FastQCFastQC Report
Thu 26 May 2016
SRR2094165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643199
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT133380.2363553013104801No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC132190.23424656830283674No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA120110.2128402702084403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119190.21120998922774123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100820.178657530950087No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT88980.15767652354630768No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA84590.14989724799710236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73520.13028071489238638No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC60970.10804155586219802TruSeq Adapter, Index 10 (95% over 22bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT58200.10313299247465843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG41300.015.96066810
CTCGTAT42900.015.1577839
TATGCCG43050.015.05203343
ACGAGAC43500.014.99976822
TCTCGTA43950.014.84776838
TCGTATG43950.014.84658340
CGAGACC44450.014.72954723
CACGAGA45050.014.58357121
TCTCCGA45500.014.48737611
CGTATGC45200.014.43625941
CCCACGA45450.014.40469819
GACCGAG45850.014.37805926
GAGACCG46400.014.35297324
ACCTGGG39500.014.352813
ATGCCGT44550.014.29486344
ATAACGC3150.014.2841033
CCACGAG45900.014.26398120
GATCTCG45700.014.229959536
TCCGAGC46450.014.19107913
CGAGGCT45900.014.16619829
Produced by FastQC (version 0.11.5)