FastQCFastQC Report
Thu 26 May 2016
SRR2094177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6147072
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT140010.2277669758870565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127430.20730194798434118No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC123010.20011153277527902No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA120770.1964675214476095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.1750101511744128No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT89360.1453700233216725No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA83610.1360159763868066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.12752412856071965No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT64390.10474905776278527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA38700.015.63942138
TATGCCG39150.015.5742243
TGCCGTC39950.015.20602745
ATGCCGT39850.015.18772544
CTCGTAT39950.015.14995539
ATCTCCG40450.015.07442510
TCGTATG40400.015.03665240
CGAGACT40300.015.01813523
CACGAGA40700.014.87053821
ATCTCGT42650.014.45499537
CGTATGC42200.014.44859541
CGTATAC2509.640644E-1114.3996633
CATCTCG42900.014.37040736
ACGAGAC42750.014.26270722
CCACGAG43250.014.04545620
TACCTGG45050.013.9841332
CCCACGA43200.013.957211519
TCCGAGC43450.013.92868413
GCCCACG44150.013.75880118
TCTCCGA45200.013.58850911
Produced by FastQC (version 0.11.5)