FastQCFastQC Report
Thu 26 May 2016
SRR2094178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5473620
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129800.2371373971886978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111070.20291872654659987No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC83390.1523489025544338RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.14747096071703916No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT75800.13848239373577267No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA64570.11796580690658102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC3150.016.428133
CGTCGTA1956.493792E-1016.15410
TATCTCG24900.015.36117236
TATACCG2405.0931703E-1115.0006915
TATAACG3000.014.9997332
ATCTCCG26250.014.91442810
TCTCGTA25300.014.8519238
GACGGAC25900.014.76807526
TATGCCG25900.014.6810743
ACGGACT25850.014.6224327
CTCGTAT25450.014.58729939
CGGACTC26100.014.56817328
TACCTGG37100.014.494352
CGAGACG26700.014.49412323
ACGAGAC27400.014.12383522
TCGTATG27050.014.05692240
ATGCCGT26700.013.98813144
CACGAGA27700.013.97061421
GTACCCG3900.013.8608471
TCTCCGA28500.013.81478511
Produced by FastQC (version 0.11.5)