FastQCFastQC Report
Thu 26 May 2016
SRR2094168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4689905
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT96400.20554787357099985No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT91590.19529180228597381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.1927331150630983No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA87030.18556879083904684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC77670.16561103050061782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.16219944753678378No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA75710.16143184136992114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.1239683959483188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA600.006513391618.75001338
TATACCG1454.782123E-918.6211015
ATCTCCG27900.015.48355210
GACCGTA26600.015.4788426
CGTACTA27100.015.27643929
ACCGTAC27100.015.27627627
TCTCGTA27900.015.08065738
CTCGTAT28100.014.97268139
TACCTGG33300.014.66185952
TATGCCG28850.014.50530243
CACGAGA29200.014.40864321
ACGAGAC29250.014.38416622
CGAGCCC30200.014.3782315
CGAGACC29050.014.32829523
ATGCCGT29250.014.23017244
CCACGAG29700.014.16577120
CCCACGA29750.014.14196419
CCGTACT29450.014.05698528
TCTCCGA30750.014.04789111
TGCCGTC29400.014.00451645
Produced by FastQC (version 0.11.5)