FastQCFastQC Report
Thu 26 May 2016
SRR2094174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866836
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97110.19953415319521758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87850.1805074179610737No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC79490.16332993345163058No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT66460.13655689240401772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.13398848861971105No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA57870.11890682159826219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATA1305.795264E-717.30678421
GACGTAG24350.014.96872126
ATCTCCG24650.014.968019510
AATCTCG24650.014.4227836
CGAGACG25800.014.38863123
ACGTAGA24950.014.3382227
CTCGTAT24800.014.33407239
AGACGTA25550.014.26539625
ATAACGC1758.1938197E-714.1413873
TATGCCG25650.014.12337243
TCTCGTA25400.014.08535338
GCCCACG27000.013.83232318
ACGAGAC26700.013.73523322
CGTATGC26550.013.644052541
CACGAGA27100.013.61552121
TACCTGG36050.013.6046892
TCGTATG26550.013.55847140
TCCGAGC26800.013.51538713
ATGCCGT26650.013.50995544
TGCCGTC26350.013.4929745
Produced by FastQC (version 0.11.5)