FastQCFastQC Report
Thu 26 May 2016
SRR2094180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5017869
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT119190.23753111131438465No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA111000.2212094416972623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103470.2062030714632048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.1782429951838121No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC83430.16626579928650986RNA PCR Primer, Index 39 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT77640.15472703651689593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70050.13960109361165068No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA68340.13619327248280097No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA56040.11168087488932055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG28300.016.53532810
CGAGACC29050.016.10938823
TATGCCG28150.016.06591643
TCTCGTA28500.016.0269938
CTCGTAT28400.015.92401339
ATGCCGT29850.015.37906544
TGCCGTC30100.015.32609245
ATCTCGT30100.015.32456637
CCCACGA31400.014.97525219
TCCGAGC31700.014.90376213
CCACGAG31200.014.85506220
ACGAGAC31050.014.85421822
CACGAGA31250.014.83114621
TCGTATG30650.014.82844940
GCCCACG31750.014.81017118
TCTCCGA31750.014.73857411
CATCTCG31300.014.66544736
CGTATGC31300.014.6645741
CTCCGAG32800.014.4725312
CGAGCCC32450.014.28240615
Produced by FastQC (version 0.11.5)