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208594
Sequences flagged as poor quality0
Sequence length43
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAGCTGACCTGGTGGAAGGAAGGTGACCAGCCCACAGGTC3520.16874886142458556No Hit
CGTAAGCTTTGATCAGTCCGACGATCCAGGCCGTAAGCTTTTT2530.12128824414892087No Hit
CGTAAGCTGGACTGGTGGGGGATGGGGGAGGGATAGCATTAGG2460.11793244292740922No Hit
TGTAAGCTGACCTGGTGGAAGGAAGNTGACCAGCCCACAGGTC2390.11457664170589758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTC250.001016965841.72630326
GGCATAT405.27705E-639.49454522
CCGCTGT200.002186942435.713824
CCGCTAG200.002186942435.713824
TACGCTT1450.035.713823
TACGCTC750.035.713823
TACGCTA950.035.713823
ACGCTTA301.0549913E-535.713824
ACGCTGC453.6707206E-935.713824
ACGCTAG405.1968527E-835.713824
TCCGCTA251.5145446E-435.7138183
CGCTAGC251.5145446E-435.7138185
CGTAGGC1850.035.6066551
CGTAACC2050.035.6066551
CGTTAGC650.035.606651
CGTACGC4850.035.606651
CGTAAGT405.3363692E-835.606651
GTAAGTT357.5636854E-735.606652
GTACGCT4950.035.606652
CGTACGG453.77986E-935.6066471
Produced by FastQC (version 0.11.5)