FastQCFastQC Report
Thu 26 May 2016
SRR2094167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6307812
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA634641.006117493672925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA328840.521321814917756No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT229300.3635174922778294No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT212730.3372484785532606No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC162660.2578707165020137No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT146990.2330285049712959No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA144730.22944564612895885No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT118980.18862325002710925No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAG118530.18790984893018373No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA115840.18364529570634No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT105430.16714195033079615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101430.1608006072470137No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT97410.15442755744781234No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT87830.13924004076215332No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC87000.1379242120722685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83920.133041377897756No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCA77840.12340253641040665No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA74480.11807580822002939No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT68630.10880159395999754No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC65710.10417241350883634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG1751.4006218E-1017.99959441
TAGCGCG1406.488881E-817.6787344
CGATCGT1652.3645043E-816.36326842
GCGAATC27500.015.79055333
GGCGAAT27450.015.65538432
AATCTCG28500.015.23661936
CGAATCT29150.015.05111934
CGCGCGT1502.577699E-615.0001377
TATGCCG28700.014.89501643
ATGCCGT29150.014.8192144
TCTCGTA28400.014.81527638
TAAGGCG29250.014.69139429
ATCTCCG30550.014.655622510
CTCGTAT29600.014.5941539
CACGAGA30850.014.513679521
TCCGAGC30450.014.48162813
CGAGACT30050.014.45093323
AAGGCGA30000.014.39910430
TGCCGTC30250.014.35470745
ACGAGAC31400.014.18791622
Produced by FastQC (version 0.11.5)