FastQCFastQC Report
Thu 26 May 2016
SRR2094173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878487
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92920.19046888922733626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81690.1674494571780144No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT77230.15830727846563905No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT71140.1458238999099516No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA65190.1336274955739351No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA64760.13274607475637426No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC63660.13049127731610233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60130.12325542734868414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1608.1854523E-1018.279585
ATCTCCG22700.017.04690210
CGCGTAA800.001712966416.87345910
TATGCCG22650.016.49008243
AATCTCG23000.016.4354736
CTCGTAT22950.016.17615339
TCGTATG23150.016.13342940
TCTCGTA22950.016.07910538
CACGAGA24300.015.92528821
CGAGACA24200.015.8049923
CGAGCCC24700.015.66658515
ATGCCGT23500.015.60831644
CCACGAG25000.015.56905720
CCCACGA24900.015.54090919
GCCCACG24900.015.45055518
TGCCGTC23600.015.4468345
ACGAGAC24900.015.3608322
TCCGAGC25050.015.35787813
CCGAGCC26000.015.31590814
TCTCCGA25500.015.26331111
Produced by FastQC (version 0.11.5)