FastQCFastQC Report
Thu 26 May 2016
SRR2094169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4701631
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121000.2573574999824529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105720.22485813965409024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT83480.1775554057730179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72450.154095461766353No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA68160.14497096858515693No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC63430.13491062994947922No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT54630.11619372085984629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT18050.018.69751439
TATGCCG18050.018.57286343
TCTCGTA18600.018.02366638
AATCTCG19400.017.97627836
CACGAGA19450.017.5830321
TCGTATG19450.017.58265940
ACGAGAC19750.017.54416322
ATGCCGT19600.017.3334944
ATCTCCG19750.017.31594810
TGCCGTC19800.017.27203645
CCCACGA20000.017.2110819
CCACGAG20050.017.16907320
CGAGACA20750.016.80709323
CGTATGC20500.016.68208341
GCCCACG20700.016.62923818
CGAGCCC21150.016.48817815
TCCGAGC20950.016.43080113
ATCTCGT20450.016.39351337
CCGAGCC21600.016.24883314
TCTCCGA21500.016.01030711
Produced by FastQC (version 0.11.5)