FastQCFastQC Report
Thu 26 May 2016
SRR2094182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7700388
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT388850.5049745545289406No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA370650.48133938185971925No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC317740.4126285584570543No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT283830.3685918164123678No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA263360.34200874033879847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225540.2928943320778122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199780.2594414722998374No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC172090.2234822453102363No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC156500.20323651223808464No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT151890.1972498009191225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140740.18277001106957208No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTC138110.1793545987552835RNA PCR Primer, Index 31 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA137350.17836763550096438No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT125520.1630047732659705No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC114470.14865484700251466No Hit
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGC112360.14591472533591812No Hit
GCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACC109360.14201881775307945No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT108940.14147339069148204No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC90260.1172148728090065No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG88600.11505913727983579No Hit
CTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG86000.11168268404137557No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC84790.11011133464963063No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGT83040.10783872189297475No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG79290.10296883741442639No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAAC78500.10194291508427887No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG77620.1008001155266462RNA PCR Primer, Index 31 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG81000.020.16610528
CGCTATC81250.020.1608833
TATCTCG82250.019.97034636
GCTACGC82600.019.85758229
GACGCTA83100.019.84601826
ACGCTAC82950.019.77340927
TATGCCG81250.019.71662943
GAGACGC83750.019.69173224
ACGCTAT83700.019.67827632
CTCGTAT81900.019.66952739
TCTCGTA83300.019.44799638
TACGCTA84350.019.44559931
TGCCGTC82800.019.37735445
GCCCACG86650.019.36936218
AGACGCT85800.019.24746525
CGTATGC83850.019.15880441
CCCACGA87500.019.12977819
TCGTATG84600.019.09496540
ATGCCGT83950.019.08510844
CTACGCT86000.019.01981730
Produced by FastQC (version 0.11.5)