FastQCFastQC Report
Thu 26 May 2016
SRR2094172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5998860
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC132120.22024184595073065No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT131840.21977509060054742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129100.21520755610232611No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115150.19195313776284162No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA114920.19156973158233398No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT103580.1726661398999143No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA93990.15667976915613968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78640.13109157406573915No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA67600.11268807740137292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC32200.019.4966633
CGCTACG32700.019.19662528
ACGCTAC33450.018.76620727
GACGCTA33450.018.76620726
TATCTCG33350.018.62210536
TCTCGTA33100.018.49067738
CTCGTAT33300.018.31021939
GCTACGC34300.018.30115729
CGAGACG35150.018.24204323
TATGCCG33450.018.0947943
ACGCTAT34850.018.078732
TACGCTA34650.018.05181531
TCGTATG33850.018.01286140
ATGCCGT34400.017.72796644
GAGACGC35350.017.69346624
CTACGCT35450.017.64370330
CGTATGC35150.017.41169241
CCCACGA37050.017.36670119
ACGAGAC36800.017.3629922
TGCCGTC34850.017.30533845
Produced by FastQC (version 0.11.5)