FastQCFastQC Report
Thu 26 May 2016
SRR2094164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6825814
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT176640.25878232251860367No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA165590.24259377709383817No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT148440.2174685685839081No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA137980.2021443889329536No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC107150.15697761468449037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.1527876382216099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93550.13705325108477906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG27600.019.40181728
CGCTATC27800.019.26350633
TATCTCG28600.018.88298436
TCTCGTA29100.018.32628638
GACGCTA29100.018.3245426
ACGCTAC29450.018.25942627
ACGCTAT29750.018.00085632
GCTACGC29850.017.939529
TACGCTA29800.017.89409631
TATGCCG29350.017.86181343
CGAGACG30400.017.762723
CTCGTAT29900.017.7591339
GAGACGC30750.017.56065424
CCACGAG31800.017.12157420
CACGAGA31550.017.04356221
TGCCGTC30550.016.94161445
ACGAGAC31900.016.92709222
CGTACGA800.001712225316.8746928
GCCCACG32700.016.78732918
CCCACGA32300.016.78626419
Produced by FastQC (version 0.11.5)