FastQCFastQC Report
Thu 26 May 2016
SRR2094160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2094160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6186331
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT119980.1939437123555141No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113040.18272543127744054No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC110800.17910454516578567No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA103380.1671103599209289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96240.155568785440029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64940.10497336789770867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG31800.018.32478710
TATGCCG32150.017.70540443
CTCGTAT32350.017.66534839
CGAGACT33650.017.45204423
CATCTCG33300.017.431536
TCTCGTA32700.017.40788838
ATGCCGT33200.017.21279544
ACGAGAC34650.016.9483822
TGCCGTC34000.016.94013245
TCGTATG33900.016.92387440
CACGAGA34550.016.80151621
TCTCCGA35600.016.7470211
CCGAGCC36000.016.5614814
TCCGAGC35600.016.4947713
CCCACGA35500.016.41421719
CCACGAG35800.016.27732520
CGTATGC35550.016.20179641
ATCTCGT36150.015.93340137
CGAGCCC36750.015.85616715
CTCCGAG37750.015.61467912
Produced by FastQC (version 0.11.5)