FastQCFastQC Report
Thu 26 May 2016
SRR2094179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837933
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT76380.9115287260437289No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC55380.660912029959436TruSeq Adapter, Index 1 (95% over 22bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA33720.402418809141065TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG32890.39251348258154295RNA PCR Primer, Index 1 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.39191677616229453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.3366617617399004No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT27990.3340362534952079No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT23740.2833162078591009No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC23580.2814067473175063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.2688759125132916No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21450.255987053857528No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17880.21338221552319814No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA16790.20037401558358486No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTT15040.17948929090989377TruSeq Adapter, Index 1 (96% over 27bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12130.14476097730964171No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA10450.12471164162289824RNA PCR Primer, Index 1 (96% over 28bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA9890.1180285297273171No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA8430.10060470228526625No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC8380.10000799586601793TruSeq Adapter, Index 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA6600.022.84287845
CTGCTTG7750.019.16294344
ACGTAGG951.3161893E-518.9456127
CTTATAC8450.018.6529061
TACGTAG1002.0461432E-517.99940326
ACTATAC1752.6866473E-916.7137323
TAACACC1358.573188E-716.6671094
TATATCC952.8189234E-416.5783983
TATACAC11800.016.0164183
TTATACA10300.015.7285712
TCTGCTT9600.015.46731543
GGCTTAC1502.570774E-615.0003987
CAAGACG900.003752128215.0003984
TATACTG3000.015.0003985
AAACGAT900.00375510914.99860843
CGTAGGC1209.754847E-514.99860828
GAGTACT45250.014.81630411
TTCTGCT10600.014.645740542
TACACAT18450.014.512582
CGTCTTC18050.014.461081545
Produced by FastQC (version 0.11.5)