FastQCFastQC Report
Thu 26 May 2016
SRR2094163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5982980
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT209130.3495415328147512No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT197020.329300783221739No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA195190.3262421067762219No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA175550.29341565574345896No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC106100.17733637752424378RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92560.15470551464320456No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT86040.14380793517611623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83200.1390611367579367No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC81520.13625317149647834No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGT79900.13354549070864352No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTT65580.10961092967049864No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC60630.10133746059655889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG31300.017.8258810
CTCGTAT30150.017.68616739
TCTCGTA30450.017.58639538
TATGCCG31000.017.20136643
CCCACGA32900.016.95896719
ATGCCGT32150.016.86840844
TCGTATG32000.016.80430640
GATCTCG32100.016.68256236
CACGAGA32600.016.63208621
GCCCACG33850.016.54947918
CCACGAG33400.016.43579320
ATCTCGT32050.016.42763337
ACGAGAC33600.016.40491922
TGCCGTC32900.016.34707545
CGAGACC33100.016.31328423
CGTATGC33550.016.22954841
TCTCCGA34350.016.11209511
TCCGAGC34500.016.10725413
CGAGCCC34800.016.03304915
CCGAGCC36700.015.63211614
Produced by FastQC (version 0.11.5)