FastQCFastQC Report
Thu 26 May 2016
SRR2094181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746752
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81730.21813560118203712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71310.19032484669388314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53180.1419362690671814No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT48490.1294187605691543No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA39990.10673244452795382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1151.5992009E-719.5635285
GCGCATA1209.7687E-514.99870511
ATAACGC1605.0379476E-614.0612873
ATCTCCG12700.013.99485610
TACCTGG29800.013.8914182
CGAGACC12600.013.74936323
ACCTGGG29550.013.6282663
GGACCGT2154.084177E-813.6034776
TCTCGTA12350.013.300438
CTCGTAT12050.013.25717939
GTACCCG2907.094059E-1113.2080811
ACGAGAC13000.013.15306322
TATACTG13600.013.0687265
TATGCCG12250.013.04073443
TCTCCGA13550.012.95091211
TAGGACT8700.012.9299184
GTACCTG33300.012.9234341
TCGTATG12550.012.908113540
GTATTAT9450.012.8751051
TAGTACT6850.012.8091144
Produced by FastQC (version 0.11.5)