FastQCFastQC Report
Thu 26 May 2016
SRR2094176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523095
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA324651.2867133421452621No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA94160.3731924481638622No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC82730.327890943464277No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT80540.31921112760320164No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA76180.3019307636058095No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA75310.29848261757880695No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG69300.27466266628882385No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58970.233720886450966No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT58460.23169955946961965No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT55690.22072097959054257No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA52920.2097423997114655No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC48460.19206569709027999TruSeq Adapter, Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC47070.1865565902195518No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA46020.18239503466972112No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT45430.18005663678934009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.17862981774368383No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT44480.17629141986330282No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT41020.1625781034800513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39480.15647448867363298No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA34330.13606304954827306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34230.13566671092447966No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA29980.1188223194132603TruSeq Adapter, Index 11 (96% over 28bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT28490.1129168739187387No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG27490.10895348768080472No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT27250.10800227498370057No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC26520.10510900303000877No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG26430.10475229826859474RNA PCR Primer, Index 11 (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA25670.10174012472776492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA952.8055845E-416.5908871
CCCGCAC1056.04103E-414.9997963
TAAGACG1108.5942436E-414.3179874
CTTACAC5900.014.1099763
TATCACG1150.001202599513.6954642
TATACAG13950.012.9030495
TGCTTGA11200.012.85747745
CGTCTTC19150.012.33713545
TACACGG2207.9322854E-712.272565
ATCTCCG22300.012.20811210
GAATGCG1300.003024427512.11473911
GCGGTCT1506.4522674E-412.00031237
CCGTGCA1506.4554927E-411.99959859
TACACTG21950.011.8904985
CCGTCTT19350.011.86077244
ATCACGC1350.004009763711.6665083
CTACACT6750.011.6665074
TAGGGTG4250.011.64695
CTAATAC5450.011.5594753
GCCGTCT19850.011.33463443
Produced by FastQC (version 0.11.5)