FastQCFastQC Report
Thu 26 May 2016
SRR2094162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6932784
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT194480.28052222599175164No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA174010.2509958481325828No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC168780.24345198119543318RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137260.19798684049582388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA128360.18514928490488092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116250.16768155476933944No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTAT97700.14092462710507062No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA89570.1291977364360407No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT85380.12315398835446192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80740.11646115038345345No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC69330.10000311563146927RNA PCR Primer, Index 39 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG44700.017.61522710
TCTCGTA44300.017.5232738
CTCGTAT44750.017.34580839
TATGCCG45200.017.074343
CGAGACC46150.016.91674423
ACGAGAC47150.016.79654722
ATGCCGT46550.016.7252244
CACGAGA47050.016.6887921
TGCCGTC47150.016.60783245
ATCTCGT47300.016.41185837
TCGTATG48150.016.21419340
CATCTCG48850.016.07547436
TCCGAGC48750.016.01336513
GCCCACG49200.016.00465618
CCACGAG48950.015.94897120
CGAGCCC49400.015.93951515
CCCACGA49200.015.913219
CCGAGCC51150.015.61409114
TCTCCGA50800.015.54429911
CTCCGAG51100.015.40901612
Produced by FastQC (version 0.11.5)